Lectins

Brown, Jay C.; Hunt, Richard C.

doi:10.1016/s0074-7696(08)60758-5

Cited by 198 publications

(60 citation statements)

References 446 publications

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“…The use of lectins that specifically bind to sugars is another principle for analyzing cell surfaces, and gives information on the carbohydrate pattern of the cell membrane (2,5,6). Lectins can be labeled with fluorescein-isothiocyanate (FITC).…”

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confidence: 99%

Flow cytometric analysis of the binding of eleven lectins to human T‐ and B‐cells and to human T‐ and B‐cell lines

et al. 1984

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The relative surface binding of 11 lectins to human peripheral blood T‐ and B‐lymphocytes, to Molt‐4 and JM T‐cell lines, and to 6410 and NC37 B‐cell lines was determined by flow cytometry. The lectins from Lens culinaris (LCA), Ricinus communis (RCA), Arachis hypogaea (PNA), Abrus precatorius (APA), Ulex europaeus (UEA‐F), Sarothamnus scoparius (SAS‐F), Helix pomatia (HPA), Phaseolus coccineus (L‐PHA), Glycine max (SBA), and Triticum vulgare (WGA) were fluoresceinated and incubated with living, formaldehyde‐fixed, or neuraminidase‐treated cells. Except LCA, which preferentially bound to the two B‐cell lines tested in this study, none of the other lectins exhibited selective binding to the undifferentiated cells of the cell lines. The T‐cell lines and, in part, the peripheral blood T‐cells bound less WGA, APA, LCA, and L‐PHA than the B‐cell lines and the peripheral blood B‐cells. Binding of PNA was found only after neuraminidase treatment of the cells; the binding of PNA, HPA, and UEA‐F after neuraminidase treatment was higher for the T‐cells than the B‐cells from peripheral blood. No significant differences were detected between both cell types for RCA, ConA, SBA, and SAS‐F.

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confidence: 99%

Flow cytometric analysis of the binding of eleven lectins to human T‐ and B‐cells and to human T‐ and B‐cell lines

et al. 1984

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“…Lectins are the saccharide-binding proteins or glycoproteins isolated from plants or animals, and bind to mono-or oligosaccharides with great specificity through complementary sugar-binding sites (2,6) . Several histochemical studies of the kidney using lectins have also been reported.…”

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confidence: 99%

Distribution of glycoconjugates in normal rat kidney determined by using horseradish peroxidase-labeled lectins.

Hamada

1983

Acta Histochem. Cytochem

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“…Generally, lectins are classified in five groups on the basis of their affinity for (i) glucose/mannose; (ii) galactose and N-acetyl-D-galactosamine; (iii) N-acetylglucosamine; (iv) L-fucose, and (v) sialic acids (31). Binding is reversible and noncovalent with simple or complex carbohydrate conjugates, whether free in solution or on cell surfaces (20,107). Glycoconjugate-containing surfaces are called lectin receptors.…”

Section: Overview Of Lectinsmentioning

confidence: 99%

“…These include ammonium sulfate or ethanol precipitation and the use of affinity chromatography (20,107).…”

Section: Overview Of Lectinsmentioning

confidence: 99%

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Lectins and their application to clinical microbiology

1990

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Lectins are generally associated with plant or animal components, selectively bind carbohydrates, and interact with procaryotic and eucaryotic cells. Lectins have various specificities that are associated with their ability to interact with acetylaminocarbohydrates, aminocarbohydrates, sialic acids, hexoses, pentoses, and as other carbohydrates. Microbial surfaces generally contain many of the sugar residues that react with lectins. Lectins are presently used in the clinical laboratory to type blood cells and are used in a wide spectrum of applications, including, in part, as carriers of chemotherapeutic agents, as mitogens, for fractionation of animal cells, and for investigations of cellular surfaces. Numerous studies have shown that lectins can be used to identify rapidly certain microorganisms isolated from a clinical specimen or directly in a clinical specimen. Lectins have been demonstrated to be important diagnostic reagents in the major realms of clinical microbiology. Thus, they have been applied in bacteriology, mycology, mycobacteriology, and virology for the identification and/or differentiation of various microorganisms. Lectins have been used successfully as epidemiologic as well as taxonomic markers of specific microorganisms. Lectins provide the clinical microbiologist with cost-effective and potential diagnostic reagents. This review describes the applications of lectins in clinical microbiology.

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Lectins

Cited by 198 publications

References 446 publications

Flow cytometric analysis of the binding of eleven lectins to human T‐ and B‐cells and to human T‐ and B‐cell lines

Flow cytometric analysis of the binding of eleven lectins to human T‐ and B‐cells and to human T‐ and B‐cell lines

Distribution of glycoconjugates in normal rat kidney determined by using horseradish peroxidase-labeled lectins.

Lectins and their application to clinical microbiology

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