Lectins as Biomarkers of IC/BPS Disease: A Comparative Study of Glycosylation Patterns in Human Pathologic Urothelium and IC/BPS Experimental Models

Peskar, Dominika; Kuret, Tadeja; Jeruc, Jera; Erman, Andreja

doi:10.3390/diagnostics12051078

Cited by 4 publications

(6 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Chronic inflammatory conditions can significantly affect the glycosylation profile of urothelial cells, contributing to impaired barrier function [3,35]. The importance of restoring a deficient urothelial glycocalyx is also reflected in the use of glycosaminoglycan replenishment as one of the most common and successful therapy options used for IC/BPS [48].…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Sex-Dependent Differences in Blood–Urine Barrier Are Subtle but Significant in Healthy and Chronically Inflamed Mouse Bladders

Peskar,

Kerec Kos,

Cerkvenik

et al. 2023

IJMS

Self Cite

View full text Add to dashboard Cite

The urothelium is a vital permeability barrier that prevents the uncontrolled flow of urinary components into and out of the bladder interstitium. Our study addressed the question of possible sex-specific variations in the urothelium of healthy mice and their impact on chronic bladder inflammation. We found that healthy female bladders have a less robust barrier function than male bladders, as indicated by significant differences in transepithelial electrical resistance (TEER) values. These differences could be attributed to detected higher claudin 2 mRNA expression and a less pronounced glycocalyx in females than in males. In addition, TEER measurements showed delayed barrier recovery in chronically inflamed female bladders. We found subtle differences in the expressions of genes involved in the regulation of the actin cytoskeleton between the sexes, as well as pronounced urothelial hyperplasia in females compensating for attenuated barrier function. The identified genetic variations in glycosylation pathways may also contribute to this divergence. Our findings add to the growing body of literature on the intricate sex-specific nuances of urothelial permeability function and their implications for chronic bladder inflammation. Understanding these differences could lead to tailored diagnostic and therapeutic approaches in the treatment of bladder disorders in the future.

show abstract

Section: Discussionmentioning

confidence: 99%

“…The glycosylation profile of urothelial cells is altered in a chronically inflamed bladder, and a loss of the protective GAG layer may contribute significantly to an increased permeability of the urothelium [3,35].…”

Section: Urothelial Glycosylation Pattern Differs Between the Sexesmentioning

confidence: 99%

Sex-Dependent Differences in Blood–Urine Barrier Are Subtle but Significant in Healthy and Chronically Inflamed Mouse Bladders

Peskar,

Kerec Kos,

Cerkvenik

et al. 2023

IJMS

Self Cite

View full text Add to dashboard Cite

show abstract

“…Furthermore, IC/BPS cell models could also originate from cancer cells. For example, human carcinoma epithelial RT4 cells and T24 cells were treated with TNF-α to mimic the inflammatory condition in IC/BPS [ 160 , 161 ]. Ketamine has been successfully used as a stimulus to induce dysregulation in SV-HCU-1, RT4, and 5637 cell lines [ 162 ].…”

Section: The Fundamental Research Of Ic/bpsmentioning

confidence: 99%

Broaden Horizons: The Advancement of Interstitial Cystitis/Bladder Pain Syndrome

2022

IJMS

View full text Add to dashboard Cite

Interstitial cystitis/bladder pain syndrome (IC/BPS) is a debilitating disease that induces mental stress, lower urinary symptoms, and pelvic pain, therefore resulting in a decline in quality of life. The present diagnoses and treatments still lead to unsatisfactory outcomes, and novel diagnostic and therapeutic modalities are needed. Although our understanding of the etiology and pathophysiology of IC/BPS is growing, the altered permeability of the impaired urothelium, the sensitized nerves on the bladder wall, and the chronic or intermittent sensory pain with inaccurate location, as well as pathologic angiogenesis, fibrosis, and Hunner lesions, all act as barriers to better diagnoses and treatments. This study aimed to summarize the comprehensive information on IC/BPS research, thereby promoting the progress of IC/BPS in the aspects of diagnosis, treatment, and prognosis. According to diverse international guidelines, the etiology of IC/BPS is associated with multiple factors, while the presence of Hunner lesions could largely distinguish the pathology, diagnosis, and treatment of non-Hunner lesions in IC/BPS patients. On the basis of the diagnosis of exclusion, the diverse present diagnostic and therapeutic procedures are undergoing a transition from a single approach to multimodal strategies targeting different potential phenotypes recommended by different guidelines. Investigations into the mechanisms involved in urinary symptoms, pain sensation, and bladder fibrosis indicate the pathophysiology of IC/BPS for further potential strategies, both in diagnosis and treatment. An overview of IC/BPS in terms of epidemiology, etiology, pathology, diagnosis, treatment, and fundamental research is provided with the latest evidence. On the basis of shared decision-making, a multimodal strategy of diagnosis and treatment targeting potential phenotypes for individual patients with IC/BPS would be of great benefit for the entire process of management. The complexity and emerging evidence on IC/BPS elicit more relevant studies and research and could optimize the management of IC/BPS patients.

show abstract

“…Particularly, potassium ions are suspected to provoke nerve terminals of C-fibres triggering pain and OAB symptoms [12]. Using an animal model with cyclophosphamide (CYP) induced cystitis, Peskar et al [13 ▪ ] observed that changes in glycosylation patterns impairing the GAG-layer function may contribute to BPS/IC disease. The positive effect of restoring the GAG layer in patients with HIC or glomerulations using orally administrated pentosane polysulfate sodium could be demonstrated in a recently published systematic review and meta-analysis [14 ▪▪ ].…”

Section: Introductionmentioning

confidence: 99%

Latest insights into the pathophysiology of bladder pain syndrome/interstitial cystitis

Tornic,

Engeler

2023

Current Opinion in Urology

View full text Add to dashboard Cite

Purpose of review Bladder pain syndrome/interstitial cystitis (BPS/IC) is a common medical problem in both sexes affecting people of all ages. Patients might report overactive bladder symptoms with additional bladder pain at maximum bladder filling, during and after micturition. This review aims to highlight pathophysiological mechanisms associated with this disease. Recent findings Latest literature exposes different pathophysiological mechanisms such as impaired urothelial barrier function, alteration of urothelial factors and cytokines, chronic inflammation, vascular lesions, neurogenic inflammation and processes in the central nervous system leading to central sensitization. According to the involved mechanisms, BPS/IC may be arranged in clusters according to the clinical phenotype thus helping in clinical decision-making and treatment. Moreover, patients with BPS/IC suffer from other comorbidities such as fibromyalgia, irritable bowel syndrome, chronic pain and functional syndromes and psychosomatic diseases making the management challenging for medical professionals. Summary Bladder pain syndrome/interstitial cystitis is a complex heterogeneous medical condition involving different pathomechanisms leading to bladder pain and dysfunction, consequently, impairing quality-of-life in affected individuals. However, these mechanisms are still not fully understood, so that patient treatments often remain unsatisfactory. For this reason, continuing research is important to understand the underlying pathomechanisms to discover biomarkers and treatment targets eventually improving diagnostic and therapeutic measures of BPS/IC.

show abstract

Lectins as Biomarkers of IC/BPS Disease: A Comparative Study of Glycosylation Patterns in Human Pathologic Urothelium and IC/BPS Experimental Models

Cited by 4 publications

References 50 publications

Sex-Dependent Differences in Blood–Urine Barrier Are Subtle but Significant in Healthy and Chronically Inflamed Mouse Bladders

Sex-Dependent Differences in Blood–Urine Barrier Are Subtle but Significant in Healthy and Chronically Inflamed Mouse Bladders

Broaden Horizons: The Advancement of Interstitial Cystitis/Bladder Pain Syndrome

Latest insights into the pathophysiology of bladder pain syndrome/interstitial cystitis

Contact Info

Product

Resources

About