Lectins and/or xyloglucans/alginate layers as supports for immobilization of dengue virus particles

Pereira, Edla M. A.; Sierakowski, Maria Rita; Jó, Tatiane A.; Moreira, Renato A.; Monteiro-Moreira, Ana Cristina de Oliveira; Franca, Rafael Freitas Oliveira; Fonseca, Benedito Antônio Lopes da; Petri, Denise Freitas Siqueira

doi:10.1016/j.colsurfb.2008.05.013

Cited by 16 publications

(12 citation statements)

References 36 publications

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“…2) shows that GML is totally covered by lectin, which appears as densely packed small globules. Similar morphological features have been observed for ConA adsorbed onto polysaccharide surfaces, such as carboxymethyl cellulose (CMC) [18], cashew gum [2], xyloglucan-amino terminated films [1] and bare Si wafers [19] where the average thickness was Desorption experiments were performed by dipping GMLConA-covered wafers into pure water for 24 h. After desorption experiments, ellipsometric measurements were again performed on three different areas of the same sample. Negligible changes in the average thickness values were observed indicating that ConA adsorbed irreversibly onto GML-amino terminated films.…”

Section: Adsorption Behaviour Of Cona Onto Gml Thin Filmsmentioning

confidence: 75%

“…The thickness of the polysaccharide, ConA or dengue virus layer was determined in air, assuming the indices of refraction as 1.50, 1.52 [18] and 1.50 [1,19], respectively. Ellipsometric analyses, in triplicate, were performed in three different areas of the sample to test for film heterogeneity and were ultimately averaged.…”

Section: Ellipsometric Measurementsmentioning

confidence: 99%

“…Favourable interactions between ConA and sugar-containing polymers, also, contribute for multilayer film construction [17]. Recent studies showed the irreversible adsorption of ConA monolayer onto a blend of xyloglucan and alginate [1]. In another study, the adsorption onto cashew gum amino-terminated films [2] demonstrated that although gum is predominantly composed of galactose, the presence of glucose as a side-chain favoured the interaction with ConA.…”

Section: Introductionmentioning

confidence: 97%

“…Fabrication of multilayer films utilizing carbohydrate polymers has been reported for xyloglucan [1], cashew gum [2], chitosan [3], carrageenan [4], alginate [5], amylose [6], and oxidized galactomannan [7].…”

Section: Introductionmentioning

confidence: 99%

“…Recent studies revealed that ConA is specifically recognized by the envelope protein (Eprot) located at the surface of dengue virus [1,19,20]. Dengue virus is an arthropod-born virus and a member of the family Flaviviridae that cause infections with a wide spectrum of clinical illness, which is important for public health.…”

Section: Introductionmentioning

confidence: 99%

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Galactomannan thin films as supports for the immobilization of Concanavalin A and/or dengue viruses

Valenga

Petri

Lucyszyn

et al. 2012

International Journal of Biological Macromolecules

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The immobilization of the glucose/mannose-binding lectin from Concanavalia ensiformis seeds (ConA) onto a monolayer made of a galactomannan extracted from Leucaena leucocephala seeds (GML), which was adsorbed onto - amino-terminated surfaces, was investigated by means of ellipsometry and atomic force microscopy. The mean thickness of GML monolayer, which polysaccharide consists of linear 1→4-linked β-D-mannopyranosil units partially substituted at C-6 by α-D-galactopyranosyl units, amounted to (1.5±0.2) nm. ConA molecules adsorbed onto GML surfaces forming (2.0±0.5) nm thick layers. However, in the presence of mannose the adsorption failed, indicating that ConA binding sites were blocked by mannose and were no longer available for mannose units present in the GML backbone. The GML film was also used as support for the adsorption of three serotypes of dengue virus particles (DENV-1, DENV-2 and DENV-3), where DENV-2 formed the thickest film (4±2) nm. The adsorbed layer of DENV-2 onto ConA-covered GML surfaces presented mean thickness values similar to that determined for DENV-2 onto bare GML surfaces. The addition of free mannose units prevented DENV-2 adsorption onto ConA-covered GML films by ~50%, suggesting competition between virus and mannose for ConA binding sites. This finding suggests that if ConA is also adsorbed to GML surface and its binding site is blocked by free mannose, virus particles are able to recognized GML mannose unities substituted by galactose. Interactions between polysaccharides thin films, proteins, and viruses are of great relevance since they can provide basis for the development of biotechnological devices. These results indicate that GML is a potential polysaccharide for biomaterials development, as those could involve interactions between ConA in immune system and viruses.

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