Lectin Typing of Helicobacter pylori

Ascencio, Felipe; Guruge, Janaki L.; Ljungh, Åsa; Mégraud, F; Shen, Wei; Wadström, Torkel

doi:10.1007/978-3-642-75315-2_15

Cited by 7 publications

(4 citation statements)

References 5 publications

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“…The following freeze-dried native lectins were purchased from Sigma and ICN Biomedicals (Cleveland, Ohio): Anguilla anguilla (AAA), Lotus tetragonolobus (Lotus A), and Ulex europaeus I (UEA I), specific for ␣-L-fucose; Solanum tuberosum (STA) and Triticum vulgaris (WGA), specific for ␤-N-acetylglucosamine; Glycine max (SBA), specific for ␤-N-acetylgalactosamine; Erythrina cristagali (ECA), specific for ␤-galactose and ␤-N-acetylgalactosamine; and Lens culinaris (LCA), specific for ␣-mannose and ␣-glucose. Lectins were dissolved in PBS containing 0.02% CaCl 2 and 0.02% MgCl 2 at a concentration of 0.5 mg per ml (1).…”

Section: Methodsmentioning

confidence: 99%

“…The widespread occurrence of the gastroduodenal pathogen Helicobacter pylori in the human population and the broad spectrum of clinical outcomes from this infection (5) have focused interest on differentiation of strains based on DNA typing methods (3,7,8,15,19) but also on phenotypic typing methods (1,9,16). Typing of bacterial strains based on differences in the structures of their lipopolysaccharides (LPSs) has proven valuable in typing members of the family Enterobacteriaceae.…”

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confidence: 99%

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Differentiation of Helicobacter pylori Isolates Based on Lectin Binding of Cell Extracts in an Agglutination Assay

et al. 1999

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Plant and animal lectins with various carbohydrate specificities were used to type 35 Irish clinical isolates of Helicobacter pylori and the type strain NCTC 11637 in a microtiter plate assay. Initially, a panel of eight lectins with the indicated primary specificities were used: Anguilla anguilla (AAA),Lotus tetragonolobus (Lotus A), and Ulex europaeus I (UEA I), specific for α-l-fucose;Solanum tuberosum (STA) and Triticum vulgaris(WGA), specific for β-N-acetylglucosamine; Glycine max (SBA), specific for β-N-acetylgalactosamine;Erythrina cristagali (ECA), specific for β-galactose and β-N-acetylgalactosamine; and Lens culinaris(LCA), specific for α-mannose and α-glucose. Three of the lectins (SBA, STA, and LCA) were not useful in aiding in strain discrimination. An optimized panel of five lectins (AAA, ECA, Lotus A, UEA I, and WGA) grouped all 36 strains tested into eight lectin reaction patterns. For optimal typing, pretreatment by washing bacteria with a low-pH buffer to allow protein release, followed by proteolytic degradation to eliminate autoagglutination, was used. Lectin types of treated samples were stable and reproducible. No strain proved to be untypeable by this system. Electrophoretic and immunoblotting analyses of lipopolysaccharides (LPSs) indicated that the lectins interact primarily, but not solely, with the O side chain of H. pylori LPS.

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Section: Methodsmentioning

confidence: 99%

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confidence: 99%

Differentiation of Helicobacter pylori Isolates Based on Lectin Binding of Cell Extracts in an Agglutination Assay

et al. 1999

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“…Some researchers found lectin-binding affinity of carbohydrates present on microorganisms such as Helicobacter [81,82] and Streptococcus. [83] In 2000, Khinet al…”

Section: Targeted Nanoparticlesmentioning

confidence: 99%

Mucoadhesive and muco-penetrating delivery systems for eradication of helicobacter pylori

Arora¹,

Bisen²,

Rd³

2012

Asian J Pharm

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H elicobacter pylori (H. pylori), the major culprit for peptic ulcer, has a unique way of survival in harsh acidic environment of the stomach by colonizing deep in the gastric mucosal layer. Failure of conventional therapies against H. pylori for complete eradication has major limitations like low residence time of delivery system in stomach, poor penetration of drug in gastric mucosa, acidic degradation of antibiotics, and development of antibiotics resistance. The poor penetration of antibiotics through thick viscoelastic mucosal gel results in incomplete eradication of H. pylori. Various investigators have formulated novel gastro-retentive drug delivery systems such as floating systems, mucoadhesive systems, pH-sensitive gel systems, and muco-penetrating delivery systems for increasing the concentration of antibiotic in close proximity to the site of H. pylori infection. This review summarizes the novel drug delivery approaches investigated during the last few years and suggests that a high eradication rate can be achieved by therapy comprising of muco-penetrating delivery systems of antibiotics against H. pylori.

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“…Insuffi-(ient variability in plasmid DNA content [9. 10]. surface antigens [11] and lectins [12], precludes any of these characters from serving as the bases for typing schemes. The lack of a reliable, easily applicable method for differentiation of strains has hindered the understanding of the ecology and epidemiology of this species.…”

Section: Introductionmentioning

confidence: 99%

Differentiation of strains ofHelicobacter pyloriby numerical analysis of 1-D SDS-PAGE protein patterns: Evidence for post-treatment recrudescence

et al. 1991

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SUMMARYTwenty-three pre-and post-treatment isolates of Helicobacter pylori from the antral mucosa of eight patients with dyspepsia and gastritis were compared using 1-D SDS PAGE of proteins. The protein patterns were highly reproducible and were used as the basis for two numerical analyses. The first, based on the total protein patterns, showed that a number of the strains did not cluster with their respective patient set. This was thought to be due to differences in both mobility and intensity of proteins in the major band region. The second analysis. based on partial patterns, excluding the major band region (51-68 kDa), divided the clinical isolates into clearly defined groups corresponding to the patient sets. Although there was a degree of heterogeneity with respect to protein pattern between the pre-and post-treatment isolates of some patients, there was nonetheless clear evidence that each patient was harbouring strains of only a single type. These, results suggested that patients were not being reinfected with a different strain but that there was recrudescence of the pre-treatment strain. Protein 'fingerprints ' provided a precise and reproducible means of strain differentiation, and revealed that in each patient the same strain persisted after drug therapv even though there was marked patient-to-patient strain variation.

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Lectin Typing of Helicobacter pylori

Cited by 7 publications

References 5 publications

Differentiation of Helicobacter pylori Isolates Based on Lectin Binding of Cell Extracts in an Agglutination Assay

Differentiation of Helicobacter pylori Isolates Based on Lectin Binding of Cell Extracts in an Agglutination Assay

Mucoadhesive and muco-penetrating delivery systems for eradication of helicobacter pylori

Differentiation of strains ofHelicobacter pyloriby numerical analysis of 1-D SDS-PAGE protein patterns: Evidence for post-treatment recrudescence

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