Lectin sensitized anisotropic silver nanoparticles for detection of some bacteria

Gasparyan, Vardan K.; Bazukyan, Inga

doi:10.1016/j.aca.2012.12.015

Cited by 33 publications

(11 citation statements)

References 35 publications

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“…Analyte binding to the transducer surface changes its refractive index, which in turn alters the angle of light exiting the prism (the SPR angle). Various SPR-based biosensors have been developed for the detection of whole bacterial cells using a variety of bioreceptors, including antibodies (39,40), bacteriophages (31,41), and lectins (29,42).…”

Section: Optical Biosensorsmentioning

confidence: 99%

“…Localized surface plasmon resonance (LSPR), a process where noble metal nanoparticles are used to enhance the sensitivity of the system, has been used recently (44). Recent strategies to improve the sensitivity of SPR-based bacterial sensors include transducer surface modifications (45), using nanorods for multiple detection (46), sandwichtype assays including nanoparticles for analyte capture to boost the signal (42), and the use of modified SPR systems, such as long-range SPR, which are better suited to large analytes (39). For the detection of whole bacteria, LSPR is reported to be less sensitive (47) and sometimes limited by unclear sample when a biological matrix is used (48).…”

Section: Optical Biosensorsmentioning

confidence: 99%

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Biosensors for Whole-Cell Bacterial Detection

et al. 2014

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SUMMARY Bacterial pathogens are important targets for detection and identification in medicine, food safety, public health, and security. Bacterial infection is a common cause of morbidity and mortality worldwide. In spite of the availability of antibiotics, these infections are often misdiagnosed or there is an unacceptable delay in diagnosis. Current methods of bacterial detection rely upon laboratory-based techniques such as cell culture, microscopic analysis, and biochemical assays. These procedures are time-consuming and costly and require specialist equipment and trained users. Portable stand-alone biosensors can facilitate rapid detection and diagnosis at the point of care. Biosensors will be particularly useful where a clear diagnosis informs treatment, in critical illness (e.g., meningitis) or to prevent further disease spread (e.g., in case of food-borne pathogens or sexually transmitted diseases). Detection of bacteria is also becoming increasingly important in antibioterrorism measures (e.g., anthrax detection). In this review, we discuss recent progress in the use of biosensors for the detection of whole bacterial cells for sensitive and earlier identification of bacteria without the need for sample processing. There is a particular focus on electrochemical biosensors, especially impedance-based systems, as these present key advantages in terms of ease of miniaturization, lack of reagents, sensitivity, and low cost.

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Section: Optical Biosensorsmentioning

confidence: 99%

Section: Optical Biosensorsmentioning

confidence: 99%

Biosensors for Whole-Cell Bacterial Detection

et al. 2014

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“…Lectin-sensitized anisotropic AgNPs were prepared to detect various bacterial strains, both Gram-negative and Gram-positive [79]. Major changes in the optical properties of the nanoparticles were induced following their interactions with bacteria.…”

Section: Introductionmentioning

confidence: 99%

“…c Fluorescence spectrometry of bacterial cells stained with various concent rations of Dapt-TCO and labeled with magnet of luorescent nanoparticles (data expressed as mean and standard deviation). d Bacteria tracking by magnetic resonance (data expressed as mean and standard error)(Reprinted with permission from [79]. Copyright 2011 American Chemical Society) …”

Section: Introductionmentioning

confidence: 99%

Development of nanoparticle-based optical sensors for pathogenic bacterial detection

et al. 2017

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BackgroundPathogenic bacteria contribute to various globally important diseases, killing millions of people each year. Various fields of medicine currently benefit from or may potentially benefit from the use of nanotechnology applications, in which there is growing interest. Disease-related biomarkers can be rapidly and directly detected by nanostructures, such as nanowires, nanotubes, nanoparticles, cantilevers, microarrays, and nanoarrays, as part of an accurate process characterized by lower sample consumption and considerably higher sensitivity. There is a need for accurate techniques for pathogenic bacteria identification and detection to allow the prevention and management of pathogenic diseases and to assure food safety.ConclusionThe focus of this review is on the current nanoparticle-based techniques for pathogenic bacterial identification and detection using these applications.

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“…The main characteristics of these methods are their simplicity, low cost, and high sampling rate without the use of expensive equipment. The methodologies proposed are based on the changes of the AgNPs by two main principles: the formation of AgNPs aggregates causing a color change in the suspension [4,5] and the modification of size and shape of the AgNPs by the use of oxidizing or reducing analytes [6,7].…”

Section: Introductionmentioning

confidence: 99%

Determination of Lipoxygenase Activity in Cereals Grains Using a Silver Nanoparticles Assay

Muñoz

Rodríguez

Páez-Hernández

et al. 2015

Journal of Chemistry

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A spectrophotometric method based on the use of silver nanoparticles is presented for the determination of lipoxygenase activity in cereal grains. The method involves the synthesis of prism silver nanoparticles with a maximum absorption at 752 nm, followed by the abatement of the signal as a consequence of the oxidation produced by hydroperoxides generated in the enzymatic reaction. The quantification of the hydroperoxides produced by reaction of lipoxygenase with linoleic acid allows the determination of the enzyme activity in cereal grains. Under optimal conditions, the linear range of the calibration curve ranges from 1.0 to 5.0 M, with a limit of detection of 0.34 M. The method was validated comparing the results with those obtained by ferrous-xylenol orange method. A relative standard deviation < 5.0% was obtained in all cases and no significant differences were observed ( < 0.05).

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Lectin sensitized anisotropic silver nanoparticles for detection of some bacteria

Cited by 33 publications

References 35 publications

Biosensors for Whole-Cell Bacterial Detection

Biosensors for Whole-Cell Bacterial Detection

Development of nanoparticle-based optical sensors for pathogenic bacterial detection

Determination of Lipoxygenase Activity in Cereals Grains Using a Silver Nanoparticles Assay

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