Lectin-induced alterations on the proliferation of three human prostatic cancer cell lines

In vitro influence of lectins and neoglycoconjugates on the growth of three human sarcoma cell lines

“…However some transient, but nevertheless statistically signi®cant, lectin-induced stimulation of cell proliferation was also observed. These transient stimulatory eects at low doses have also been reported in other cancer cell lines studies (Camby et al 1996Lorea et al 1997).…”

“…Indeed, PNA lectin, which binds to galactose-b1,3-N-acetylgalactosamine, inhibits breast cancer cell proliferation (Marth and Daxenbichler 1988) while stimulating smooth muscle and pulmonary arterial cell proliferation (Sanford and Harris-Hooker 1990) as well as benign and malignant colorectal cells (Ryder et al 1994a, b). In our laboratory, previous studies undertaken on prostatic cancer (Camby et al 1996) and astrocytic cell lines ) demonstrated that the same plant lectins had a globally signi®cant dose-dependent toxic eect. Nevertheless, low doses of GSA-IA4 and PHA-L signi®cantly increased cell proliferation in one of the prostatic cancer cell lines (Camby et al 1996).…”

“…In our laboratory, previous studies undertaken on prostatic cancer (Camby et al 1996) and astrocytic cell lines ) demonstrated that the same plant lectins had a globally signi®cant dose-dependent toxic eect. Nevertheless, low doses of GSA-IA4 and PHA-L signi®cantly increased cell proliferation in one of the prostatic cancer cell lines (Camby et al 1996).…”

“…As previously described (Camby et al 1996;Janssen et al 1996), cell growth was assessed by means of the MTT assay according to Mosman (1983), but with the modi®cations recommended by Carmichael et al (1987). Brie¯y, the assessment of proliferation is based on the ability of living cells to reduce the yellow product MTT [3-(4,5)-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, Sigma] to a blue product, formazan, by a reduction reaction occurring in the mitochondria.…”

Cloning, overexpression, purification, and modeling of a lectin (Rhinocelectin) with antiproliferative activity from Tiger Milk Mushroom, Lignosus rhinocerus

“…Burgeoning evidences on lectins impeding or inhibiting cancer growth are encouraging, and provide the rational to search for anticancer lectins in rare mushrooms used in folk medicine such as the TMM L. rhinocerus . As most lectins are heat and chemical resistant, they are capable of binding and interfering with the gut interactions, shifting bacterial floral and passing into systemic circulation, with some reported toxic and inflammatory effects . Lectins account for up to 39.13% of the major soluble protein constituents of the sclerotium in L. rhinocerus .…”