Lectin, hemolysin and protease inhibitors in seed fractions with ovicidal activity against Haemonchus contortus

Salles, H. O.; Braga, Ana Carolina Linhares; Nascimento, Maria Thayana dos Santos Canuto do; Sousa, Ana Márjory Paiva; Lima, Adriano Rodrigues; Vieira, L. da S.; Cavalcante, A. C. R.; Egito, A. S. do; Andrade, L. B. da S.

doi:10.1590/s1984-29612014050

Cited by 23 publications

(9 citation statements)

References 26 publications

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“…Mashela & Nthangeni (2002) observed that castor used in soil favoured the growth of tomato, without affecting soil pH, and suppressed the nematode Meloidogyne incognita. In addition, ovicidal activity against Haemonchus contortus was already detected in fractions of the seed extracts of Ricinus communis by Salles et al (2014). This present work shows that castor cake controls the free-living stage of small ruminant nematodes in vitro and has the additional benefit of nourishing the plant.…”

Section: Discussionsupporting

confidence: 62%

Crop residues activity against the free-living stages of small ruminant nematodes

Salles

Braga

Nascimento

et al. 2019

Rev. Bras. Parasitol. Vet.

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The nematicidal effect of different organic materials was evaluated in order to develop a non-chemical alternative soil treatment for control of the free-living stages of small ruminant gastrointestinal nematodes. The selected organic materials were residues from the juice industry of acerola, cashew, grape, guava, papaya and pineapple, as well as castor residue from the biodiesel industry. LC90 results showed that pineapple residue was the most efficient inhibitor of larval development, followed by castor, grape, cashew, acerola, guava and papaya. Castor residue was also a good source of nitrogen and was used in a greenhouse experiment to prevent larval development in contaminated goat faeces that was deposited in pots containing the grasses Brachiaria brizantha (var. Paiaguás) or Megathyrsus maximus x M. infestum (var. Massai). Castor residue caused a significant (P < 0.05) reduction (85.04%) in Paiaguás grass contamination (L3.dry mass-1) and a reduction of 17.35% in Massai grass contamination (P > 0.05), with an increase in the biomass production of Massai (251.43%, P < 0.05) and Paiaguás (109.19%, P > 0.05) grasses. This strategy, called Econemat®, with good results in vitro shows to be promising on pasture increasing phytomass production.

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Section: Discussionsupporting

confidence: 62%

Crop residues activity against the free-living stages of small ruminant nematodes

Salles

Braga

Nascimento

et al. 2019

Rev. Bras. Parasitol. Vet.

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“…A previous study reported that the crude water extract of Moringa oleifera seed showed high egg hatch inhibition on Haemonchus contortus [52]. The results of this work describe a potential use of Moringa oleifera in controlling nematode parasites [53].…”

Section: Schistosomiasis Is a Chronic Tropical Disease Caused By Parasupporting

confidence: 58%

Mini Review on Antimicrobial Activity and Bioactive Compounds of Moringa oleifera

Wang¹,

Chen²,

Wu³

2016

Med chem (Los Angeles)

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Moringa oleifera is a kind of woody tree traditionally used as a nutritional source and as a medicinal plant. It grows wild in the tropical and subtropical areas of Asia, Africa and the Middle East. In China, Moringa oleifera trees are planted at a large scale in Yunnan, Guangdong and Guangxi Provinces. As a nutritional and medicinal plant, Moringa oleifera is a rich source of bioactive compounds with diverse pharmacological activities. It has been widely used in the treatment of certain diseases as a traditional medicinal herb. Antimicrobial activity is the most studied property of Moringa oleifera. Many studies have shown that the leaf, flower, bark, root, seed, and nearly all types of Moringa oleifera tissues exhibit antimicrobial activity including antibacterial, antifungal, antiviral and antiparasitic activity. This review describes progress on research conducted to understand the antimicrobial activity and related bioactive properties of Moringa oleifera compounds, and discusses the potential use of Moringa oleifera in the control of pathogenic microbes.

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“…Although secondary metabolites have been extensively correlated with nematicidal effects, demonstrating their applicability to controlling the gastrointestinal nematodes of small ruminants, plant proteins represent a novel approach for controlling these parasites (SALLES et al, 2014). The use of therapeutic proteins is one of the fastest growing sectors in the pharmaceutical market and has undergone several generations of development to achieve increasingly viable commercial products.…”

Section: Discussionmentioning

confidence: 99%

Anthelmintic activity of Leucaena leucocephala protein extracts on Haemonchus contortus

Soares

Araújo

Lopes

et al. 2015

Rev. Bras. Parasitol. Vet.

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The objective of this study was to evaluate the effects of protein extracts obtained from the plant Leucaena leucocephala on the nematode parasite Haemonchus contortus. The seeds, shell and cotyledon of L. leucocephala were separated and their proteins extracted using a sodium phosphate buffer, and named as TE (total seed extract), SE (shell extract) and CE (cotyledon extract). Soluble protein content, protease, protease inhibitory and chitinase activity assays were performed. Exsheathment inhibition of H. contortus larvae were performed at concentrations of 0.6 mg mL . The effective concentration for 50% hatching inhibition (EC 50 ) was estimated by probit. Different proportions of soluble proteins, protease and chitinase were found in TE and CE. Protease inhibitory activity was detected in all extracts. The EC 50 of the CE and TE extracts were 0.48 and 0.33 mg mL -1 , respectively. No ovicidal effects on H. contortus were detected in SE extracts, and none of the protein extracts demonstrated larvicidal effects on H. contortus. We therefore conclude that protein extracts of L. leucocephala had a detrimental effect on nematode eggs, which can be correlated with the high protease and chitinase activity of these extracts.Keywords: Plant proteins, protease, protease inhibitor, chitinase. ResumoO objetivo deste estudo foi avaliar a atividade anti-helmíntica de extratos proteicos de leucena (Leucaena leucocephala) sobre Haemonchus contortus. As sementes, as cascas e os cotilédones foram moídos separadamente e as proteínas extraídas com tampão fosfato de sódio e denominados: TE (extrato total), SE (extrato casca) e CE (extrato cotilédone). O teor de proteínas, atividade proteolítica, inibitória de protease e quitinolítica dos extratos foram verificados, além da ação sobre a eclosão de ovos e desembainhamento larvar de H. contortus. A concentração efetiva para inibição de 50% da eclosão dos ovos (EC 50 ) foi calculada através do probit. Foi demonstrado que TE e CE possuem, em diferentes proporções, proteínas solúveis, protease e quitinase. Atividade inibitória de protease foi encontrada em todos os extratos. A EC 50 dos extratos CE e TE foram 0,48 e 0,33 mg de proteína mL -1 , respectivamente. O extrato SE não apresentou atividade sobre a eclosão dos ovos. Os extratos proteicos não apresentaram efeito larvicida sobre H. contortus. Conclui-se que a ação de extratos proteicos de L. leucocephala afetam negativamente a eclodibilidade dos ovos, correlacionando-se com a alta atividade de protease e quitinase dos extratos testados.Palavras-chaves: Proteínas vegetais, protease, inibidor de protease, quitinase.

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Lectin, hemolysin and protease inhibitors in seed fractions with ovicidal activity against Haemonchus contortus

Cited by 23 publications

References 26 publications

Crop residues activity against the free-living stages of small ruminant nematodes

Crop residues activity against the free-living stages of small ruminant nematodes

Mini Review on Antimicrobial Activity and Bioactive Compounds of Moringa oleifera

Anthelmintic activity of Leucaena leucocephala protein extracts on Haemonchus contortus

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