Lectin from seeds of a Brazilian lima bean variety (Phaseolus lunatus L. var. cascavel) presents antioxidant, antitumour and gastroprotective activities

Lacerda, Rodrigo Rodrigues e; Nascimento, Edilza Silva do; Lacerda, José Thalles Jocelino Gomes de; Pinto, Luciano da Silva; Rizzi, Caroline; Bezerra, Mirna Marques; Pinto, Isabela Ribeiro; Filho, Samuel Mateus Pereira; Pinto, Vicente de Paulo Texeira; Filho, Gerardo Cristino; Gadelha, Carlos Alberto de Almeida; Santi-Gadelha, Tatiane

doi:10.1016/j.ijbiomac.2016.10.097

Cited by 36 publications

(25 citation statements)

References 72 publications

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“…C. arietinum L. is the mere species that is developed for cultivation and/or came into existence from its wild counterpart C. reticulatum (Ladizinsky and Alder, ). This is contradictory to earlier published reports where in lectins from same species differ in their molecular weights endorsing differences that exist between proteins present in various seed types of the same species (Lacerda et al, ). Published reports state that chickpea lectin show diversity in molecular weight from 35 to 120 kDa (Gautam et al, ; Kolberg, Michaelsen, & Sletten, ; Qureshi, Dash, Srivastava, & Koundal, ).…”

Section: Discussioncontrasting

confidence: 99%

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Biochemical characterisation of lectin from wild chickpea (Cicer reticulatumL.) with potential inhibitory action against human cancer cells

2018

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A wild chickpea lectin (WCL) from the seeds of Cicer reticulatum L. was chromato‐purified using DEAE‐Cellulose and SP‐Sephadex ion exchange chromatography. WCL was thermostable upto 60°C with broad pH optima (pH 5–9) and various divalent metal ions did not influence its activity. WCL demonstrated DNA protection in a dose‐dependent manner. The lectin exerted antifungal activity against diverse fungal pathogens. WCL augmented the mitogenic response of mouse spleen cells at 10 µg/ml concentration and showed an inhibition of HIV‐1 reverse transcriptase at IC50 of 200 µM. Against human cancer cell lines, lectin demonstrated anticancer potential. The cell viability assay in HepG2, Ishikawa, MCF‐7 and MDA‐MB‐231 cell line demonstrated IC50 values of 61.8, 54.4, 37.5 and 44.2 µg/ml respectively. Practical applications WCL exhibited distinct medicinal properties vis‐à‐vis antiproliferative, mitogenic, antifungal/bacterial and HIV‐1 reverse transcriptase inhibiting activities. The potential of WCL can be a subject of exploration from a pharmacological standpoint.

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Section: Discussioncontrasting

confidence: 99%

“…No differences in molecular weights of cultivated and wild chickpea was observed and are in agreement with earlier published reports on other crop plants (Lacerda, ). Recently Gautam et al () isolated lectin from C. arietinum L. seeds (cv.…”

Section: Discussionsupporting

confidence: 92%

Biochemical characterisation of lectin from wild chickpea (Cicer reticulatumL.) with potential inhibitory action against human cancer cells

2018

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“…1) presented three distinct protein peaks (GO1, GO2, GO3), where only the third peak (GO3) displayed hemagglutinating activity against rabbit erythrocyte. Similar purification procedures were employed by Galbraith and Goldstein [35] and eLacerda et al [36]. In both studies, ammonium sulphate precipitation of the protein preceded size exclusion chromatography on Sephadex G-200 and Sephadex G-100, respectively.…”

Section: Resultsmentioning

confidence: 93%

In-vitro Antioxidant and Antibacterial Potential of Mannose/Glucose-binding Pterocarpus osun Craib. Seeds Lectin

Odekanyin

Akande

2019

JALSI

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Objective: This study was carried out to purify and characterize a carbohydrate-binding and cell-agglutinating protein, lectin, from Pterocarpus osun seeds and also to evaluate its antioxidant and antibacterial potential. Methods: Isolation and purification of the lectin were done by ammonium sulphate precipitation and size exclusion chromatography on Sephadex G-100. Physicochemical properties of the lectin were determined and antioxidant activity was evaluated by DPPH radical scavenging, lipid peroxidation inhibition and ferric reducing antioxidant potential assay. A disc diffusion method was used for antibacterial effect. Results: Lectin was detected in the seeds and was able to agglutinate native and enzyme-treated rabbit erythrocytes but only enzyme-treated erythrocytes of human blood were agglutinated. Mannose, Maltose and α-methylmannoside inhibited the divalent cation-independent hemagglutinating activity, which was stable up to 60°C and at pH range of 3-13. It showed antioxidant activity with IC50 of 1.17 ± 0.08, 0.58 ± 0.03, and 2.51 ± 0.03 mg/ml for those methods respectively. No antibacterial activity was observed. Conclusion: Pterocarpus osun seeds lectin possess properties similar to other lectins from Dalbergieae tribe and its ability to scavenge free radical and inhibit lipid peroxidation show the presence of a valuable health promoting agent in the seeds.

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“…They possess the ability to induce agglutination of cells and have been explored for their biomedical potential [19]. They manifest diverse biological activities such as mitogenic [20][21][22], antiproliferative [23,24], immunomodulatory [25,26], antitumor [27], antimicrobial [28,29], antioxidant [30,31], etc.…”

Section: Introductionmentioning

confidence: 99%

“…There has been an increase in research into new compounds with antioxidant activity in natural products. Many plant lectins have been reported to possess an antioxidant potential [23,27,28]. However, there is only one report on the antioxidant potential of lectins from microfungi [33].…”

Section: Introductionmentioning

confidence: 99%

Antioxidant and antimicrobial activities of Penicillium sp. lectins

Singh

Walia

2019

ARCH BIOL SCI BELGRA

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Lectins are a diverse group of proteins of non-immune origin that interact specifically with glycans. Owing to their specificity, they can mediate various cellular and molecular recognition processes. To explore information on biological activities of lectins from Penicillium duclauxii, P. proteolyticum and P. griseoroseum, they were investigated for their antioxidant and antimicrobial activities. Penicillium sp. lectins exhibited moderate antioxidant activity. P. duclauxii, P. proteolyticum and P. griseoroseum lectins inhibited DPPH with an IC 50 value of 71.42, 75.04 and 82.11 µg/mL, respectively. P. duclauxii, P. proteolyticum and P. griseoroseum lectins inhibited the hydrogen peroxide radical with IC 50 values of 198.57, 209.76 and 215.31 µg/mL, respectively. P. duclauxii and P. proteolyticum lectins exhibited potent antibacterial activity against Gram-negative bacteria. P. griseoroseum lectin inhibited only Gram-positive bacteria. Penicillium sp. lectins did not exhibit antifungal activity. The biological potential of Penicillium sp. lectins will help to understand their biomedical applications. This is the first report on the antioxidant and antimicrobial activities of purified lectins from Penicillium sp.

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Lectin from seeds of a Brazilian lima bean variety (Phaseolus lunatus L. var. cascavel) presents antioxidant, antitumour and gastroprotective activities

Cited by 36 publications

References 72 publications

Biochemical characterisation of lectin from wild chickpea (Cicer reticulatumL.) with potential inhibitory action against human cancer cells

Biochemical characterisation of lectin from wild chickpea (Cicer reticulatumL.) with potential inhibitory action against human cancer cells

In-vitro Antioxidant and Antibacterial Potential of Mannose/Glucose-binding Pterocarpus osun Craib. Seeds Lectin

Antioxidant and antimicrobial activities of Penicillium sp. lectins

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