Lectin from Laetiporus sulphureus effectively inhibits angiogenesis and tumor development in the zebrafish xenograft models of colorectal carcinoma and melanoma

Petrović, Jovana; Glamočlija, Jasmina; Ilić-Tomič, Tatjana; Soković, Marina; Robajac, Dragana; Nedić, Olgica; Pavić, Aleksandar

doi:10.1016/j.ijbiomac.2020.01.033

Cited by 27 publications

(15 citation statements)

References 44 publications

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“…In addition, the scalable nature of this assay with potential 96-well format screening makes it a potential platform for the identification of new drugs through chemical compound screens (Figure 1). Several recent studies use zebrafish xenografts for in vivo validation of drug efficacy, either alone or as a complementary in vivo assay to mouse xenografts [29,35,[59][60][61][62][63][64][65]. In addition to validating drug efficacy, zebrafish xenografts have validated the effectiveness of drug delivery platforms, particularly for nanomedicine formulations [66].…”

Section: Zebrafish Xenografts Enable Drug Discovery Validation and Screeningmentioning

confidence: 99%

Zebrafish Xenografts for Drug Discovery and Personalized Medicine

2020

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Cancer is the second leading cause of death in the world. Given that cancer is a highly individualized disease, predicting the best chemotherapeutic treatment for individual patients can be difficult. Ex vivo models such as mouse patientderived xenografts (PDX) and organoids are being developed to predict patient-specific chemosensitivity profiles before treatment in the clinic. Although promising, these models have significant disadvantages including long growth times that introduce genetic and epigenetic changes to the tumor. The zebrafish xenograft assay is ideal for personalized medicine. Imaging of the small, transparent fry is unparalleled among vertebrate organisms. In addition, the speed (5-7 days) and small patient tissue requirements (100-200 cells per animal) are unique features of the zebrafish xenograft model that enable patient-specific chemosensitivity analyses. Zebrafish Have Become a Leading Animal Model for Human Disease and Personalized MedicineFifty years ago, George Streisinger took a leap of faith. He chose a fish for his vertebrate genetic model organism (see Glossary). Many of his contemporaries believed that a fish would be too evolutionarily distant from mammals to have any relevance for human health, and that he would only learn about the genetics of fish [1]. We have since learned that zebrafish and humans have more in common than not. For example, zebrafish retain most of the same organs ('two eyes, mouth, brain, spinal cord, intestine, pancreas, liver, bile ducts, kidney, esophagus, heart, ear, nose, muscle, blood, bone, cartilage, and teeth') but notably lack lungs. Zebrafish would therefore be unsuitable for the development of lung disease models (although they would make an excellent lung cancer metastasis model) [2]. Publication of the complete zebrafish genome in 2013 showed that 70% of the genes in zebrafish have human orthologs, and cross-comparison of disease-related genes annotated in the Online Mendelian Inheritance in Man (OMIM) database reveals that 82% of those genes have at least one zebrafish ortholog [2,3]. HighlightsZebrafish xenografts are proven models of human cancer biology that provide rapid in vivo validation of anticancer drugs and drug targets.Zebrafish have emerged as unsurpassed models for imaging tumor metastasis.

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Section: Zebrafish Xenografts Enable Drug Discovery Validation and Screeningmentioning

confidence: 99%

Zebrafish Xenografts for Drug Discovery and Personalized Medicine

2020

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“…In this study, the effects of casearlucin A on zebrafish intersegmental vessels formation were examined. Herein, we used the transgenic zebrafish embryos to directly visualize the effect of casearlucin A on the new vessel development (37,38). As shown in Figure 8, in contrast with the blank control, the intersegmental vessels (ISVs) and dorsal longitudinal anastomotic vessels (DLAVs) were absent and broken after treatment with casearlucin A and the positive control, sunitinib malate.…”

Section: Antiangiogenetic Activity Of Casearlucin a Using A Transgenic Zebrafish Modelmentioning

confidence: 99%

The Antitumor Activity and Mechanism of a Natural Diterpenoid From Casearia graveolens

Liu

Song

et al. 2021

Front. Oncol.

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Casearlucin A, a diterpenoid obtained from Casearia graveolens, has been reported to possess strong cytotoxic activity. However, the in vivo anti-tumor effects and the action mechanism of casearlucin A remain poorly understood. Our study revealed that casearlucin A arrested cell cycle at G0/G1 stage and induced cell apoptosis in cell level. Additionally, casearlucin A inhibited HepG2 cell migration via regulating a few of metastasis-related proteins. Furthermore, it inhibited tumor angiogenesis in zebrafish in vivo. More importantly, casearlucin A significantly inhibited cell proliferation and migration in an in vivo zebrafish xenograft model. Collectively, these results are valuable for the further development and application of casearlucin A as an anticancer agent.

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“…Agrocybe aegerita and Polyporus squamosus were collected from popular trees at Jabučki rit (northern Serbia) during April 2020 and authenticated by Jasmina Glamočlija (Institute for Biological Research, the University of Belgrade, Serbia). Samples were prepared, as previously described Petrovic et al, 2020 [21]. A voucher specimen was deposited at the Fungal Collection Unit of the Mycological Laboratory, Department of Plant Physiology, Institute of Biological Research "Siniša Stanković", Belgrade, the Republic of Serbia, under the number Ls-09-2020, Asyl-001-2020, Po-09-2020, Aa-04-2020 and Ps-04-2020.…”

Section: Collecting and Preparation Of Ethanol Extracts Of Fruiting Bodiesmentioning

confidence: 99%

“…Toxicity evaluation of fungal ethanol extracts was carried in the zebrafish (Danio rerio) model, according to previously published procedure [21,22] following the general rules of the OECD Guidelines for the Testing of Chemicals (OECD, 2013, Test No. 236) [23].…”

Section: In Vivo Toxicity Assessment In the Zebrafish Modelmentioning

confidence: 99%

“…Bearing in mind an exceptional medicinal importance of macrofungi, Glamoclija and collaborators conducted the extensive studies on biological activities of edible and medicinal mushrooms, pointing them out as a rich resource of bioactive molecules with antibacterial, antifungal [19], antioxidative [20], antiangiogenic, and anticancer [21] properties. In this study, we have explored the depigmenting potential of the ethanol extract of five edible mushrooms (Lethiporus sulphureus, Agaricus silvaticus, Agrocybe aegerita, Pleurotus ostreatus, Polyporus squamosus) in vivo and comprehensively assessed their possibility to be applied as topical depigmenting agents.…”

Section: Introductionmentioning

confidence: 99%

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Unravelling Anti-Melanogenic Potency of Edible Mushrooms Laetiporus sulphureus and Agaricus silvaticus In Vivo Using the Zebrafish Model

Pavić

Ilić-Tomič

Glamočlija

2021

JoF

Self Cite

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Severe drawbacks associated with the topical use of depigmenting agents in treatments of skin hyperigmentations impose a great demand for novel, effective, and safe melanogenesis inhibitors. Edible and medicinal mushrooms, known for numerous health-promoting properties, represent a rich reservoir of anti-melanogenic compounds, with the potential to be applied in preventing excessive skin pigmentation. Herein, using zebrafish (Danio rerio) as a preclinical animal model, we have demonstrated that ethanol extract of Laetiporus sulphureus (LSE) and Agaricus silvaticus (ASE) are not toxic at high doses up to 400–500 µg/mL while effectively inhibit melanogenesis in a dose-dependent manner. At depigmenting doses, the explored extracts showed no adverse effects on zebrafish embryos melanocytes. Even more, they did not provoke inflammation or neutropenia when applied at the highest dose ensuring almost complete the cells depigmentation. Since LSE and ASE have demonstrated significantly higher the therapeutic potential than kojic acid and hydroquinone, two well-known depigmenting agents, overall results of this study strongly suggest that the explored mushrooms extracts could be used as efficient and safe topical agents in treatments of skin hyperpigmentation disorders.

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Lectin from Laetiporus sulphureus effectively inhibits angiogenesis and tumor development in the zebrafish xenograft models of colorectal carcinoma and melanoma

Cited by 27 publications

References 44 publications

Zebrafish Xenografts for Drug Discovery and Personalized Medicine

Zebrafish Xenografts for Drug Discovery and Personalized Medicine

The Antitumor Activity and Mechanism of a Natural Diterpenoid From Casearia graveolens

Unravelling Anti-Melanogenic Potency of Edible Mushrooms Laetiporus sulphureus and Agaricus silvaticus In Vivo Using the Zebrafish Model

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