Lectin cytochemistry in the gastrointestinal tract with special reference to glycosylation in the Golgi apparatus of Brunner's gland cells.

Suzaki, Etsuko; Kataoka, Kazunori

doi:10.1177/40.3.1552177

Cited by 31 publications

(26 citation statements)

References 18 publications

(28 reference statements)

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“…This conclusion is based on the fact that three mucous neck cell markers (TFF2/GSII/MUC6, as shown here and previously [26][27][28][29] ), but not Cdx2, labeled this expanded compartment in both animal models. Previously, gastric mucosal changes with histological features of Brunner's gland cells were designated as metaplastic.…”

Section: Discussionsupporting

confidence: 79%

Interferon gamma induction of gastric mucous neck cell hypertrophy

Kang

Rathinavelu

Samuelson

et al. 2005

Laboratory Investigation

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Chronic inflammation of the gastric epithelium is believed to induce mucosal changes that can eventually develop into gastric cancer. In gastrin-deficient (GÀ/À) mice exhibiting chronic inflammation in the hypochlorhydric stomach, we documented a prominent fundic mucous cell lineage sharing morphological similarity with preneoplastic changes reported in Helicobacter-infected mice. To study the identity and origin of this cell lineage, we screened for different gastric mucosal cell markers. The clusters of large, foamy cells stained for trefoil factor 2 (TFF2/SP), MUC6 and the lectin Griffonia Simplicifolia II (GSII), but not for the intestine-specific transcription factor Cdx2, suggested that they arise from gastric mucous neck cells. Ki67-labeled GSII-positive neck cells in Helicobacter felis-infected, but not GÀ/À stomachs, suggested that mucous neck cell proliferation accounted for expansion of this compartment in the H. felis model of gastritis, but not the GÀ/À model. Using RNase protection assays and quantitative PCR, we found that interferon gamma (IFNc) was the most abundant proinflammatory cytokine in the GÀ/À stomach. We also found that this Th1 cytokine can increase the abundance of mucous neck cells, since its infusion into mice recapitulated the appearance of these cells as observed in both GÀ/À and H. felis-infected mice. Using the human gastric cell line NCI-N87, we showed that IFNc induces the secretion of mucus and expression of MUC6, TFF2 and pepsinogen II, but not of pepsinogen I and intrinsic factor. In conclusion, our results demonstrate that inflammation, specifically the proinflammatory cytokine IFNc, induced expansion of the fundic mucous neck cell compartment, which likely represents both increased mucus production and cell number.

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Section: Discussionsupporting

confidence: 79%

Interferon gamma induction of gastric mucous neck cell hypertrophy

Kang

Rathinavelu

Samuelson

et al. 2005

Laboratory Investigation

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“…Immunoelectron microscopy was performed essentially as described by Suzuki and Kataoka (1992) and Tomoyasu et al (1993b), except that the leaves were cut into 1 ϫ 1-mm pieces and fixed with 0.1% glutaraldehyde and 4% paraformaldehyde in sodium cacodylate, pH 7.4, under vacuum conditions. The tissues were embedded in London Resin White (London Resin Co., London, UK) at Ϫ20ЊC.…”

Section: Immunoelectron Microscopymentioning

confidence: 99%

Reduced Levels of Chloroplast FtsH Protein in Tobacco Mosaic Virus–Infected Tobacco Leaves Accelerate the Hypersensitive Reaction

et al. 2000

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In tobacco cultivars resistant to tobacco mosaic virus (TMV), infection results in the death of the infected cells accompanying the formation of necrotic lesions. To identify the genes involved in this hypersensitive reaction, we isolated the cDNA of tobacco DS9, the transcript of which decreases before the appearance of necrotic lesions. The DS9 gene encodes a chloroplastic homolog of bacterial FtsH protein, which serves to maintain quality control of some cytoplasmic and membrane proteins. A large quantity of DS9 protein was found in healthy leaves, whereas the quantity of DS9 protein in infected leaves decreased before the lesions appeared. In transgenic tobacco plants containing less and more DS9 protein than wild-type plants, the necrotic lesions induced by TMV were smaller and larger, respectively, than those on wild-type plants. These results suggest that a decrease in the level of DS9 protein in TMV-infected cells, resulting in a subsequent loss of function of the chloroplasts, accelerates the hypersensitive reaction. INTRODUCTIONThe hypersensitive reaction (HR) is a plant defense system against pathogen attack. HR is defined as the rapid death of infected cells accompanying formation of necrotic lesions in which pathogens are thought to be enclosed (Goodman and Novacky, 1994). The HR of tobacco to tobacco mosaic virus (TMV) occurs in cultivars carrying the resistance gene N ( NN tobacco) but not in cultivars lacking the N gene ( nn tobacco) (Holmes, 1938; Whitham et al., 1994). Induction of the N gene-mediated HR requires the temperature to be below 22 to 26 Њ C (Kassanis, 1952;Shimomura, 1971; Weststeijn, 1981), suggesting that the gene can function at temperatures below this range.We previously observed that when TMV-inoculated leaves were treated with heat shock or actinomycin D, necrotic lesions were induced in not only NN but also in nn tobacco, even at 30 Њ C, a nonpermissive temperature for the N gene (Shimomura and Ohashi, 1971;Ohashi and Shimomura, 1972). In the actinomycin D-treated leaves in which necrotic lesions were induced, the synthesis of nucleic acid in the host plant was greatly suppressed, whereas the synthesis of TMV RNA was suppressed only slightly (Shimomura and Ohashi, 1980). In contrast to treatment with actinomycin D, treatment with other chemicals, including rifampicin, 2-thiouracil, and cycloheximide, which suppress RNA or protein synthesis in both the TMV and the host plant, did not induce necrotic lesions to form (Ohashi and Shimomura, 1972). Given that actinomycin D forms intercalation complexes with DNA, thereby blocking transcription (Waring, 1981), and that heat shock suppresses transcription of housekeeping genes (Lindquist, 1986;Nover et al., 1989), we hypothesized that suppressing the transcription of certain housekeeping genes in NN tobacco plants after infection with TMV could induce the HR.To isolate such candidate genes, we used a synchronous HR-inducing system based on a temperature shift. Incubation at 30 Њ C of TMV-infected NN tobacco leaves results in 1 Curr...

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“…After 5 rain, the ceils were harvested and resuspended in the same fixative solution buffered with 50 mM sodium eacodylate (pH 7.2) for 1 h. The cells were dehydrated with an ethanol series and embedded in London Resin white (Suzaki et al 1992). Ultrathin sections were mounted on uncoated gold grids and used for immunostaining and in situ hybridization.…”

Section: Sample Preparation For Electron Microscopymentioning

confidence: 99%

Assembly of plasmid DNA and chromatophore inRhodospirillum rubrum

et al. 2000

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Summary. Rhodospirillurn rubrum, a photosynthetic bacterium, contains many photosynthetic vesicular membranous structures called chromatophores. The organism contains a 55 kb specific plasmid which is essential for photosynthesis, but the exact relationship between the chromatophore and the plasmid is uncertain. In this study we examined the precise localization of the plasmids, especially in relation to the chromatophores. Fluorescence in situ hybridization indicated that there are several copies of the plasmid per cell and that some plasmids are localized close to the cellular envelope. In situ hybridization at the electron-microscopic level further revealed that the plasmid localized to the periphery of the chromatophore close to the envelope. Moreover, when the chromatophore fraction was purified from cells, the plasmid DNA was observed as a cluster around the chromatophore vesicles. The assembly of the plasmid and chromatophore may be related to chromatophore formation by invagination of cell membrane.

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Lectin cytochemistry in the gastrointestinal tract with special reference to glycosylation in the Golgi apparatus of Brunner's gland cells.

Cited by 31 publications

References 18 publications

Interferon gamma induction of gastric mucous neck cell hypertrophy

Interferon gamma induction of gastric mucous neck cell hypertrophy

Reduced Levels of Chloroplast FtsH Protein in Tobacco Mosaic Virus–Infected Tobacco Leaves Accelerate the Hypersensitive Reaction

Assembly of plasmid DNA and chromatophore inRhodospirillum rubrum

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