Lectin binding patterns of uterine glands in mares with chronic endometrial degeneration

Walter, Ingrid; Klein, Michaela; Handler, Johannes; Aurich, Jörg; Reifinger, M.; Aurich, Christine

doi:10.2460/ajvr.2001.62.840

Cited by 23 publications

(24 citation statements)

References 11 publications

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“…This is in agreement with other investigations reporting morphological and functional features in endometrotic glands that deviate from those of adjacent non-altered glands (Stewart et al 2000, Walter et al 2001, Lehmann et al 2011). The aberrant expression of components of the uterine milk (e.g.…”

Section: Discussionsupporting

confidence: 83%

“…The observed BD immunolabelling pattern together with findings of other investigations suggests functional differences between the different epithelial cell populations of the equine endometrium (Gerstenberg et al 1999, Walter et al 2001, Hoffmann et al 2009b. In regard to the synthesis of BD protein, this function seems to be performed mainly by endometrial epithelial cells within a superficial location (luminal surface and gland ducts).…”

Section: Discussionmentioning

confidence: 71%

“…The failure of glandular epithelial cells to produce significant amounts of the antimicrobial protein BD may predispose to persistent bacterial infections of glan- dular lumina. Using lectin histochemistry, a similar glycoprotein expression pattern is detected within luminal and ductular epithelium that differs from those of epithelial cells of remaining glandular portions (Walter et al 2001). Uteroglobin, uteroferrin and glycogen are predominantly detected within the glandular epithelium (mid and basal glands), whereas uterocalin is mainly localized within the surface epithelium (Hoffmann et al 2009b).…”

Section: Discussionmentioning

confidence: 76%

“…a cytoplasmic expression of BD protein (this study) and/or specific glycoproteins (Walter et al 2001). Thus, endometrosis seems not only to disturb specific glandular functions, but may also impair the spatial organisation of cellular functions within the equine endometrium.…”

Section: Discussionmentioning

confidence: 99%

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New insights into the innate immune defences of the equine endometrium: in situ and in vitro expression pattern of beta-defensin

Schöniger¹,

Böttcher²,

Theuß³

et al. 2016

PHK

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Summary: Equine endometrial diseases are a frequent cause of subfertility causing financial loss. Degenerative and inflammatory diseases are often associated with alterations of the innate immunity and beta-defensin has been identified within the equine endometrium. The aim of this study was to further characterize the expression of beta-defensin protein within in the equine endometrium by immunohistochemistry. For in situ investigations, endometrial samples were collected from 26 mares once at different stages of the oestrus cycle and from 3 mares repeatedly during the same oestrus cycle (days 0, 5, 10, 13, 16, 19 and 21; day 0 is defined as ovulation day). In vitro examinations were performed on cultured epithelial cells obtained from endometrial specimens of 15 mares. The following results were obtained: Endometrial tissue sections of the 26 mares were either without pathological alterations (n = 3) or showed varying degrees of endometritis, endometrosis and/or angiosclerosis. Beta-defensin protein was mainly expressed within the cytoplasm of epithelial cells lining the luminal surface (100 %, 26 mares) and glandular ducts (69 %, 18 mares) and rarely within the nucleus of these cell populations (8 % and 19 %, respectively). The vast majority of cells showed either a cytoplasmic or a nuclear immunostaining. A simultaneous cytoplasmic and nuclear immunoreaction within the same cell was seldom observed (3 mares, up to 1% of the analyzed cells). Mid and basal glands without endometrosis were rarely immunopositive (nuclear immunostaining: 5 mares; cytoplasmic labelling: 1 mare). In contrast, endometrotic glands often contained small to moderate numbers of epithelial cells with a cytoplasmic immunosignal (15 of 19 cases; 79 %). In regard to the repeatedly collected biopsies of three mares, the percentage of immunopositive cells and the calculated immunoreactive scores were highly variable over the course of the oestrus cycle and between individual mares. In regard to cultured endometrial epithelial cells (n =15 mares), a positive beta-defensin immunosignal was observed in almost all cells (97 % of 521 analyzed cells) and was mainly located solely within the cytoplasm (76% of the analyzed cells), less frequently within the cytoplasm and nucleus (17 %) and rarely only within the nucleus (4 %). These results lead to the following conclusions: the antimicrobial peptide beta-defensin can be detected within the equine endometrium, the almost complete lack of beta-defensin immunostaining of unaltered glands could predispose to bacterial colonization of glandular lumina, the frequent beta-defensin immunoreaction of endometrotic glands indicates their functional alteration also in regard to the synthesis of beta-defensin, the variations in regard to the beta-defensin immunostaining between individual mares suggest a complex regulation of the beta-defensin expression, possibly under the influence of genetic factors, the beta-defensin immunostaining of cultured epithelial cells is similar to the in situ situation and therefor...

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Section: Discussionsupporting

confidence: 83%

Section: Discussionmentioning

confidence: 71%

Section: Discussionmentioning

confidence: 76%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

New insights into the innate immune defences of the equine endometrium: in situ and in vitro expression pattern of beta-defensin

Schöniger¹,

Böttcher²,

Theuß³

et al. 2016

PHK

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show abstract

“…Die chronisch degenerativen Veränderungen nehmen bei älteren Stuten zu (z.B. Doig et al 1981, Schoon et al 1997, was sich auch in der vorliegenden Studie bestätigt, wo das Endometrium insbesondere von Stuten ab einem Alter von 13 Jahren und mehr vermehrt in die Kategorien IIB und III nach Kenney und Doig (1986) Veränderungen des Endometriums verändert (Walter et al 2001b, Hoffmann et al 2009 …”

Section: Zusammenfassungunclassified