Lectin Binding by Crustacean Cuticle: The Cuticle of Callinectes sapidus Throughout the Molt Cycle, and the Intermolt Cuticle of Procambarus clarkii and Ocypode quadrata

Marlowe, R. L.; Dillaman, Richard M.; Roer, Robert D.

doi:10.2307/1548904

Cited by 22 publications

(18 citation statements)

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“…It is not clear for which organisms this is true and it was certainly not seen in Callinectes sapidus. While there have been several recent studies of the carbohydrate composi- tion of calcified cuticle proteins (Marlow et al, 1994;Shafer et al, 1994Shafer et al, , 1995, there have been no studies to date that have examined the carbohydrate fraction of the arthrodial membrane. Since Anderson (1998Anderson ( , 1999 has observed very little overlap in protein distribution between the two types of cuticle, there is no reason to assume that there should be many similarities between the carbohydrate moieties.…”

Section: Discussionmentioning

confidence: 99%

Formation of the arthrodial membrane in the blue crab, Callinectes sapidus

Williams

Dillaman

Elliott

2003

Journal of Morphology

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In this study the pattern of arthrodial membrane deposition in Callinectes sapidus was determined by histological and ultrastructural examination of tissues from the carpus joint of the cheliped collected during premolt, ecdysis, postmolt, and intermolt. Apolysis in the arthrodial membrane occurs at stage D(0) and is synchronous with apolysis of the calcified cuticle. Epicuticle formation begins at early stage D(1) and is completed in late stage D(1). Procuticle deposition starts at D(2) and continues until ecdysis. Numerous cytoplasmic extensions occur throughout the lamellae. Component fibers of the arthrodial membrane are intimately associated with dense plaques on the apical membrane of the underlying hypodermal cells, suggesting a site for fiber polymerization. Deposition of the arthrodial membrane continues after ecdysis, with most of the cuticle thickening occurring during stage C. When stained with PAS and counterstained with hematoxylin, a difference can be discerned between preecdysial and postecdysial procuticle of the arthrodial membrane, a distinction not made in previous studies. The boundary between the arthrodial membrane and calcified cuticle is thicker than either of the two layers and the layers overlap rather than butting up against one another. This pattern suggests that underlying hypodermal cells have to produce multiple types of cuticle over the molt cycle. A summary of the various molting patterns in C. sapidus suggests that the control of these diverse events may prove to be complex.

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Section: Discussionmentioning

confidence: 99%

Formation of the arthrodial membrane in the blue crab, Callinectes sapidus

Williams

Dillaman

Elliott

2003

Journal of Morphology

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“…Previous studies (Shafer et al 1994(Shafer et al , 1995Marlowe et al 1994) have shown that the shell of the blue crab contains nitrogen-bearing biochemicals such as chitin, proteins, and glycoproteins. These ®ndings led to the hypothesis that acidi®cation might aect the nitrogen isotope composition of the whole crab.…”

Section: Pre-analysis Sample Treatmentmentioning

confidence: 99%

A food web analysis of the juvenile blue crab, Callinectes sapidus , using stable isotopes in whole animals and individual amino acids

Fantle¹,

et al. 1999

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The stable isotope compositions (C and N) of plants and animals of a marsh dominated by Spartina alterniflora in the Delaware Estuary were determined. The study focused on the juvenile stage of the Atlantic blue crab, Callinectes sapidus, and the importance of marsh-derived diets in supporting growth during this stage. Laboratory growth experiments and field data indicated that early juvenile blue crabs living in the Delaware Bay habitat fed primarily on zooplankton, while marsh-dwelling crabs, which were enriched in C relative to bay juveniles, utilized marsh-derived carbon for growth. In laboratory experiments, the degree to which juvenile blue crabs isotopically fractionated dietary nitrogen, as well as the growth rate, depended on the protein quality of the diet. The range of δC of amino acids in laboratory-reared crabs and their diets was almost 20‰, similar to the isotopic range of amino acids of other organisms. In laboratory studies, the δC of nonessential and essential amino acids in the diet were compared to those in juvenile crabs. Isotopic fractionation at the molecular level depended on diet quality and the crabs' physiological requirements. Comparison of whole-animal isotope data with individual amino acid C isotope measurements of wild juvenile blue crabs from the bay and marsh suggested a different source of total dietary carbon, yet a shared protein component, such as zooplankton.

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“…Ruthenium red staining of the IPS in Carcinus maenas indicated that they contain glycosaminoglycans with many anionic groups, thereby enabling the IPS to bind calcium ions (Giraud-Guille, 1984a). Fluorescentlabeled lectin staining of Callinectes sapidus, Procambarus clarkii, and Ocypode quadrata was used to further describe the glycoprotein components of the cuticle (Marlowe et al, 1994). Some lectins, such as Jacalin and Concanavalin A, did not bind to the IPS, but only to the prismatic spaces (i.e., the areas bounded by the IPS), indicating qualitative differences in the carbohydrate content of the two regions.…”

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Early pattern of calcification in the dorsal carapace of the blue crab,Callinectes sapidus

2005

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The pattern of calcium carbonate deposition was observed in the dorsal carapace of premolt (D2-D3) and early postmolt (0-48 h) blue crabs, Callinectes sapidus, using scanning (SEM) and transmission (TEM) electron microscopy. Samples of dorsal carapace for SEM were quick-frozen in liquid nitrogen, subsequently lyophilized, and viewed using secondary and backscattered electrons as well as X-ray maps of calcium. Pieces of lyophilized cuticle were also embedded in epoxy resin and subsequently sectioned and viewed with TEM and SEM. Fresh pieces of dorsal carapace for TEM were also fixed in 2.5% glutaraldehyde in phosphate buffer followed by postfixation in 1% OsO4 in cacodylate buffer. Calcium concentrations were determined using atomic absorption spectrophotometry and quantitative X-ray microanalysis. Calcium accumulation began in the cuticle at 3 h postmolt at the epicuticle/exocuticle boundary and at the distal and proximal margins of the interprismatic septa (IPS). The bidirectional calcification of the IPS continued until the two fronts met at 5-8 h postmolt. The roughly hexagonal walls of the IPS formed a honeycomb-like structure that resulted in a rigid cuticle. The walls of the canal containing sensory neurons also calcified at 3 h, thereby imparting rigidity to the structure and additional strength to the cuticle. Examination of thin sections of lyophilized cuticle and fixed cuticle revealed that the first mineral deposited is more soluble than calcite and is probably amorphous calcium carbonate. The amorphous calcium carbonate is transformed to calcite along a front that follows the original deposition and is probably controlled by a specialized matrix within the IPS. Since amorphous calcium carbonate is isotropic, it would also make the mineral in the exocuticle stronger by an equal distribution of mechanical stress.

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Lectin Binding by Crustacean Cuticle: The Cuticle of Callinectes sapidus Throughout the Molt Cycle, and the Intermolt Cuticle of Procambarus clarkii and Ocypode quadrata

Cited by 22 publications

References 0 publications

Formation of the arthrodial membrane in the blue crab, Callinectes sapidus

Formation of the arthrodial membrane in the blue crab, Callinectes sapidus

A food web analysis of the juvenile blue crab, Callinectes sapidus , using stable isotopes in whole animals and individual amino acids

Early pattern of calcification in the dorsal carapace of the blue crab,Callinectes sapidus

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