Lecithin nanoparticles enhance the cryosurvival of caprine sperm

Nadri, Touba; Towhidi, Armin; Zeinoaldini, S.; Martínez‐Pastor, Felipe; Mousavi, Mohammad Ali; Noei, R.; Tar, Mohammad; Sangcheshmeh, Abdollah Mohammadi

doi:10.1016/j.theriogenology.2019.04.024

Cited by 28 publications

(37 citation statements)

References 37 publications

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“…É extraída dos grãos de Research, Society and Development, v. 9, n. 8, e751985125, 2020 (CC BY 4.0) | ISSN 2525-3409 | DOI: http://dx.doi.org/10.33448/rsd-v9i8.5125 9 soja, sendo constituida de fosfolipídeos, triglicerídeos e outras substâncias derivadas das refinarias de óleo (Seidman et al, 2002). A lecitina de soja vem sendo acrescida aos meios de criopreservação na forma de nanoparticulas, sendo essas caracterizadas por pequenas micelas que permitem uma melhor interação e revestimento da membrana espermática reduzindo assim os danos causados pelo choque frio e demais eventos causados pelos processos de refrigeração e congelação (Nadri et al, 2019).…”

Section: Crioprotetoresunclassified

“…Dentre os primeiros diluidores disponíveis, compostos por lecitina de soja, estão os Biociphos-Plus ® (IMV®, L'Aigle, France), Andromed ® (Minitub ® , Tiefenbach, Germany) e Bioxcell ® (Biodux, São Paulo) (Nadri et al, 2019).…”

Section: Crioprotetoresunclassified

“…O mesmo foi verificado por Nadri et al (2019) que ao avaliarem o efeito de diferentes diluentes, sendo a base de nano lecitina de soja e um diluente comercial a base de lecitina de soja em diferentes concentrações (1, 2, 3 e 4% de lecitina de soja) e o diluente a base de gema de ovo (15% de gema), sobre a qualidade e a fertilização in vitro do sêmen ovino. Verificaram Research, Society and Development, v. 9, n. 8, e751985125, 2020 (CC BY 4.0) | ISSN 2525-3409 | DOI: http://dx.doi.org/10.33448/rsd-v9i8.5125 10 que o maior percentual de motilidade e menor índice de apoptose das células foram verificados no tratamento à base de nano lecitina de soja a 1% e a 4% quando comparado com os demais tratamentos, sendo verificada maior significância na concentração de 2% de lecitina.…”

Section: Crioprotetoresunclassified

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Efeito da adição de diferentes antioxidantes em diluentes de criopreservação sobre a qualidade do sêmen e a produção in vitro de embriões bovinos

Silva

Marques

Pádua

et al. 2020

RSD

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A qualidade das amostras de sêmen é fundamental para o sucesso dos programas de inseminação artificial em bovinos. Torna-se imprescindível a manutenção das características dos espermatozoides por meio de adequado processo de criopreservação. No entanto, os processos de criopreservação resultam na formação contínua e excessiva de espécies reativas de oxigênio (EROS), que são prejudiciais aos espermatozoides, pois induz a apoptose com consequente redução da qualidade espermática. Dessa forma, têm sido acrescidos aos crioprotetores substâncias antioxidantes como a glutationa e a melatonina, as quais tem como intuito minimizar os efeitos deletérios causados pelas EROS aos espermatozoides, resultando em melhor qualidade espermática, com consequente aumento das taxas de fertilização in vitro. Diante disso, a presente revisão tem por objetivo analisar os efeitos da adição dos antioxidantes glutationa e melatonina em diferentes meios de criopreservação e a produção in vitro de embriões bovinos.

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Section: Crioprotetoresunclassified

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Efeito da adição de diferentes antioxidantes em diluentes de criopreservação sobre a qualidade do sêmen e a produção in vitro de embriões bovinos

Silva

Marques

Pádua

et al. 2020

RSD

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“…With the addition of NRSP in the cryoprotectant, the malondialdehyde (MDA) content decreased and the activity of SOD increased [ 21 ]. Furthermore, lecithin nanoparticles can enhance the cryosurvival of caprine sperm [ 23 ], and albumin coated copper-cysteine nanozyme and Au–Ag-AFT nanozyme both can improve survival of human sperm after cryopreservation by reducing oxidative stress [ 24 , 25 ]. Enrichment of semen extender with selenium nanoparticles improved fertility rate through decreasing peroxidation and injury during cryopreservation of Holstein bulls [ 26 ].…”

Section: Introductionmentioning

confidence: 99%

Single-wall carbon nanotubes improve cell survival rate and reduce oxidative injury in cryopreservation of Agapanthus praecox embryogenic callus

et al. 2020

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Background Cryopreservation is the best way for long-term in vitro preservation of plant germplasm resources. The preliminary studies found that reactive oxygen species (ROS) induced oxidative stress and ice-induced membrane damage are the fundamental causes of cell death in cryopreserved samples. How to improve plant cryopreservation survival rate is an important scientific issue in the cryobiology field. Results This study found that the survival rate was significantly improved by adding single-wall carbon nanotubes (SWCNTs) to plant vitrification solution (PVS) in cryopreservation of Agapanthus praecox embryogenic callus (EC), and analyzed the oxidative response of cells during the control and SWCNTs-added cryopreservation protocol. The SWCNTs entered EC at the step of dehydration and mainly located around the cell wall and in the vesicles, and most of SWCNTs moved out of EC during the dilution step. Combination with physiological index and gene quantitative expression results, SWCNTs affect the ROS signal transduction and antioxidant system response during plant cryopreservation. The EC treated by SWCNTs had higher antioxidant levels, like POD, CAT, and GSH than the control group EC. The EC mainly depended on the AsA-GSH and GPX cycle to scavenge H2O2 in the control cryopreservation, but depended on CAT in the SWCNTs-added cryopreservation which lead to low levels of H2O2 and MDA. The elevated antioxidant level in dehydration by adding SWCNTs enhanced cells resistance to injury during cryopreservation. The ROS signals of EC were balanced and stable in the SWCNTs-added cryopreservation. Conclusions The SWCNTs regulated oxidative stress responses of EC during the process and controlled oxidative damages by the maintenance of ROS homeostasis to achieve a high survival rate after cryopreservation. This study is the first to systematically describe the role of carbon nanomaterial in the regulation of plant oxidative stress response, and provided a novel insight into the application of nanomaterials in the field of cryobiology.

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“…With the addition of NRSP in the cryoprotectant, the malondialdehyde (MDA) content decreased and the activity of SOD increased [21]. Furthermore, lecithin nanoparticles can enhance the cryosurvival of caprine sperm [23], and albumin coated copper-cysteine nanozyme and Au-Ag-AFT nanozyme both can improve survival of human sperm after cryopreservation by reducing oxidative stress [24,25]. Enrichment of semen extender with selenium nanoparticles improved postthaw sperm quality of Holstein bulls, and consequently in vivo fertility rate by reducing lipid peroxidation and sperm damage occurring by cryopreservation [26].…”

Section: Introductionmentioning

confidence: 99%

Single-Wall Carbon Nanotubes Improve Cell Survival Rate and Reduce Oxidative Injury in Cryopreservation of Agapanthus Praecox Embryogenic Callus

Ren

Deng

Chu

et al. 2020

Preprint

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Background: Cryopreservation is the best way for long-term preservation of plant germplasm resources. The preliminary studies found that ROS-induced oxidative stress and ice-induced membrane damage are the fundamental causes of cell death in cryopreserved samples. How to improve plant cryopreservation survival rate is an important scientific issue in the cryobiology field.Results: This study found that the survival rate was significantly improved by adding single-wall carbon nanotubes (SWCNTs) to plant vitrification solution (PVS) in cryopreservation of Agapanthus praecox embryogenic callus (EC), and analyzed the oxidative response of cells during the control and SWCNTs-added cryopreservation protocol. The SWCNTs entered EC at the step of dehydration, and mainly located around the cell wall and in the vesicles, and most of SWCNTs moved out of EC during dilution step. Combined with the physiological and quantitative gene expression results, SWCNTs affect ROS signal transduction and the antioxidant system in plant cryopreservation. The EC treated with SWCNTs exhibited higher antioxidant levels, including POD, CAT and GSH than the control group EC. EC mainly depended on AsA-GSH and GPX cycle to scavenge H2O2 in the control cryopreservation, but depended on CAT in the SWCNTs-added cryopreservation which lead to low levels of H2O2 and MDA. Elevated antioxidant status in dehydration by addition of SWCNTs enhanced cells resistance to cryoinjury. The ROS signals of EC were balanced and stable in the SWCNTs-added cryopreservation.Conclusions: SWCNTs regulated the oxidative stress response of EC during cryopreservation, and controlled cell oxidative damage via the maintenance of ROS homeostasis to achieve high survival rate after cryopreservation. This study is the first time to systematically describe the role of carbon nanomaterials in the regulation of plant oxidative stress response, and provided a novel insight into cell stress response mechanisms in cryopreservation.

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Lecithin nanoparticles enhance the cryosurvival of caprine sperm

Cited by 28 publications

References 37 publications

Efeito da adição de diferentes antioxidantes em diluentes de criopreservação sobre a qualidade do sêmen e a produção in vitro de embriões bovinos

Efeito da adição de diferentes antioxidantes em diluentes de criopreservação sobre a qualidade do sêmen e a produção in vitro de embriões bovinos

Single-wall carbon nanotubes improve cell survival rate and reduce oxidative injury in cryopreservation of Agapanthus praecox embryogenic callus

Single-Wall Carbon Nanotubes Improve Cell Survival Rate and Reduce Oxidative Injury in Cryopreservation of Agapanthus Praecox Embryogenic Callus

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