Lecithin liposomes and microemulsions as new chromatographic phases

Amézqueta, Susana; Fernández-Pumarega, Alejandro; Farré, Sandra; Luna, Daniel; Fuguet, Elisabet; Rosés, Martı́

doi:10.1016/j.chroma.2019.460596

Cited by 14 publications

(7 citation statements)

References 33 publications

(35 reference statements)

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“…For example, the cerasome system was much similar with SPC/Chol system ( d = 0.31) than DSPC/Chol system ( d = 0.40). The reason might be the difference in components between natural and synthetic phospholipids in spite of the morphology of liposomes [30]. Although each method has its own advantages and disadvantages, LEKC is a good choice in terms of simplicity and efficiency.…”

Section: Resultsmentioning

confidence: 99%

“…The SP value is water–solvent partition in a given system, such as log K L/W or log P. The general LFER equation, Eq. (), has been successfully applied to model the solvation that solutes undergo in a biphasic system and includes five different molecular descriptors [30]. To involve ions and ionic species, Abraham [33] introduced an additional term j + J + for cations and an additional term j − J − for anions, and the extension of Eq.…”

Section: Methodsmentioning

confidence: 99%

“…The SP of different model is various. Therefore, a proper method to characterize different partitioning systems is essential to judge the similarity of them [30]. The d distance was considered as an available method to compare different systems, which was calculated from the normalized coefficients of descriptors to study their correlations.…”

Section: Methodsmentioning

confidence: 99%

“…Amézqueta et al. [30] used lecithin as pseudostationary in EKC or mobile phases in LC, respectively. The selectivity of the two EKC systems and a LC system was evaluated through the solvation parameter model; results illustrated that both solute volume and hydrogen bond basicity mainly drove the partition of solute in lecithin chromatography.…”

Section: Introductionmentioning

confidence: 99%

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Determination of lipid–water partition coefficient of neutral and ionic drugs by liposome electrokinetic chromatography

et al. 2021

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Profiling of lipid–water partition coefficients (KL/W) of drugs is an essential issue during the early stage of drug development. In this study, two liposomes, including 1,2‐distearoyl‐sn‐glycero‐3‐phosphocholine (DSPC) + cholesterol (Chol) (DSPC/Chol liposomes) and soybean lecithin (SPC) + Chol (SPC/Chol liposomes), were prepared for the liposome electrokinetic chromatography (LEKC) analysis, and the logarithm of lipid–water partition coefficients (log KL/W) of neutral and ionic drugs were determined based on an iterative method. The log KL/W values determined by the SPC/Chol or DSPC/Chol liposomes LEKC were linearly fitted, which showed a good fitting coefficient (R2 = 0.89). Furthermore, the linear relationship between the data obtained from LEKC system and octanol–water system, immobilized artificial membrane, Caco‐2 cell model, and software prediction was analyzed, respectively. Results illustrated that DSPC/Chol liposomes or SPC/Chol liposomes had a good linear relationship with Caco‐2 cell model, and R2 was 0.81 and 0.72, respectively. Moreover, the linear free energy relationship analysis suggested that the solute volume, hydrogen bond basicity, and J– were the main descriptors that drove the partition process of solutes in the SPC/Chol or DSPC/Chol LEKC system. In addition, the normalized properties of the SPC/Chol and DSPC/Chol LEKC systems through linear free energy relationship analysis were very close. In short, DSPC/Chol liposomes are more suitable for simulating cell membranes than SPC/Chol liposomes, and the developed LEKC is an effective partitioning model for measuring the log KL/W of drugs.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Determination of lipid–water partition coefficient of neutral and ionic drugs by liposome electrokinetic chromatography

et al. 2021

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“…The model of Abraham for neutral solutes has been applied to many liquid chromatography separation systems, including reversed phase , normal phase [38][39][40][41][42] and hydrophobic interaction [43] liquid chromatographies (abbreviated RPLC, NPLC and HILIC, respectively), and even to micellar and microemulsion electrokinetic chromatography systems [31,[44][45][46][47][48][49][50][51][52].…”

Section: Introductionmentioning

confidence: 99%

Linear free energy relationship models for the retention of partially ionized acid-base compounds in reversed-phase liquid chromatography

Soriano-Meseguer

Fuguet

Abraham

et al. 2021

Journal of Chromatography A

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Strategies for capillary electrophoresis: Method development and validation for pharmaceutical and biological applications—Updated and completely revised edition

et al. 2023

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This review is in support of the development of selective, precise, fast, and validated capillary electrophoresis (CE) methods. It follows up a similar article from 1998, Wätzig H, Degenhardt M, Kunkel A. “Strategies for capillary electrophoresis: method development and validation for pharmaceutical and biological applications,” pointing out which fundamentals are still valid and at the same time showing the enormous achievements in the last 25 years. The structures of both reviews are widely similar, in order to facilitate their simultaneous use. Focusing on pharmaceutical and biological applications, the successful use of CE is now demonstrated by more than 600 carefully selected references. Many of those are recent reviews; therefore, a significant overview about the field is provided. There are extra sections about sample pretreatment related to CE and microchip CE, and a completely revised section about method development for protein analytes and biomolecules in general. The general strategies for method development are summed up with regard to selectivity, efficiency, precision, analysis time, limit of detection, sample pretreatment requirements, and validation.

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Lecithin liposomes and microemulsions as new chromatographic phases

Cited by 14 publications

References 33 publications

Determination of lipid–water partition coefficient of neutral and ionic drugs by liposome electrokinetic chromatography

Determination of lipid–water partition coefficient of neutral and ionic drugs by liposome electrokinetic chromatography

Linear free energy relationship models for the retention of partially ionized acid-base compounds in reversed-phase liquid chromatography

Strategies for capillary electrophoresis: Method development and validation for pharmaceutical and biological applications—Updated and completely revised edition

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