Learning about truth and biases through experience: Section surface corrugation, protein denaturation, and staining

Kellenberger, E.

doi:10.1002/(sici)1097-0029(19980701)42:1<33::aid-jemt5>3.0.co;2-s

Cited by 8 publications

(1 citation statement)

References 35 publications

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“…A section whose surface is highly irregular would probably have more epitopes exposed than a smoother section. This appears to be one of the main factors in the use of certain resins for post‐embedding immunocytochemical applications (for a review see Kellenberger, 1998). The usefulness of ultrasmall gold immunoprobes for labelling of plastic sections has been explored (Humbel et al ., 1995).…”

Section: Ultrasmall Gold Immunoprobesmentioning

confidence: 99%

Applications of gold cluster compounds in immunocytochemistry and correlative microscopy: comparison with colloidal gold

Robinson

Tajima

Vandré

2000

Journal of Microscopy

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SummaryIn this review, we discuss the immunocytochemical literature with respect to a comparison between conventional colloidal gold and gold cluster compounds as immunoprobes. The relative advantages and disadvantages of each of these types of particle for immunocytochemical applications are discussed. We present results from our own laboratories and those of others on the comparison of these immunoprobes in selected experimental situations. These results show the use of gold cluster compounds at both light and electron microscope levels. At the ultrastructural level, gold cluster compounds have been used in pre-embedding labelling of cultured cells, and for labelling of ultrathin cryosections and freeze-fracture preparations. Recently, fluorescently tagged gold cluster compounds have become available. Using ultrathin cryosections of human neutrophils as a model system, we demonstrate that a single immunoprobe (i.e. a fluorescently tagged gold cluster compound) is a robust probe for correlative fluorescence and electron microscopy.

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Section: Ultrasmall Gold Immunoprobesmentioning

confidence: 99%