LEAFY homeostasis is regulated via ubiquitin-dependent degradation and sequestration in cytoplasmic condensates

Analysis of 26S Proteasome Activity across Arabidopsis Tissues

“…It is possible that the higher proteasomal activity in tissues such as flowers and roots may be attributed to the temporal developmental stages in plant growth. It has been previously demonstrated that the ubiquitin–proteasome system exhibits high activity in flowers, particularly when there is a pressing need for a rapid downregulation of specific proteins such as transcription factors [ 31 , 32 , 33 , 34 ]. Altogether, L1 and L2 are favorable extraction strategies for precisely investigating the activity and presence of the plant 26S proteasome in distinct tissues.…”

Analysis of 26S Proteasome Activity Across Arabidopsis Tissues

“…It is possible that higher proteasomal activity in tissues such as flowers and roots may be attributed to temporal developmental stages in plant growth. It has been previously demonstrated that the ubiquitin-proteasome system exhibits high activity in flowers, particularly when there is a pressing need for a rapid downregulation of specific proteins such as transcription factors 30-33 . Altogether, L1 and L2 are favorable extraction strategies for precisely investigating the activity and presence of the plant 26S proteasome in distinct tissues.…”

Thinking outside the F‐box: how UFO controls angiosperm development

“…In fact, TF regulation through ubiquitination is a well‐described mechanism (Kodadek et al ., 2006; Geng et al ., 2012) and proteasome‐mediated degradation was shown to be required for UFO‐LFY activity in seedlings (Chae et al ., 2008). However, LFY protein level is globally unaltered in a strong ufo mutant (Chae et al ., 2008) and specific LFY mono‐ or poly‐ubiquitination is insufficiently described in Arabidopsis or petunia (Chae et al ., 2008; Souer et al ., 2008) even if UFO‐mediated degradation of LFY was observed in the cytoplasm in Arabidopsis (Dolde et al ., 2023). The role of a SCF UFO ‐dependent LFY ubiquitination requires further investigations.…”

“…Genetic experiments described above (with ask1 mutant notably; Liu et al ., 2004; Ni et al ., 2004; Wang et al ., 2003) clearly established a role for UFO within a SCF complex. Also, UFO was recently found to regulate LFY level through ubiquitination and degradation in the cytoplasm (Dolde et al ., 2023). This is in opposition with an exclusive role of UFO as a transcriptional cofactor, and the relation between these two functions remains to be determined.…”