Leaf water balance mediated by aquaporins under salt stress and associated glucosinolate synthesis in broccoli

López-Berenguer, Carmen; Martínez-Ballesta, Ma Carmen; García‐Viguera, Cristina; Carvajal, Micaela

doi:10.1016/j.plantsci.2007.11.012

Cited by 107 publications

(72 citation statements)

References 40 publications

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“…Italica, Raphanus sativus L. or the extremophile Thellungiella salsuginea had certain salt tolerance and a high content of glucosinolates under salt stress conditions (López-Berenguer et al 2008;Yuan et al 2010;Pang et al 2012). These results suggested that glucosinolates could play a key role in salt stress response, and they may be involved in the osmotic regulation when water potential decreases due to salinity (Pang et al 2012;López-Berenguer et al 2008). In addition, Pang et al (2012) showed that glucosinolates could act in a different way against salt stress, being the aliphatic glucosinolates the most effective in the osmotic adjustment.…”

Section: Defense Against Abiotic Stressmentioning

confidence: 94%

Biosynthesis and bioactivity of glucosinolates and their production in plant in vitro cultures

Sánchez-Pujante

Borja-Martínez

Pedreño

et al. 2017

Planta

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Main conclusion Glucosinolates are biologically active compounds which are involved in plant defense reaction. The use of plant in vitro cultures and genetic engineering is a promising strategy for their sustainable production.Glucosinolates are a class of secondary metabolites found mainly in Brassicaceae, which contain nitrogen and sulfur in their structures. Glucosinolates are divided into three groups depending on the amino acid from which they are biosynthesized. Aliphatic glucosinolates are generally derived from leucine, valine, methionine, isoleucine and alanine while indole and aromatic glucosinolates are derived from tryptophan and phenylalanine or tyrosine, respectively. These compounds are hydrolyzed by the enzyme myrosinase when plants are stressed by biotic and abiotic factors, obtaining different degradation products. Glucosinolates and their hydrolysis products play an important role in plant defense responses against different types of stresses. In addition, these compounds have beneficial effect on human health because they are strong antioxidants and they have potent cardiovascular, antidiabetic, antimicrobial and antitumoral activities. Due to all the properties described above, the demand for glucosinolates and their hydrolysis products has enormously increased, and therefore, new strategies that allow the production of these compounds to be improved are needed. The use of plant in vitro cultures is emerging as a biotechnological strategy to obtain glucosinolates and their derivatives. This work is focused on the biosynthesis of glucosinolates and the bioactivity of these compounds in plants. In addition, a detailed study on the strategies used to increase the production of several glucosinolates, in particular those synthesized in Brassicaceae, using in vitro plant cultures has been made. Special attention has been paid for increasing the production of glucosinolates and their derivatives using metabolic engineering.

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Section: Defense Against Abiotic Stressmentioning

confidence: 94%

Biosynthesis and bioactivity of glucosinolates and their production in plant in vitro cultures

Sánchez-Pujante

Borja-Martínez

Pedreño

et al. 2017

Planta

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“…In addition, in order to maintain the water balance, cells accumulate secondary metabolites. Glucosinolates, ascorbic acid, phenolic compounds and anthocyanin are the major secondary metabolites involved in plant defense against stress conditions (López-Berenguer et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Influence of salinity on aliphatic and indole glucosinolates in broccoli (Brassica oleracea var. italica)

Sarıkamış¹

2017

Appl Ecol Env Res

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Abstract. Broccoli (Brassica oleracea var. italica) synthesizes sulphur containing plant secondary metabolites known as glucosinolates that are often associated with bioactive properties upon hydrolysis. Glucosinolates are part of plant defence system and the glucosinolate content in plants is usually influenced by several factors including the genetic background of plants, developmental stage, environmental factors and soil conditions. The evaluation of factors affecting glucosinolate content in plants is important to obtain plants with improved bioactive properties. The objective of the present study was to determine the effect of NaCl (0, 40, 80, 100mM) on aliphatic and indole glucosinolate content of broccoli grown in greenhouse conditions. For this purpose, broccoli seedlings at 5-6 leaf were irrigated with 0 (control), 40mM, 80mM and 100mM NaCl. Glucosinolates were determined at three different time points (1, 3, 6 days upon treatment). According to the findings, the amount of individual and total aliphatics and indoles were lower at 40, 80 and 100mM NaCl applications compared to control plants determined at three different time points (P<0.001) suggesting the break down of glucosinolates due to stress conditions. The findings of the study revealed the glucosinolate profile and content of broccoli seedlings under saline conditions.

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“…Moreover, salt stress was also found to influence secondary metabolism in plants. It had also been demonstrated that the content of glucosinolates in radish (Raphanus sativus L.) sprouts (Yuan et al, 2010), canola (Brassica napus L.) (Qasim et al, 2003), and broccoli (Brassica oleracea L.) florets (López-Berenguer et al, 2008) can be changed by the application of NaCl.…”

Section: Introductionmentioning

confidence: 99%

Effect of NaCl treatments on glucosinolate metabolism in broccoli sprouts

Guo

Yuan

Wang

2013

J. Zhejiang Univ. Sci. B

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Abstract:To understand the regulation mechanism of NaCl on glucosinolate metabolism in broccoli sprouts, the germination rate, fresh weight, contents of glucosinolates and sulforaphane, as well as myrosinase activity of broccoli sprouts germinated under 0, 20, 40, 60, 80, and 100 mmol/L of NaCl were investigated in our experiment. The results showed that glucoerucin, glucobrassicin, and 4-hydroxy glucobrassicin in 7-d-old broccoli sprouts were significantly enhanced and the activity of myrosinase was inhibited by 100 mmol/L of NaCl. However, the total glucosinolate content in 7-d-old broccoli sprouts was markedly decreased although the fresh weight was significantly increased after treatment with NaCl at relatively low concentrations (20, 40, and 60 mmol/L). NaCl treatment at the concentration of 60 mmol/L for 5 d maintained higher biomass and comparatively higher content of glucosinolates in sprouts of broccoli with decreased myrosinase activity. A relatively high level of NaCl treatment (100 mmol/L) significantly increased the content of sulforaphane in 7-d-old broccoli sprouts compared with the control. These results indicate that broccoli sprouts grown under a suitable concentration of NaCl could be desirable for human nutrition.

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Leaf water balance mediated by aquaporins under salt stress and associated glucosinolate synthesis in broccoli

Cited by 107 publications

References 40 publications

Biosynthesis and bioactivity of glucosinolates and their production in plant in vitro cultures

Biosynthesis and bioactivity of glucosinolates and their production in plant in vitro cultures

Influence of salinity on aliphatic and indole glucosinolates in broccoli (Brassica oleracea var. italica)

Effect of NaCl treatments on glucosinolate metabolism in broccoli sprouts

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