Leaf-targeted phytochelatin synthase in Arabidopsis thaliana

Peterson, Annita G.; Oliver, David J.

doi:10.1016/j.plaphy.2006.10.021

Cited by 22 publications

(20 citation statements)

References 29 publications

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“…Similarly, increased Cd tolerance was observed in transgenic Brassica juncea plants overexpressing enzymes of GSH biosynthesis, thus exhibiting elevated PC accumulation (Zhu et al 1999a,b). On the contrary, Arabidopsis mutants or transgenic plants with elevated GSH content or PC synthesis did not show enhanced Cd-resistance in comparison with wild type plants (Xiang et al 2001, Lee et al 2003, Peterson and Oliver 2006. Moreover, overexpression of PC synthase in A. thaliana resulted in Cd-hypersensitivity (Lee et al 2003).…”

Section: Discussionmentioning

confidence: 94%

Glutathione in adaptation of Arabidopsis thaliana to cadmium stress

Wójcik¹,

Tukiendorf²

2011

Biol. plant.

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The role of glutathione (GSH) in the adaptation of wild type Arabidopsis thaliana plants to Cd stress was investigated. The nutrient solution (control or containing 50 or 100 μM Cd) was supplemented with buthionine sulfoximine (BSO; 50, 100, 500 μM, to decrease the GSH content in plants) or GSH (50, 100, 500 μM, to increase its content in plants) in order to find how GSH content could regulate Cd stress responses. BSO application did not influence plant biomass, while exogenous GSH (especially 500 μM) reduced root biomass. BSO (500μM) in combination with Cd (100 μM) increased Cd toxicity on root growth (by over 50 %), most probably due to reduced GSH content and phytochelatin (PC) accumulation (by over 96 %). On the other hand, combination of exogenous GSH (500 μM) with Cd (100 μM) was also more toxic to plants than Cd alone despite a significant increase in GSH and PC accumulation (up to 2.7 fold in the roots). This fact could indicate that the natural content of endogenous GSH in wild type A. thaliana plants is sufficient for Cd-tolerance. A decrease in this GSH content led to decreased Cd-tolerance of the plants but an increase in GSH content did not enhance Cd-tolerance, and it showed even toxic effect on the plants.Additional key words: BSO, GSH labelling with monochlorobimane, phytochelatin.

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Section: Discussionmentioning

confidence: 94%

Glutathione in adaptation of Arabidopsis thaliana to cadmium stress

Wójcik¹,

Tukiendorf²

2011

Biol. plant.

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“…PCs are a class of metal‐binding thiol‐peptides playing relevant roles in Cd, Cu, Zn detoxification, and represent a major metal detoxifying system for plants and some other organisms (Sanità di Toppi et al , Bolchi et al , Seth et al ). The Arabidopsis AtPCS1 (= CAD1 ) gene encodes for PCS1, so that cad1 knockout mutants are PC deficient and Cd hypersensitive (Vatamaniuk et al , Peterson and Oliver ). The Arabidopsis genome also contains a highly homologous gene, AtPCS2 , that encodes for a functional PCS apparently non‐redundant with AtPCS1 (Cazalé and Clemens ).…”

Section: Introductionmentioning

confidence: 99%

Correlation between hormonal homeostasis and morphogenic responses in Arabidopsis thaliana seedlings growing in a Cd/Cu/Zn multi‐pollution context

Sofo

Vitti

Nuzzaci

et al. 2013

Physiologia Plantarum

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To date, almost no information is available in roots and shoots of the model plant Arabidopsis thaliana about the hierarchic relationship between metal accumulation, phytohormone levels, and glutathione/phytochelatin content, and how this relation affects root development. For this purpose, specific concentrations of cadmium, copper and zinc, alone or in triple combination, were supplied for 12 days to in vitro growing seedlings. The accumulation of these metals was measured in roots and shoots, and a significant competition in metal uptake was observed. Microscopic analyses revealed that root morphology was affected by metal exposure, and that the levels of trans-zeatin riboside, dihydrozeatin riboside, indole-3-acetic acid and the auxin/cytokinin ratio varied accordingly. By contrast, under metal treatments, minor modifications in gibberellic acid and abscisic acid levels occurred. Real-time polymerase chain reaction analysis of some genes involved in auxin and cytokinin synthesis (e.g. AtNIT in roots and AtIPT in shoots) showed on average a metal up-regulated transcription. The production of thiol-peptides was induced by all the metals, alone or in combination, and the expression of the genes involved in thiol-peptide synthesis (AtGSH1, AtGSH2, AtPCS1 and AtPCS2) was not stimulated by the metals, suggesting a full post-transcriptional control. Results show that the Cd/Cu/Zn-induced changes in root morphology are caused by a hormonal unbalance, mainly governed by the auxin/cytokinin ratio.

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“…Examples include the heterologous expression of A. thaliana AtPCS1 in tobacco [42] and Indian mustard [43], bread wheat TaPCS1 and Ceratophyllum demersum CdPCS1 in tobacco [40], and that of Nelumbo nucifera NnPCS and CdPCS1 in A. thaliana [44]–[46]. When a PCS deficient A. thaliana mutant was transformed with AtPCS1 driven by a leaf-specific promoter, the resulting lines proved less sensitive to Cd toxicity [47]. However, although over-expression of AtPCS1 helped raise the level of tolerance to arsenic [48], it also resulted in a higher sensitivity to Cd [49].…”

Section: Discussionmentioning

confidence: 99%

Cloning and Characterization of a Phragmites australis Phytochelatin Synthase (PaPCS) and Achieving Cd Tolerance in Tall Fescue

et al. 2014

PLoS ONE

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The production of phytochelatins (PCs) provides an important means for plants to achieve tolerance to cadmium (Cd) toxicity. A reed gene encoding PC synthase (PaPCS) was isolated and its function tested through its heterologous expression in a strain of yeast sensitive to Cd. Subsequently, the Cd sensitive and high biomass accumulating species tall fescue was transformed either with PaPCS or PaGCS (a glutamyl cysteine synthetase gene of reed) on their own (single transformants), or with both genes together in the same transgene cassette (double transformant). The single and double transformants showed greater Cd tolerance and accumulated more Cd and PC than wild type plants, and their Cd leaf/root ratio content was higher. The ranking in terms of Cd and PC content for the various transgenic lines was double transformants>PaGCS single transformants>PaPCS single transformants>wild type. Thus PaGCS appears to exert a greater influence than PaPCS over PC synthesis and Cd tolerance/accumulation. The double transformant has interesting potential for phytoremediation.

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Leaf-targeted phytochelatin synthase in Arabidopsis thaliana

Cited by 22 publications

References 29 publications

Glutathione in adaptation of Arabidopsis thaliana to cadmium stress

Glutathione in adaptation of Arabidopsis thaliana to cadmium stress

Correlation between hormonal homeostasis and morphogenic responses in Arabidopsis thaliana seedlings growing in a Cd/Cu/Zn multi‐pollution context

Cloning and Characterization of a Phragmites australis Phytochelatin Synthase (PaPCS) and Achieving Cd Tolerance in Tall Fescue

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