Leaf, Stem, Crown, and Root Galls Induced in Chrysanthemum by Agrobacterium tumefaciens

Miller, H. N.

doi:10.1094/phyto-65-805

Cited by 42 publications

(21 citation statements)

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“…As shown previously (Miller 1975;Miller et al 1975;van Wordragen et al 1991;Bush & Pueppke 1991), differences in the the percentage of tumour formation were observed between chrysanthemum explants inoculated with different wild-type strains of A. tumefaciens. In the present work, the highest frequency of tumour formation was obtained using the strain A6.…”

Section: Host Range Studysupporting

confidence: 72%

A study of some factors affectingAgrobacterium transformation and plant regeneration ofDendranthema grandiflora Tzvelev (syn.Chrysanthemum morifolium Ramat.)

Lowe

Davey

Power

et al. 1993

Plant Cell Tiss Organ Cult

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In an attempt to develop a system for producing transformed plants from explants of Dendranthema grandiflora, the susceptibility of the cultivar Super White to various wild-type strains of Agrobacterium tumefaciens and A. rhizogenes was investigated. Tumour formation was not a reliable indicator of the ability of a related disarmed strain to mediate transformation. Following inoculation of explants with disarmed Agrobacterium strains, a number of shoots developed on selective media. However, none of these shoots were transformed. By co-cultivating stem internode explants with a mixed inoculum of wild-type and disarmed strains, it was possible to obtain a callus stably transformed with Agrobacterium carrying a disarmed T-DNA. Histological analysis of explants revealed that shoot regeneration initially occurred from the cells of the epidermis and subsequently from the cortex. However, the cells which were susceptible to T-DNA transfer were confined to the vascular tissue.

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Section: Host Range Studysupporting

confidence: 72%

A study of some factors affectingAgrobacterium transformation and plant regeneration ofDendranthema grandiflora Tzvelev (syn.Chrysanthemum morifolium Ramat.)

Lowe

Davey

Power

et al. 1993

Plant Cell Tiss Organ Cult

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“…It has been reported that the vir (virulence) regions of the Ti plasmid carried by these strains are similar (Kovács and Pueppke 1994). Both strains have been isolated from Compositae plants: strain Bo542 from dahlia (Montoya et al 1977) and strain Chry5 from chrysanthemum (Miller 1975). The genetic and phylogenetic relationships between strain CNI5 and these supervirulent type strains are interesting topics for future investigation.…”

Section: Resultsmentioning

confidence: 97%

Highly tumorigenic Agrobacterium tumefaciens strain from crown gall tumors of chrysanthemum

Ogawa

Ishikawa

Mii

2000

Archives of Microbiology

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A wild-type Agrobacterium tumefaciens strain CNI5 isolated from crown gall of chrysanthemum (Dendranthema grandiflora Tzvelev) was characterized. Strain CNI5 was classified into biovar 1, based on physiological and biochemical characteristics, and was resistant to ampicillin. Strain CNI5 induced tumors at a higher frequency and on a larger area of explants in most tested plant species, especially in chrysanthemum cultivars, than the octopine-type strain C58C1cmr (pTiB6S3). Agropine and mannopine were detected in tumors induced by strain CNI5 and were specifically catabolized by this strain. Strain CNI5 harbored five plasmids including one plasmid that shared sequence similarity to TL-DNA of the octopine-type Ti plasmid and four cryptic plasmids.

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“…Once the susceptibility of chrysanthemums to Agrobacterium tumefaciens was reported (De Cleene and De Ley 1976;Hooykaas and Beijersbergen 1994;Miller 1975), the Agrobacterium-mediated transformation of chrysanthemum was studied worldwide. However, some barriers to the establishment of a chrysanthemum transformation system have been reported: low transformation e ciency (De Jong et al 1994;Renou et al 1993;Urban et al 1994), regeneration of chimeric plants comprising both transgenic and non-transgenic tissues (Benetka and Pavingerová 1995;Pavingerová et al 1994) and transgene inactivation, also known as gene silencing (Takatsu et al 2000).…”

Section: Agrobacterium-mediated Genetic Transformationmentioning

confidence: 99%

“…Recently, advances in biotechnology have made possible the addition of new traits that are unachievable via conventional or mutation breeding . A er the rst report on the susceptibility of chrysanthemum plants to Agrobacterium (Miller 1975), many researchers have tried to introduce useful agronomical traits into chrysanthemums via Agrobacterium-mediated transformation.…”

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confidence: 99%

Genetic engineering of chrysanthemum (Chrysanthemum morifolium): Current progress and perspectives

Shinoyama¹,

Aida

Ichikawa

et al. 2012

Plant Biotechnology

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We give an overview of the advances of an Agrobacterium-mediated transformation system, clarifying its problems and their solutions, and then show the latest version of our transformation system and examples of the introduction of agronomically important traits into chrysanthemums. Typical problems with the Agrobacterium-mediated transformation in chrysanthemum include low transformation e ciency, high chimerism and cultivar speci city. Using a co-cultivation medium containing acetosyringone and casamino acids for high transformation e ciency and an antibioticselection step for transgenic calli before plant regeneration to eliminate the chimerism, we established an e cient and stable transformation system for chrysanthemum. In addition, this system was used to successfully introduce useful agronomical traits, such as insect resistance and new ower color, into chrysanthemums. ese traits have been stably and highly expressed to confer the expected characteristics upon the transgenic chrysanthemums. Before applying a eld trial of the genetically modi ed (GM) chrysanthemums, male and female sterility were introduced into the transformants to exclude the transgene ow from the GM plants to their wild relatives. So far, using RNAi technology, some of the transgenic chrysanthemums have displayed complete male sterility with very weak female fertility.

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Leaf, Stem, Crown, and Root Galls Induced in Chrysanthemum by Agrobacterium tumefaciens

Cited by 42 publications

References 0 publications

A study of some factors affectingAgrobacterium transformation and plant regeneration ofDendranthema grandiflora Tzvelev (syn.Chrysanthemum morifolium Ramat.)

A study of some factors affectingAgrobacterium transformation and plant regeneration ofDendranthema grandiflora Tzvelev (syn.Chrysanthemum morifolium Ramat.)

Highly tumorigenic Agrobacterium tumefaciens strain from crown gall tumors of chrysanthemum

Genetic engineering of chrysanthemum (Chrysanthemum morifolium): Current progress and perspectives

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