Leaf Regulation of the Nitrogen Concentration in the Grain of Wheat Plants

Barneix, Atilio J.; Guitman, Maria R.

doi:10.1093/jxb/44.10.1607

Cited by 32 publications

(17 citation statements)

References 20 publications

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“…The leaf proteins, mostly Rubisco, are hydrolysed to amino acids during senescence by proteolytic enzymes, and are then exported to the phloem (Ho¨rtensteiner and Feller 2002). It has been shown that GPC is related to the soluble amino acids in the flag leaf (Barneix and Guitman 1993) and to the amount of amino acids exported to the phloem (Caputo et al 2001). The present experiments showed for the first time that at anthesis the flag leaves of the RSL no.…”

Section: Discussionmentioning

confidence: 99%

Nitrogen uptake and remobilization in tetraploid 'Langdon' durum wheat and a recombinant substitution line with the high grain protein gene Gpc-B1

Kade¹,

Barneix²,

Olmos

et al. 2005

Plant Breeding

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Tetraploid wheat (Triticum turgidum L. var. durum) cv. ‘Langdon’ (LDN) and its near‐isogenic recombinant substitution line no. 68 (RSL no. 68) carrying the high grain protein gene Gpc‐B1 from emmer wheat, were compared in three greenhouse experiments to establish in which way Gpc‐B1 increases grain protein concentration (GPC). At anthesis, RSL no. 68 had higher soluble protein and amino acids concentrations in the flag leaf than LDN. At maturity, both lines presented a similar above ground biomass and grain yield. However, RSL no. 68 showed a higher total N content in ears, grain and chaff than LDN; N harvest index (NHI) was also higher because of a lower straw N concentration and higher grain N concentration. When both lines were grown with a low N supply, and when N supply was interrupted before anthesis, similar trends were observed but the differences in GPC were smaller. It is concluded that RSL no. 68 accumulates a higher GPC than LDN mainly because of a more efficient N remobilization from the leaves to the ears during grain filling.

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Section: Discussionmentioning

confidence: 99%

Nitrogen uptake and remobilization in tetraploid 'Langdon' durum wheat and a recombinant substitution line with the high grain protein gene Gpc-B1

Kade¹,

Barneix²,

Olmos

et al. 2005

Plant Breeding

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show abstract

“…The final GPC has been correlated with the free amino acid concentrations in the flag leaf during grain filling (Millet et al, 1992;Barneix and Guitman, 1993), and it has been shown that the ditelosomic line of wheat Chinese Spring CSDT7BL that is deficient in the export of several amino acids to the phloem also shows a decreased GPC compared to the normal line (Caputo et al, 2001). It has also been shown that the high GPC lines of T. turgidum L. var.…”

Section: Source Regulation Of Grain Protein Accumulationmentioning

confidence: 99%

Physiology and biochemistry of source-regulated protein accumulation in the wheat grain

Barneix

2007

Journal of Plant Physiology

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174

119

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“…In fact, contrary to the main substrate source for grain starch, the vital source of grain protein is the remobilization of pre-anthesis stored nitrogen in vegetative organs in vivo, such as leaf (Dalling et al 1976; Smith and Whitfield 1990). Because grain protein synthesis is substrate-limited by the amino acid export from the leaf protein pool in vivo (Barneix and Guitman, 1993), it is helpful to understand the actual effects of exogenous hormones on N mobilization and grain protein accumulation using our ear culture system with flag leaves and no N nutrient added to the culture media. From anthesis, the net photosynthetic rate of the flag leaf declines gradually and then sharply with leaf aging process in wheat.…”

Section: Treatmentmentioning

confidence: 99%

“…The negative effects of ZR on N translocation are similar to the previous study by Sun et al (1996). As the remobilized leaf protein during post-anthesis is usually a vital source of grain storage protein (Dalling et al 1976;Smith and Whitfield 1990;Barneix and Guitman 1993), ABA may benefit grain protein accumulation, whereas ZR may not.…”

Section: Treatmentmentioning

confidence: 99%

Effects of exogenous ABA and cytokinin on leaf photosynthesis and grain protein accumulation in wheat ears cultured in vitro

Xie

Jiang

Dai

et al. 2004

Plant Growth Regulation

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A detached culture system and steady-state 15 N labeling technique were used to study the effects of exogenous ABA and ZR on photosynthetic characteristics, nitrogen remobilization and the activities of key enzymes for nitrogen metabolism in detached wheat parts during grain protein accumulation. The differences in net photosynthetic rate, chlorophyll content (SPAD value) and soluble protein content in the flag leaves of detached culture system between the treatments of ABA and ZR showed that ABA facilitates the post-anthesis senescence course compared to the ZR treatment. The differences in the changes of 15 N amount in different organs in the detached culture system between the ABA and ZR treatments showed that nitrogen remobilization from vegetative organs to the grain is accelerated by the ABA treatment but is delayed by ZR. The activities of GS and GPT in grains treated with ABA were significantly higher than those with the control treatment at 5 DAC, but reduced significantly compared with control at 11 DAC. The two enzyme activities in grains were reduced significantly by ZR at 5 DAC and increased significantly at 11 DAC, compared with those treated with ABA. The above changes of enzyme activity showed that the ABA treatment hastens amino acid conversion into grains and protein accumulation in grains, whereas the ZR treatment delays these processes. A significant reduction in grain weight with ABA treatment is associated with the reduction of net photosynthesis, chlorophyll content, and soluble protein content in flag leaves. Compared with the control and ZR treatments, a significant increase in grain protein content with the ABA treatment may result from the accelerating effects of ABA on N remobilization, amino acid conversion into grains and protein accumulation in grains.Abbreviations: ABA -abscisic acid; DAC -days after culture; GPT -glutamate pyruvate transaminase; GS -glutamine synthetase; ZR -zeatin riboside; RuBPCO -ribulose bisphosphate carboxylase oxygenase

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Leaf Regulation of the Nitrogen Concentration in the Grain of Wheat Plants

Cited by 32 publications

References 20 publications

Nitrogen uptake and remobilization in tetraploid 'Langdon' durum wheat and a recombinant substitution line with the high grain protein gene Gpc-B1

Nitrogen uptake and remobilization in tetraploid 'Langdon' durum wheat and a recombinant substitution line with the high grain protein gene Gpc-B1

Physiology and biochemistry of source-regulated protein accumulation in the wheat grain

Effects of exogenous ABA and cytokinin on leaf photosynthesis and grain protein accumulation in wheat ears cultured in vitro

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