Leaf proteome characterization in the context of physiological and morphological changes in response to copper stress in sorghum

Roy, Swapan Kumar; Kwon, Soo Jeong; Cho, Seongwoo; Kamal, Abu Hena Mostafa; Kim, Sang Woo; Sarker, Kabita; Oh, Myeong-Won; Lee, Moon-Soon; Chung, Keun-Yook; Xin, Zhanguo; Woo, Sun–Hee

doi:10.1007/s10534-016-9932-6

Cited by 22 publications

(15 citation statements)

References 88 publications

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“…They concluded that protein secretion is a major response of sorghum to osmotic stress [34]. Another study of sorghum also used physiological and comparative proteomics to study abiotic stress response and to identify protein groups responding to drought, salinity, and Cd/Cu stress in sorghum [35][36][37][38][39][40][41].…”

Section: Introductionmentioning

confidence: 99%

Physiological and Differential Proteomic Analyses of Imitation Drought Stress Response in Sorghum bicolor Root at the Seedling Stage

Li²,

Ke³

et al. 2020

IJMS

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Drought is one of the most important constraints on the growth and productivity of many crops, including sorghum. However, as a primary sensing organ, the plant root response to drought has not been well documented at the proteomic level. In the present study, we compared physiological alteration and differential accumulation of proteins in the roots of sorghum (Sorghum bicolor) inbred line BT×623 response to Polyethylene Glycol (PEG)-induced drought stress at the seedling stage. Drought stress (up to 24 h after PEG treatment) resulted in increased accumulation of reactive oxygen species (ROS) and subsequent lipid peroxidation. The proline content was increased in drought-stressed plants. The physiological mechanism of sorghum root response to drought was attributed to the elimination of harmful free radicals and to the alleviation of oxidative stress via the synergistic action of antioxidant enzymes, such as superoxide dismutase, peroxidase, and polyphenol oxidase. The high-resolution proteome map demonstrated significant variations in about 65 protein spots detected on Coomassie Brilliant Blue-stained 2-DE gels. Of these, 52 protein spots were identified by matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry (MALDI-TOF-TOF MS) representing 49 unique proteins; the levels of 43 protein spots were increased, and 22 were decreased under drought condition. The proteins identified in this study are involved in a variety of cellular functions, including carbohydrate and energy metabolism, antioxidant and defense response, protein synthesis/processing/degradation, transcriptional regulation, amino acid biosynthesis, and nitrogen metabolism, which contribute jointly to the molecular mechanism of outstanding drought tolerance in sorghum plants. Analysis of protein expression patterns and physiological analysis revealed that proteins associated with changes in energy usage; osmotic adjustment; ROS scavenging; and protein synthesis, processing, and proteolysis play important roles in maintaining root growth under drought stress. This study provides new insight for better understanding of the molecular basis of drought stress responses, aiming to improve plant drought tolerance for enhanced yield.

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Section: Introductionmentioning

confidence: 99%

Physiological and Differential Proteomic Analyses of Imitation Drought Stress Response in Sorghum bicolor Root at the Seedling Stage

Li²,

Ke³

et al. 2020

IJMS

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“…Most reports, however, have focused on herbaceous plants, including rice [17][18][19], Allium cepa [20], Oenothera glazioviana [21], Arabidopsis [22], Cannabis sativa [23], Agrostis capillaris [24], Elsholtzia splendens [25,26], sorghum [27,28] and wheat [29], while only one study investigated Cu-toxic effects on protein profiles in leaves of woody plant Eucalyptus camaldulensis [30]. Also, most of the above studies mainly focused on Cu-toxicity-responsive proteins occurring in roots because Cu is preferentially accumulated in Cu-stressed roots, while only few studies investigated Cu-toxic effects on protein profiles in leaves [25,27,29,30]. Evidence shows that the toxic effects of Cu on plant proteomics vary with Cu concentration, plant species, populations and/or cultivars, and plant tissues [17,18,24,25,[27][28][29].…”

Section: Introductionmentioning

confidence: 99%

“…Also, most of the above studies mainly focused on Cu-toxicity-responsive proteins occurring in roots because Cu is preferentially accumulated in Cu-stressed roots, while only few studies investigated Cu-toxic effects on protein profiles in leaves [25,27,29,30]. Evidence shows that the toxic effects of Cu on plant proteomics vary with Cu concentration, plant species, populations and/or cultivars, and plant tissues [17,18,24,25,[27][28][29]. Therefore, more extensive proteomic research on the leaves of woody plants is needed to elucidate the molecular mechanisms of plants under Cu-toxicity.…”

Section: Introductionmentioning

confidence: 99%

Excess Copper-Induced Alterations of Protein Profiles and Related Physiological Parameters in Citrus Leaves

Huang

et al. 2020

Plants

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This present study examined excess copper (Cu) effects on seedling growth, leaf Cu concentration, gas exchange, and protein profiles identified by a two-dimensional electrophoresis (2-DE) based mass spectrometry (MS) approach after Citrus sinensis and Citrus grandis seedlings were treated for six months with 0.5 (control), 200, 300, or 400 μM CuCl2. Forty-one and 37 differentially abundant protein (DAP) spots were identified in Cu-treated C. grandis and C. sinensis leaves, respectively, including some novel DAPs that were not reported in leaves and/or roots. Most of these DAPs were identified only in C. grandis or C. sinensis leaves. More DAPs increased in abundances than DAPs decreased in abundances were observed in Cu-treated C. grandis leaves, but the opposite was true in Cu-treated C. sinensis leaves. Over 50% of DAPs were associated with photosynthesis, carbohydrate, and energy metabolism. Cu-toxicity-induced reduction in leaf CO2 assimilation might be caused by decreased abundances of proteins related to photosynthetic electron transport chain (PETC) and CO2 assimilation. Cu-effects on PETC were more pronounced in C. sinensis leaves than in C. grandis leaves. DAPs related to antioxidation and detoxification, protein folding and assembly (viz., chaperones and folding catalysts), and signal transduction might be involved in Citrus Cu-toxicity and Cu-tolerance.

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“…Protein extraction from the 10-LS and FS leaves of the sesame seedling was performed using the trichloroacetoc acid / acetone precipitation method as described Roy et al (2016). About 500 mg of the frozen leaf tissue was ground into a fine powder in liquid nitrogen.…”

Section: Determination Of Plant Height Stem Diameter and Leaf Chlormentioning

confidence: 99%

Proteome analysis of sesame leaves in response to waterlogging stress at vegetative and flowering stages

Jung¹,

Roy²,

Cho³

et al. 2019

Biologia plant.

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Waterlogging, a major environmental stress, impairs plant growth and development and induces synthesis of different proteins. To understand the molecular mechanisms coupled with morpho-physiological alterations underlying waterlogging tolerance, the LTQ-FTICR MS/MS technique was employed to map the proteomes of leaves of sesame grown under control and waterlogged conditions. The waterlogging treatment caused dramatic alterations in morphological and biochemical properties of the leaves of sesame. For proteome analysis, more than 75 reproducible protein spots were identified on 2-DE gels wherein 51 protein spots (≥ 1.5-fold change) were used for analysis by mass spectrometry. Among 51 differentially abundant proteins, 20 were specific to the 10-leaf stage and 31 were specific to the flowering stage. Most of the differentially abundant proteins were involved in group metabolism, and energy and stress defense. Oxygen-evolving enhancer protein 1, ATP synthase subunit, heat shock proteins, glutamine synthetase, glyceraldehyde-3-phosphate dehydrogenase, and superoxide dismutase were upregulated under waterlogging. However, the photosynthesis-and protein biosynthesisrelated proteins (e.g., ribulose-1,5-bisphosphate carboxylase/oxygenase activase, and S-adenosylmethionine synthase 1) were down-regulated under waterlogging. The protein interaction network indicates that energy metabolism-and stressand defense-related proteins were involved in the protein-protein interaction network, which could form an indispensable network in sesame leaves. To this end, physiological results highlighted the impairment of photosyntheis, which is consistent with results obtained at the proteome level. The upregulation of metabolism-, energy-, and stress defenserelated proteins in response to waterlogging stress may provide new insights into the complex mechanisms underlying waterlogging tolerance in sesame.

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Leaf proteome characterization in the context of physiological and morphological changes in response to copper stress in sorghum

Cited by 22 publications

References 88 publications

Physiological and Differential Proteomic Analyses of Imitation Drought Stress Response in Sorghum bicolor Root at the Seedling Stage

Physiological and Differential Proteomic Analyses of Imitation Drought Stress Response in Sorghum bicolor Root at the Seedling Stage

Excess Copper-Induced Alterations of Protein Profiles and Related Physiological Parameters in Citrus Leaves

Proteome analysis of sesame leaves in response to waterlogging stress at vegetative and flowering stages

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