Leaf morphological and genetic differentiation between Quercus robur L. and its closest relative, the drought-tolerant Quercus pedunculiflora K. Koch

Curtu, Alexandru Lucian; Șofletea, N.; Toader, A. V.; Enescu, C. M.

doi:10.1007/s13595-011-0105-z

Cited by 23 publications

(12 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As result of interspecifi c gene fl ow and incomplete lineage sorting, genetic differentiation at nuclear (Mariette et al 2002, Muir & Schlötterer 2005, Craft & Ashley 2006, Curtu et al 2011b, Vidalis et al 2013) and cpDNA markers (Whittemore & Schaal 1991, Petit et al 2002 is very low between closely related hybridizing oak species. For example, analysis of eight genomic microsatellites in seven mixed stands of Q. robur and Q. petraea revealed that only 1.5 % of the genetic differentiation was distributed between species (Mariette et al 2002).…”

Section: Evidence For Divergent Selectionmentioning

confidence: 99%

Interspecific gene flow and maintenance of species integrity in oaks

Gailing

Curtu

2014

Ann. For. Res.

Self Cite

View full text Add to dashboard Cite

Abstract. Oak species show a wide variation in morphological and physiological characters, and boundaries between closely related species are often not clear-cut. Still, despite frequent interspecific gene flow, oaks maintain distinct morphological and physiological adaptations. In sympatric stands, spatial distribution of species with different ecological requirements is not random but constrained by soil and other micro-environmental factors. Here, we discuss factors that may influence the maintenance of the integrity of oak species in the face of interspecific gene flow. Pre-zygotic isolation (e.g. cross incompatibilities, asynchrony in flowering, pollen competition) and post-zygotic isolation (divergent selection) contribute to the maintenance of species integrity in sympatric oak stands. The antagonistic effects of interspecific gene flow and divergent selection are reflected in the low genetic differentiation between hybridizing oak species at most genomic regions interspersed by regions with signatures of divergent selection (outlier regions). In the near future, the availability of high-density genetic linkage maps anchored to scaffolds of a sequenced Q. robur genome will allow to characterize the underlying genes in these outlier regions and their putative role in reproductive isolation between species. Reciprocal transplant experiments of seedlings between parental environments can be used to characterize selection on outlier genes. High transferability of gene-based markers will enable comparative outlier screens in different oak species.

show abstract

Section: Evidence For Divergent Selectionmentioning

confidence: 99%

Interspecific gene flow and maintenance of species integrity in oaks

Gailing

Curtu

2014

Ann. For. Res.

Self Cite

View full text Add to dashboard Cite

show abstract

“…North American oaks also synthesize terpenoid molecules, such as isoprene, while some clades of European oaks produce other terpenoids, for example, monoterpenes [ 21 , 22 , 23 ]. Therefore typically soft, non- photosynthesizing tissues (such as young roots, germinating seedlings, buds, flowers or fruits) or young, developing leaves are preferred for genomic DNA isolation from these species as they contain higher amounts of DNA and lower amounts of potential inhibitory metabolites than mature leaves [ 24 , 25 , 26 , 27 ]. However, these tissues are often available only briefly during the lifecycle of many long-lived woody species, unless they are maintained under controlled conditions or in vitro, in tissue, or protoplast cultures, which require time consuming and costly preparation, maintenance, and additional resources that often unavailable at education centered, applied learning institutions [ 28 , 29 ].…”

Section: Introductionmentioning

confidence: 99%

“…The season-long availability of mature leaves is often greater for many woody species of interest for genomic studies in temperate regions, or year-round in some species, such as live oaks ( Quercus virginiana ) in the Southern United States or the Japanese evergreen oaks ( Quercus acuta ) in Eastern Asia. However, their direct applications are limited due to the difficulties in isolating high quality and quantity DNA from the metabolite rich tissue [ 24 , 25 , 26 , 27 ].…”

Section: Introductionmentioning

confidence: 99%

“…Commercial kits are relatively ineffective in extracting DNA from mature leaves of many species of interest [ 10 , 11 , 12 , 13 , 14 , 24 , 25 , 26 , 27 , 30 , 31 ] and require time-consuming and costly pre- and/or post-processing of samples. This may include the digestion of cell walls, isolation of intact protoplasts, enzymatic break down of sugars, isolation of nuclei, mitochondria or chloroplasts, the concentration of the extract, or the separation of DNA from contaminants [ 3 , 5 , 32 , 33 , 34 , 35 ].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

In Situ Dark Adaptation Enhances the Efficiency of DNA Extraction from Mature Pin Oak (Quercus palustris) Leaves, Facilitating the Identification of Partial Sequences of the 18S rRNA and Isoprene Synthase (IspS) Genes

Barta

Bolander

Bilby

et al. 2017

Plants

View full text Add to dashboard Cite

Mature oak (Quercus spp.) leaves, although abundantly available during the plants’ developmental cycle, are rarely exploited as viable sources of genomic DNA. These leaves are rich in metabolites difficult to remove during standard DNA purification, interfering with downstream molecular genetics applications. The current work assessed whether in situ dark adaptation, to deplete sugar reserves and inhibit secondary metabolite synthesis could compensate for the difficulties encountered when isolating DNA from mature leaves rich in secondary metabolites. We optimized a rapid, commercial kit based method to extract genomic DNA from dark- and light-adapted leaves. We demonstrated that in situ dark adaptation increases the yield and quality of genomic DNA obtained from mature oak leaves, yielding templates of sufficiently high quality for direct downstream applications, such as PCR amplification and gene identification. The quality of templates isolated from dark-adapted pin oak leaves particularly improved the amplification of larger fragments in our experiments. From DNA extracts prepared with our optimized method, we identified for the first time partial segments of the genes encoding 18S rRNA and isoprene synthase (IspS) from pin oak (Quercus palustris), whose full genome has not yet been sequenced.

show abstract

“…Leaf descriptors are the most commonly used morphological variables for distinguishing between oak species. Leaf morphology of European white oak species has been studied extensively using both traditional morphometric methods (Kremer et al 2002;Gugerli et al 2007;Curtu et al 2011b) and geometric morphometric methods ). In the last 2 decades, different types of genetic markers have been used for species delimitations (Petit and Excoffier 2009).…”

Section: Introductionmentioning

confidence: 99%

Is Quercus virgiliana a distinct morphological and genetic entity among European white oaks?

Enescu¹,

Curtu²,

Șofletea³

2013

Turk J Agric For

Self Cite

View full text Add to dashboard Cite

2-Morpholine-4ylethyl-3 H -1,2,4-triazole-3-ones (2a, 2b) were obtained from the condensation between the corresponding ethoxycarbonylhydrazones and 2-morpholinoethanamine. 2a was converted to acetohydrazide (4) via the formation of an ester derivative (3). Treatment of 2a and 2b with several aryl sulfonyl chlorides afforded the corresponding 2-arylsulfonyl-1,2,4-triazole-3-ones (5a-c and 6). The reaction of hydrazide (4) with benzyl iso-and benzyl isothiocyanate produced the corresponding carboxamide (8a) and carbothioamide (8b). The basic treatment of 8b yielded 5-mercapto-4 H -1,2,4-triazol-3yl)methyl]-2,4-dihydro-3 H -1,2,4-triazol-3-one (10). The synthesis of 1,3-thiazol-2(3 H) -ylidene-1,2,4-triazol-1-ylacetohydrazide (11) and 1,3-oxazole-2(3 H) -ylidene-1,2,4-triazole-1-yl)acetohydrazide ( 9) derivatives was performed from the reaction of 8a and 8b with substituted phenacyl bromides.All the newly synthesized compounds were screened for their antimicrobial activities and some of them were found to possess good or moderate antimicrobial activity.

show abstract

Leaf morphological and genetic differentiation between Quercus robur L. and its closest relative, the drought-tolerant Quercus pedunculiflora K. Koch

Abstract: To cite this version:Alexandru Curtu, Sofletea, Alin Toader, Mihai Enescu. Leaf morphological and genetic differentiation between Quercus robur L. and its closest relative, the drought-tolerant Quercus pedunculiflora

Cited by 23 publications

References 31 publications

Interspecific gene flow and maintenance of species integrity in oaks

Interspecific gene flow and maintenance of species integrity in oaks

In Situ Dark Adaptation Enhances the Efficiency of DNA Extraction from Mature Pin Oak (Quercus palustris) Leaves, Facilitating the Identification of Partial Sequences of the 18S rRNA and Isoprene Synthase (IspS) Genes

Is Quercus virgiliana a distinct morphological and genetic entity among European white oaks?

Contact Info

Product

Resources

About