Leaf metabolomic data of eight sunflower lines and their sixteen hybrids under water deficit

Berton, Thierry; Bernillon, Stéphane; Fernández, Olivier; Duruflé, Harold; Flandin, Amélie; Cassan, Cédric; Jacob, Daniel; Langlade, Nicolas B.; Gibon, Yves; Moing, Annick

doi:10.1051/ocl/2021029

Cited by 3 publications

(5 citation statements)

References 16 publications

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“…Most of the tentatively annotated metabolites belonged to four biochemical classes, namely terpenes, flavonoids, polyacetylenes and cinnamic acids (Table 1 ). These findings were in line with our previous research on sunflower, and specifically with the works focusing on drought stress [ 16 , 18 ]. However, the detection of polyacetylenes was unique to this study.…”

Section: Discussionsupporting

confidence: 93%

“…After the partial removal of redundancy due to isotopes and adducts, the final metabolome dataset consisted of 2557 LC-MS features (Table S 01 ) characterized by a retention time (RT) and a mass over charge ratio ( m/z) (Table S 02 ). A total of 21 features already had an annotation based on previous works (Table S 02 ) [ 16 , 18 , 27 ].…”

Section: Resultsmentioning

confidence: 99%

“…A total of 950 redundant variables were removed, thus bringing the final LC-MS dataset to 2557 features. The most intense ions were then annotated using RT and accurate m/z values and the information available from previous studies [ 16 , 18 , 27 ]. This resulted in the putative annotation of 21 compounds (Table S 02 ), whose MSI levels were attributed according to [ 80 ].…”

Section: Methodsmentioning

confidence: 99%

“…The molecular mechanisms underlying tolerance to abiotic stresses in sunflower have been studied using different approaches over the last years. Non-targeted metabolomics and proteomics have been used to profile a set of inbred lines and hybrid genotypes [ 16 , 17 ] and to find biomarkers for drought tolerance [ 18 ], while transcriptome and metabolome have been integrated to identify transcription factors regulated under the same condition [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

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Genetic control of abiotic stress-related specialized metabolites in sunflower

Moroldo,

Blanchet,

Duruflé

et al. 2024

BMC Genomics

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Background Abiotic stresses in plants include all the environmental conditions that significantly reduce yields, like drought and heat. One of the most significant effects they exert at the cellular level is the accumulation of reactive oxygen species, which cause extensive damage. Plants possess two mechanisms to counter these molecules, i.e. detoxifying enzymes and non-enzymatic antioxidants, which include many classes of specialized metabolites. Sunflower, the fourth global oilseed, is considered moderately drought resistant. Abiotic stress tolerance in this crop has been studied using many approaches, but the control of specialized metabolites in this context remains poorly understood. Here, we performed the first genome-wide association study using abiotic stress-related specialized metabolites as molecular phenotypes in sunflower. After analyzing leaf specialized metabolites of 450 hybrids using liquid chromatography-mass spectrometry, we selected a subset of these compounds based on their association with previously known abiotic stress-related quantitative trait loci. Eventually, we characterized these molecules and their associated genes. Results We putatively annotated 30 compounds which co-localized with abiotic stress-related quantitative trait loci and which were associated to seven most likely candidate genes. A large proportion of these compounds were potential antioxidants, which was in agreement with the role of specialized metabolites in abiotic stresses. The seven associated most likely candidate genes, instead, mainly belonged to cytochromes P450 and glycosyltransferases, two large superfamilies which catalyze greatly diverse reactions and create a wide variety of chemical modifications. This was consistent with the high plasticity of specialized metabolism in plants. Conclusions This is the first characterization of the genetic control of abiotic stress-related specialized metabolites in sunflower. By providing hints concerning the importance of antioxidant molecules in this biological context, and by highlighting some of the potential molecular mechanisms underlying their biosynthesis, it could pave the way for novel applications in breeding. Although further analyses will be required to better understand this topic, studying how antioxidants contribute to the tolerance to abiotic stresses in sunflower appears as a promising area of research.

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Section: Discussionsupporting

confidence: 93%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Genetic control of abiotic stress-related specialized metabolites in sunflower

Moroldo,

Blanchet,

Duruflé

et al. 2024

BMC Genomics

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“…Researchers are apparently increasingly focusing on the impact of global climate change, especially high temperatures and drought. Authors (Berton at al., 2021) provided untargeted and targeted metabolomic data of sunflower leaves under water deficit. They constitute a valuable resource for the community to study the adaptation of crops to drought and the metabolic bases of heterosis.…”

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confidence: 99%

Introduction to the Special Issue Sunflower

Miklič¹

2022

OCL

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Can Modification of Sowing Date and Genotype Selection Reduce the Impact of Climate Change on Sunflower Seed Production?

Krstić,

Mladenov,

Banjac

et al. 2023

Agriculture

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Climate change projections for the 21st century pose great threats to semi-arid regions, impacting seed production and the quality of sunflowers. Crop yields are negatively affected by climate variability, especially in the event of droughts during the crucial growth stages. Understanding the relationships between agrometeorological, genetic, and agronomic factors is crucial for maintaining crop sustainability. Optimal sowing dates are an essential condition for maximizing crop genetic potential, but challenges come from annual weather variations. This study analyzes how sunflower genotypes respond to different sowing dates under climate change and focuses on the conditions for obtaining maximum seed yields and favorable agronomic traits. From 2020 to 2022, the experiment featured six genotypes sown across four different dates at two-week intervals, simulating seed sunflower production. The results obtained by ANOVA indicated that the seed yield and oil yield were significantly affected by the sowing date, the genotype, and their interaction, with coefficients of variation ranging from 7.6% for oil yield to 41.1% for seed yield. Besides seed yield and oil yield, LDA biplot and Discriminant Functions confirmed that seed germination energy also played a significant role in separating genotypes into clusters. A Visual Mixed Model showed that shifting the optimal sowing date (mid-April) to early May allows a reduction in the number of days the plants spend in critical growth stages, thereby escaping stressful conditions during pollination and seed filling. The findings resulted, on average, in increased yields and improved seed quality, which are the primary goals of seed production, but not in increased 1000-seed weight. Notably, high temperatures during the critical sunflower growth stages negatively affected the measured parameters of seed production. The increased precipitation during seed filling boosted the 1000-seed mass and seed yield. Extended flowering reduced the growth rate and seed germination, but longer seed filling increased the 1000-seed mass and seed yield. Our future breeding goals will be to create genotypes with a shorter flowering period and an extended seed-filling period to better respond to climate change.

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Leaf metabolomic data of eight sunflower lines and their sixteen hybrids under water deficit

Cited by 3 publications

References 16 publications

Genetic control of abiotic stress-related specialized metabolites in sunflower

Genetic control of abiotic stress-related specialized metabolites in sunflower

Introduction to the Special Issue Sunflower

Can Modification of Sowing Date and Genotype Selection Reduce the Impact of Climate Change on Sunflower Seed Production?

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