Leaf geometric morphometrics among populations of Dalbergia ecastaphyllum (L.) Taub.

Morais, Daniel Vieira de; Nunes, Lorena Andrade; Mata, Vandira Pereira da; Costa, Mirco; Sodré, Geni da Silva; Carvalho, Carlos Alfredo Lopes de

doi:10.14393/bj-v35n6a2019-39814

Cited by 6 publications

(5 citation statements)

References 16 publications

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“…Several species classification studies describe a percentage value of discriminant precision greater than 90% to verify that the DFA is correct [73][74][75]. However, the sample size of this study allows flexibility in the percentage of precision for the validity of the classification [76].…”

Section: Flowersmentioning

confidence: 99%

Geometry morphometrics of plant structures as a phenotypic tool to differentiate Polylepis incana Kunth. and Polylepis racemosa Ruiz & Pav. reforested jointly in Ecuador

Guamba

Corredor

Galárraga

et al. 2021

Neotropical Biodiversity

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In Ecuador, there is a progressive loss of the native forest. To mitigate these problems, several reforestation plans have been carried out in the country. To recover the Andean forest, Polylepis racemosa was introduced from Peru, due to its similarity to native species like Polylepis incana. This genus fulfills important ecological functions that help mitigate the effects of local climate change. However, reforestation of introduced species close to natural populations threatens the genetic diversity of Polylepis native forests. In the long term, it can trigger hybridization processes and create problems for ecosystem conservation. This study used geometry morphometric (GM), to differentiate species quickly and economically, using the form of leaves, stomata, flowers, and pollen of P. incana and P. racemosa in three populations: Illinizas Ecological Reserve, Mojanda Lagoons and Cayambe-Coca National Park. To obtain the data, the composite leaves were scanned, the stomata and pollen were photographed with an increase of 400× and on flowers with 100×. 15, 10, 12, and 9 landmarks (LM) were digitized for leaves, stomata, flowers, and pollen, respectively, using ImageJ software. Then, the shape variables and statistical analysis were performed in MorphoJ software. As a result, it was obtained that the discriminant function analysis (DFA) shows that leaves and flowers can be used as indicators to differentiate species from this genus, discarding stomata and pollen. After that, Canonical Variate Analysis (CVA) of leaves and flowers showed that reforested species jointly are separated into two different species; except for the flowers of the population of Mojanda, where there is an intersection of few individuals, which share similar phenotypic characteristics so they can be considered as potentially hybrid individuals. This study generates information on the location of species introduced inside and outside the National System of Protected Areas that threaten the Andean highlands. Finally, GM is an accessible tool for monitoring biodiversity through morphological characteristics and discriminating against species with complex taxonomic problems.

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Section: Flowersmentioning

confidence: 99%

Geometry morphometrics of plant structures as a phenotypic tool to differentiate Polylepis incana Kunth. and Polylepis racemosa Ruiz & Pav. reforested jointly in Ecuador

Guamba

Corredor

Galárraga

et al. 2021

Neotropical Biodiversity

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“…The taxonomy of Geunsia is extremely complex due to wide geographical distribution, species overlap, high levels of variability, and phenotypic plasticity (Kawakubo 1986;Palmer 2012). These variables were often observed through their effects on the leaf structures (Vieira et al 2014;Li et al 2018;Morais et al 2019). Therefore, leaf morphology is considered the most valuable answer in identifying taxa particularly for species belonging to the family Lamiaceae (Balant et al 2019).…”

Section: Introductionmentioning

confidence: 99%

Leaf geometric morphometric analyses of Callicarpa and Geunsia (Lamiaceae) in the Malesian region

Danila

Alejandro

2021

Biodiversitas

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Abstract. Danila JS, Alejandro GJD. 2021. Leaf geometric morphometric analyses of Callicarpa and Geunsia (Lamiaceae) in the Malesian region. Biodiversitas 22: 4379-4390. Leaves are one of the most substantial organs of plants for it serves as a basis of species identification. Leaf morphology provides distinguishing features that help in the discrimination of plant species as well as investigation of leaf features among populations. This study aimed to investigate leaf shape variations between the two genera Geunsia Blume group and its closely related taxon, Callicarpa L. (Lamiaceae) using a landmark-based geometric morphometric method. The differences in the leaf shape among former members of Geunsia, namely C. apoensis, C. basilanensis, C. flavida, C. paloensis, C. pentandra, C. ramiflora, and C. surigaensis are also evaluated. Two primary landmarks and 14 semilandmarks were assigned in all samples to represent changes around the leaf margin. The Procrustes fit was generated using MorphoJ software which displays the mean and landmark position for individual configurations. Canonical Variate Analysis (CVA) and Mahalanobis Distance (MD) were able to discriminate all samples of Geunsia species using a scatter plot. Furthermore, Procrustes ANOVA showed a significant difference (P = 0.0082) among the seven species of the Geunsia group. Based on the results obtained, geometric morphometrics of leaf shape is effective in interspecific discrimination within members of Geunsia. However, the result of Discriminant Analysis (DA) showed that Geunsia and Callicarpa groups made leaf shape differences inefficient in discriminating the two genera. Therefore, further morphological studies on landmark-based geometric morphometrics of leaf shape involving a larger number of samples especially in the study of intergeneric classification are suggested.

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“…In the last decade, in-depth studies of anatomical, physiological properties [1], biochemical parameters [2][3] and plant morphology [4][5] have been carried out. Results of the variability of genotypic and phenotypic properties studying depending on the area were obtained using the example of some tree species [6][7][8][9]. A general overview of climatic variability is being formed: with an increase in the latitude of the area, the width, area of the leaf blade and the metabolic rate decrease.…”

Section: Introductionmentioning

confidence: 99%

Spatial Variability of Small-leaved Linden (Tilia cordata Mill.) Leaf Blade

et al. 2021

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The features of asymmetry and shape of small-leaved linden leaf blades (Tilia cordata Mill.) on the Kola Peninsula (Murmansk region, Kirovsk) and in the middle part of Russia (Moscow region, Orekhovo-Zuevo) were studied. The size of leaf blades in the northern population (Murmansk oblast) was significantly smaller (p <0.0001). Five linear traits had non-directional fluctuating asymmetry (FA). The method of geometric morphometrics showed a higher variability of leaf shape in the northern population compared to the southern one. Leaves differed in shape upon re-collection, and the leaf × side factor interaction showed the presence of FA in both populations (Goodall’s F criterion in the northern population was 31.7; in the southern – 92.3; p <0.0001). A high value of directional asymmetry was noted in the population on the Kola Peninsula (p <0.0001). The asymmetry of the shape did not depend on size of the leaf blades. According to linear features, plastic variability, especially in the northern population, strongly correlated with fluctuation variability (r = 0.81-0.82; p <0.05). The obtained data allows us to conclude that method of geometric morphometrics was more complex in determining the stability of development. FA was higher in the southern population, while directional asymmetry with a weak expropriation of FA was higher in the northern population. High latitude did not affect the decrease in developmental stability in terms of FA, but led to a decrease in the area of leaf blades with directional asymmetry.

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Leaf geometric morphometrics among populations of Dalbergia ecastaphyllum (L.) Taub.

Cited by 6 publications

References 16 publications

Geometry morphometrics of plant structures as a phenotypic tool to differentiate Polylepis incana Kunth. and Polylepis racemosa Ruiz & Pav. reforested jointly in Ecuador

Geometry morphometrics of plant structures as a phenotypic tool to differentiate Polylepis incana Kunth. and Polylepis racemosa Ruiz & Pav. reforested jointly in Ecuador

Leaf geometric morphometric analyses of Callicarpa and Geunsia (Lamiaceae) in the Malesian region

Spatial Variability of Small-leaved Linden (Tilia cordata Mill.) Leaf Blade

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