Leaf-color mutation induced by ethyl methane sulfonate and genetic and physio-biochemical characterization of leaf-color mutants in pepper (Capsicum annuum L.)

Cheng, Guo-Xin; Zhang, Rui-Xing; Liu, Shuai; He, Yu-Mei; Feng, Xiaohui; Haq, Saeed Ul; Luo, Da; Gong, Zhen-Hui

doi:10.1016/j.scienta.2019.108709

Cited by 6 publications

(3 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In Chl-deficient rice mutants, crucial PSI core proteins (psaA and psaB), subunits of ATP synthase, cytochrome, and the LHC, were significantly decreased [83]. The down-regulation of genes involved in photosynthesis impaired the function of these complexes, hindering photosynthesis [84]. Significant alterations in the phosphorylation levels of PsbC, PsbO, PsbP, LHCA2, and LHCA3 proteins were key factors contributing to the observed photosynthetic disparities between the yellowing mutant yl1 and the wild type [85].…”

Section: Discussionmentioning

confidence: 99%

Molecular Mechanisms of Chlorophyll Deficiency in Ilex × attenuata ‘Sunny Foster’ Mutant

Zou,

Huang,

Zhang

et al. 2024

Plants

View full text Add to dashboard Cite

Ilex × attenuata ‘Sunny Foster’ represents a yellow leaf mutant originating from I. × attenuata ‘Foster#2’, a popular ornamental woody cultivar. However, the molecular mechanisms underlying this leaf color mutation remain unclear. Using a comprehensive approach encompassing cytological, physiological, and transcriptomic methodologies, notable distinctions were discerned between the mutant specimen and its wild type. The mutant phenotype displayed aberrant chloroplast morphology, diminished chlorophyll content, heightened carotenoid/chlorophyll ratios, and a decelerated rate of plant development. Transcriptome analysis identified differentially expressed genes (DEGs) related to chlorophyll metabolism, carotenoid biosynthesis and photosynthesis. The up-regulation of CHLD and CHLI subunits leads to decreased magnesium chelatase activity, while the up-regulation of COX10 increases heme biosynthesis—both impair chlorophyll synthesis. Conversely, the down-regulation of HEMD hindered chlorophyll synthesis, and the up-regulation of SGR enhanced chlorophyll degradation, resulting in reduced chlorophyll content. Additionally, genes linked to carotenoid biosynthesis, flavonoid metabolism, and photosynthesis were significantly down-regulated. We also identified 311 putative differentially expressed transcription factors, including bHLHs and GLKs. These findings shed light on the molecular mechanisms underlying leaf color mutation in I. × attenuata ‘Sunny Foster’ and provide a substantial gene reservoir for enhancing leaf color through breeding techniques.

show abstract

Section: Discussionmentioning

confidence: 99%

Molecular Mechanisms of Chlorophyll Deficiency in Ilex × attenuata ‘Sunny Foster’ Mutant

Zou,

Huang,

Zhang

et al. 2024

Plants

View full text Add to dashboard Cite

show abstract

“…A series of white leaf color mutants virescen1, virescen3, and yellow-green leaf YSA mutants were found in rice, all of which were proved to be controlled by a pair of recessive nuclear genes [28][29][30]. In addition, leaf color mutations controlled by a single recessive gene were also found in such species as Capsicum annuum, Cucumis sativus, and C. melo [27,31,32]. Moreover, it was found that the Sesamum indicum yellow-green mutation was controlled by an incompletely dominant nuclear gene, Siyl-1 [33].…”

Section: Genetic Model Of Plant Leaf Color Mutantsmentioning

confidence: 98%

Mutation Mechanism of Leaf Color in Plants: A Review

Zhao

Zhang

et al. 2020

Forests

View full text Add to dashboard Cite

Color mutation is a common, easily identifiable phenomenon in higher plants. Color mutations usually affect the photosynthetic efficiency of plants, resulting in poor growth and economic losses. Therefore, leaf color mutants have been unwittingly eliminated in recent years. Recently, however, with the development of society, the application of leaf color mutants has become increasingly widespread. Leaf color mutants are ideal materials for studying pigment metabolism, chloroplast development and differentiation, photosynthesis and other pathways that could also provide important information for improving varietal selection. In this review, we summarize the research on leaf color mutants, such as the functions and mechanisms of leaf color mutant-related genes, which affect chlorophyll synthesis, chlorophyll degradation, chloroplast development and anthocyanin metabolism. We also summarize two common methods for mapping and cloning related leaf color mutation genes using Map-based cloning and RNA-seq, and we discuss the existing problems and propose future research directions for leaf color mutants, which provide a reference for the study and application of leaf color mutants in the future.

show abstract

“…Yan et al (2019) treated tomato seeds with EMS and obtained mutant plants with yellow leaves during the seed breeding process. Cheng et al (2019) used EMS to mutagenize pepper (Capsicum annuum L.) seeds and screened out the light green leaf mutants of pepper with ornamental value during the seed breeding process.…”

Section: Introductionmentioning

confidence: 99%

Analysis of Photosynthetic Pigment Content and Leaf Structure of Pteroceltis Tatarinowii Mutant with Yellow Leaf

Yan¹,

Lv²,

Liu³

et al. 2022

American Journal of Biochemistry and Biotechnology

View full text Add to dashboard Cite

The relationship between leaf variation characteristics and leaf structure characteristics of Pteroceltis tatarinowii with yellow leaf was explored to provide a theoretical basis for the breeding of other ornamental plants. In this study, the differences in pigment content, leaf structure, and chloroplast ultrastructure in leaves of Pteroceltis tatarinowii mutant with yellow leaf 'Huiguang' and its original species 'Lingyansi No. 1' were studied. The results showed that the chlorophyll in 'Huiguang' leaves decreased significantly, and the carotenoids-total chlorophyll ratio increased greatly. The palisade tissue cells in 'Huiguang' leaves are short, thick, and loosely structured, while the sponge tissue cells are irregular in shape and loosely arranged. In addition, the chloroplasts of 'Huiguang' leaves were irregular in shape and the grana lamellae were loosely arranged, broken, and missing, with more osmiophilic granules and fewer starch granules. These anomalies in leaf pigment content and chloroplast structure affect leaf photosynthesis and chlorophyll accumulation, thereby affecting leaf color changes.

show abstract

Leaf-color mutation induced by ethyl methane sulfonate and genetic and physio-biochemical characterization of leaf-color mutants in pepper (Capsicum annuum L.)

Cited by 6 publications

References 53 publications

Molecular Mechanisms of Chlorophyll Deficiency in Ilex × attenuata ‘Sunny Foster’ Mutant

Molecular Mechanisms of Chlorophyll Deficiency in Ilex × attenuata ‘Sunny Foster’ Mutant

Mutation Mechanism of Leaf Color in Plants: A Review

Analysis of Photosynthetic Pigment Content and Leaf Structure of Pteroceltis Tatarinowii Mutant with Yellow Leaf

Contact Info

Product

Resources

About