Leaf and Flower Extracts of Six <i>Michelia</i> L.: Polyphenolic Composition, Antioxidant, Antibacterial Activities and <i>in Vitro</i> Inhibition of α‐Amylase and α‐Glucosidase

Liao, Tianzhu; Cao, Jiankang; Yang, Zhenyu; Jin, Cheng; Lu, Jun

doi:10.1002/cbdv.202100894

Cited by 5 publications

(3 citation statements)

References 44 publications

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“…Among them, 7 compounds including nitidine, chelerythrine, sanguinarine, magnoflorine, allocryptopine, dictamnine, and palmatine have been compared with chemical reference standards. The other compounds were inferred by comparing their accurate quasi-molecular ions and fragment ions with information in the homemade database, which was constructed by searching for the data about ZN or its related plants. − The systematic error was found to be within ±7 ppm through comparison with chemical references. Specifically, 106 compounds were detected in the root, 72 compounds were detected in the stem, and 37 compounds were detected in the leaf.…”

Section: Resultsmentioning

confidence: 99%

Integration of High-Resolution LC-Q-TOF Mass Spectrometry and Multidimensional Chemical−Biological Analysis to Detect Nanomolar-Level Acetylcholinesterase Inhibitors from Different Parts of Zanthoxylum nitidum

Zhang,

Pei,

Wang

et al. 2024

J. Agric. Food Chem.

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Zanthoxyli radix is a popular tea among the elderly, and it is believed to have a positive effect on Alzheimer's disease. In this study, a highly effective three-step strategy was proposed for comprehensive analysis of the active components and biological functions of Zanthoxylum nitidum (ZN), including high-resolution LC-Q-TOF mass spectrometry (HRMS), multivariate statistical analysis for heterogeneity (MSAH), and experimental and virtual screening for bioactivity analysis (EVBA). A total of 117 compounds were identified from the root, stem, and leaf of ZN through HRMS. Bioactivity assays showed that the order of acetylcholinesterase (AChE) inhibitory activity from strong to weak was root > stem > leaf. Nitidine, chelerythrine, and sanguinarine were found to be the main differential components of root, stem, and leaf by OPLS-DA. The IC 50 values of the three compounds are 0.81 ± 0.02, 0.14 ± 0.01, and 0.48 ± 0.01 μM respectively, indicating that they are potent and high-quality AChE inhibitors. Molecular docking showed that pi-pi T-shaped interactions and pi-lone pairs played important roles in AChE inhibition. This study not only explains the biological function of Zanthoxyli radix in alleviating Alzheimer's disease to some extent, but also lays the foundation for the development of stem and leaf of ZN.

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Section: Resultsmentioning

confidence: 99%

Integration of High-Resolution LC-Q-TOF Mass Spectrometry and Multidimensional Chemical−Biological Analysis to Detect Nanomolar-Level Acetylcholinesterase Inhibitors from Different Parts of Zanthoxylum nitidum

Zhang,

Pei,

Wang

et al. 2024

J. Agric. Food Chem.

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“…Then 50 μL α-PNPG was added and incubated at 37°C for 20 min, and finally 100 μL Na 2 CO 3 (0.1 M) was added and incubated at 37°C for 10 min to terminate the reaction. The absorbance was measured at 405 nm ( Liao et al, 2022 ). The mixture without sample as the negative control, and acarbose as the positive control.…”

Section: Methodsmentioning

confidence: 99%

Exploring the diversity, bioactivity of endophytes, and metabolome in Synsepalum dulcificum

Liu,

Hou,

Zheng

et al. 2024

Front. Microbiol.

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Synsepalum dulcificum exhibits high edible and medicinal value; however, there have been no reports on the exploration of its endophyte resources. Here, we conducted analyses encompassing plant metabolomics, microbial diversity, and the biological activities of endophytic metabolites in S. dulcificum. High-throughput sequencing identified 4,913 endophytic fungal amplicon sequence variants (ASVs) and 1,703 endophytic bacterial ASVs from the roots, stems, leaves, flowers, and fruits of S. dulcificum. Fungi were classified into 5 phyla, 24 classes, 75 orders, 170 families, and 313 genera, while bacteria belonged to 21 phyla, 47 classes, 93 orders, 145 families, and 232 genera. Furthermore, there were significant differences in the composition and content of metabolites in different tissues of S. dulcificum. Spearman’s correlation analysis of the differential metabolites and endophytes revealed that the community composition of the endophytes correlated with plant-rich metabolites. The internal transcribed spacer sequences of 105 isolates were determined, and phylogenetic analyses revealed that these fungi were distributed into three phyla (Ascomycota, Basidiomycota, and Mucoromycota) and 20 genera. Moreover, 16S rDNA sequencing of 46 bacteria revealed they were distributed in 16 genera in three phyla: Actinobacteria, Proteobacteria, and Firmicutes. The antimicrobial activities (filter paper method) and antioxidant activity (DPPH and ABTS assays) of crude extracts obtained from 68 fungal and 20 bacterial strains cultured in different media were evaluated. Additionally, the α-glucosidase inhibitory activity of the fungal extracts was examined. The results showed that 88.6% of the strains exhibited antimicrobial activity, 55.7% exhibited antioxidant activity, and 85% of the fungi exhibited α-glucosidase inhibitory activity. The research suggested that the endophytes of S. dulcificum are highly diverse and have the potential to produce bioactive metabolites, providing abundant species resources for developing antibiotics, antioxidants and hypoglycemic drugs.

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“…Selain kayu, M. alba juga menghasilkan minyak atsiri bernilai tinggi yang digunakan sebagai topnote kunci pada parfum berharga mahal seperti Joy dan J'adore [7,8]. Beberapa spesies Michelia telah digunakan sebagai obat tradisional untuk berbagai penyakit seperti bronkitis, prostatitis, dan kanker [9][10][11]. M. alba diketahui menunjukkan aktivitas antijamur [12], antibakteri [13], dan telah digunakan dalam pengobatan demam, sifilis, gonorrhoea, malaria, dan aborsi [14].…”

Section: Pendahuluanunclassified

Penyelidikan Aktivitas Antikanker Payudara oleh Minyak Atsiri Bunga Michelia Alba Secara In Silico

Pratama¹,

Isman²,

Fadlan³

2022

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Kanker adalah penyakit mematikan di seluruh dunia. Salah satu jenis kanker dengan angka kejadian tinggi di Indonesia adalah kanker payudara. Secara umum kanker dapat ditangani dengan pembedahan, radioterapi, dan kemoterapi. Namun, ketiga teknik ini menunjukkan berbagai kelemahan dan efek samping yang merugikan. Bahan alam selanjutnya dapat menjadi alternatif penanganan kanker karena telah terbukti secara empiris dalam penanganan berbagai penyakit. Michelia alba dari keluarga Magnoliaceae menghasilkan minyak atsiri dan telah digunakan sebagai obat tradisional untuk bronkitis, prostatitis, demam, sifilis, gonorrhoea, malaria, dan kanker. Meskipun demikian, kajian yang mengungkap potensi M. alba dalam penanganan kanker tidak banyak dilaporkan dan sangat terbatas. Berdasarkan hal tersebut, penelitian ini bertujuan untuk menyelidiki potensi aktivitas antikanker payudara minyak atsiri bunga M. alba secara in silico. Penelitian dilakukan dengan docking molekular senyawa minyak atsiri bunga M. alba terhadap human epidermal growth factor receptor 2 (identitas PDB 3PP0). Penelitian juga dilakukan dengan analisis sifat fisikokimia dan farmakokinetika senyawa minyak atsiri M. alba. Hasil docking molekular menunjukkan α-terpineol, geraniol, β-pinen, limonen, linalool, β-fenil etilalkohol, dan 1,8-cineol memiliki skor docking yang lebih rendah dari kontrol kuersetin. Analisis fisikokimia dan farmakokinetika mengungkap bahwa senyawa minyak atsiri M. alba tidak melanggar aturan Lipinski dan Veber serta memiliki profil penyerapan, distribusi, metabolisme, ekskresi, dan toksisitas yang baik.

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Leaf and Flower Extracts of Six Michelia L.: Polyphenolic Composition, Antioxidant, Antibacterial Activities and in Vitro Inhibition of α‐Amylase and α‐Glucosidase

Cited by 5 publications

References 44 publications

Integration of High-Resolution LC-Q-TOF Mass Spectrometry and Multidimensional Chemical−Biological Analysis to Detect Nanomolar-Level Acetylcholinesterase Inhibitors from Different Parts of Zanthoxylum nitidum

Integration of High-Resolution LC-Q-TOF Mass Spectrometry and Multidimensional Chemical−Biological Analysis to Detect Nanomolar-Level Acetylcholinesterase Inhibitors from Different Parts of Zanthoxylum nitidum

Exploring the diversity, bioactivity of endophytes, and metabolome in Synsepalum dulcificum

Penyelidikan Aktivitas Antikanker Payudara oleh Minyak Atsiri Bunga Michelia Alba Secara In Silico

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