Leaf and Canopy Traits Associated with Stay-Green Expression Are Closely Related to Yield Components of Wheat Genotypes with Contrasting Tolerance to Water Stress

Moraga, Felipe; Alcaíno, Marta; Matus, Iván; Castillo, Dalma; Pozo, Alejandro del

doi:10.3390/plants11030292

Cited by 8 publications

(4 citation statements)

References 41 publications

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“…Canopy NDVI results indicated that senescence began prematurely due to water limitation (Moraga et al 2022). This shortening of the life cycle (shorter grain filling periods) and/or decreased ability of source organs to supply nutrients may have been associated with the lower yields under rainfed conditions (De Santis et al 2021).…”

Section: Water Stress Reduced Crop Yield and Biomass And Altered Grai...mentioning

confidence: 99%

Analysis of durum wheat photosynthetic organs during grain filling reveals the ear as a water stress-tolerant organ and the peduncle as the largest pool of primary metabolites

Martínez-Peña

Vergara-Díaz

Schlereth

et al. 2023

Planta

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Main conclusion The pool of carbon- and nitrogen-rich metabolites is quantitatively relevant in non-foliar photosynthetic organs during grain filling, which have a better response to water limitation than flag leaves. Abstract The response of durum wheat to contrasting water regimes has been extensively studied at leaf and agronomic level in previous studies, but the water stress effects on source–sink dynamics, particularly non-foliar photosynthetic organs, is more limited. Our study aims to investigate the response of different photosynthetic organs to water stress and to quantify the pool of carbon and nitrogen metabolites available for grain filling. Five durum wheat varieties were grown in field trials in the Spanish region of Castile and León under irrigated and rainfed conditions. Water stress led to a significant decrease in yield, biomass, and carbon and nitrogen assimilation, improved water use efficiency, and modified grain quality traits in the five varieties. The pool of carbon (glucose, glucose-6-phosphate, fructose, sucrose, starch, and malate) and nitrogen (glutamate, amino acids, proteins and chlorophylls) metabolites in leaf blades and sheaths, peduncles, awns, glumes and lemmas were also analysed. The results showed that the metabolism of the blades and peduncles was the most susceptible to water stress, while ear metabolism showed higher stability, particularly at mid-grain filling. Interestingly, the total metabolite content per organ highlighted that a large source of nutrients, which may be directly involved in grain filling, are found outside the blades, with the peduncles being quantitatively the most relevant. We conclude that yield improvements in our Mediterranean agro-ecosystem are highly linked to the success of shoots in producing ears and a higher number of grains, while grain filling is highly dependent on the capacity of non-foliar organs to fix CO2 and N. The ear organs show higher stress resilience than other organs, which deserves our attention in future breeding programmes.

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Section: Water Stress Reduced Crop Yield and Biomass And Altered Grai...mentioning

confidence: 99%

Analysis of durum wheat photosynthetic organs during grain filling reveals the ear as a water stress-tolerant organ and the peduncle as the largest pool of primary metabolites

Martínez-Peña

Vergara-Díaz

Schlereth

et al. 2023

Planta

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“…In order to achieve this goal, researchers used a combination of traditional phenotypic data and SSR markers to analyze the genetic diversity of candidate genotypes [7,[23][24][25]. Nevertheless, the use of high-throughput-origin indirect phenotypic data along with the SSR markers to explore wheat genetic diversity is scant [26,27]. Keeping this lacuna in mind, we used canopy-reflectance-based vegetation indices and yield parameters, as well as 30 SSR markers, to assess the genetic diversity of 56 bread-wheat genotypes under irrigated control and irrigation-simulated dry situations.…”

Section: Introductionmentioning

confidence: 99%

Assessment of Genetic Diversity of Bread Wheat Genotypes for Drought Tolerance Using Canopy Reflectance-Based Phenotyping and SSR Marker-Based Genotyping

et al. 2022

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This study investigated the genetic diversity of bread-wheat genotypes using canopy reflectance-based vegetation indices (VIs) and simple sequence repeat (SSR) marker-based genotyping for drought tolerance. A total of 56 wheat genotypes were assessed using phenotypic traits (combination of VIs and yield traits) and 30 SSR markers. The data of the phenotypic traits were averaged over two growing seasons under irrigated and drought-stressed conditions. The hierarchical clustering of the wheat genotypes unveiled three drought-tolerant groups. Cluster 1 genotypes showed minimal phenotypic alterations, conferring superior drought tolerance and yield stability than clusters 2 and 3. The polymorphism information content values for the SSR markers ranged from 0.434 to 0.932, averaging 0.83. A total of 458 alleles (18.32 alleles per locus) were detected, with the most polymorphic markers, wmc177 and wms292, having the most alleles (24). A comparative study of SSR diversity among phenotypic clusters indicated that genotypes under cluster 1 had higher genetic diversity (0.879) and unique alleles (47%), suggesting their potential in future breeding programs. The unweighted neighbor-joining tree grouped the wheat genotypes into five major clusters. Wheat genotypes from all phenotypic clusters were distributed throughout all SSR-based clusters, indicating that genetically heterogeneous genotypes were allocated to different drought-tolerant groups. However, SSR-based clusters and model-based populations showed significant co-linearity (86.7%). The findings of the present study suggest that combining reflectance-based indirect phenotyping with SSR-based genotyping might be an effective technique for assessing genetic diversity to improve the drought tolerance of bread-wheat genotypes.

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“…Maize is the largest grain crop planted in China and is an important source of food and industrial raw material. The essence of crop yield formation is the process of the sourcesink flow interaction, and the increase in the yield of stay-green crops is directly related to the maintenance of their photosynthetic capacity during the grain-filling period [25][26][27]. Determining the genetic basis of stay-green molecules is beneficial for cultivating new varieties of ideal plant types through molecular breeding methods, thereby comprehensively improving crop resistance to adverse conditions and improving their feeding value and yield potential.…”

Section: Excavation Of Key Genes In the Stay-green Mutant Contributes...mentioning

confidence: 99%

Combined BSA-Seq and RNA-Seq Reveal Genes Associated with the Visual Stay-Green of Maize (Zea mays L.)

Zheng,

Deng,

et al. 2023

IJMS

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Maize has become one of the most widely grown grains in the world, and the stay-green mutant allows these plants to maintain their green leaves and photosynthetic potential for longer following anthesis than in non-mutated plants. As a result, stay-green plants have a higher production rate than non-stay-green varieties due to their prolonged grain-filling period. In this study, the candidate genes related to the visual stay-green at the maturation stage of maize were investigated. The F2 population was derived from the T01 (stay-green) and the Xin3 (non-stay-green) cross. Two bulked segregant analysis pools were constructed. According to the method of combining ED (Euclidean distance), Ridit (relative to an identified distribution unit), SmoothG, and SNP algorithms, a region containing 778 genes on chromosome 9 was recognized as the candidate region associated with the visual stay-green in maize. A total of eight modules were identified using WGCNA (weighted correlation network analysis), of which green, brown, pink, and salmon modules were significantly correlated with visual stay-green. BSA, combined with the annotation function, discovered 7 potential candidate genes, while WGCNA discovered 11 stay-green potential candidate genes. The candidate range was further reduced due through association analysis of BSA-seq and RNA-seq. We identified Zm00001eb378880, Zm00001eb383680, and Zm00001eb384100 to be the most likely candidate genes. Our results provide valuable insights into this new germplasm resource with reference to increasing the yield for maize.

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Leaf and Canopy Traits Associated with Stay-Green Expression Are Closely Related to Yield Components of Wheat Genotypes with Contrasting Tolerance to Water Stress

Cited by 8 publications

References 41 publications

Analysis of durum wheat photosynthetic organs during grain filling reveals the ear as a water stress-tolerant organ and the peduncle as the largest pool of primary metabolites

Analysis of durum wheat photosynthetic organs during grain filling reveals the ear as a water stress-tolerant organ and the peduncle as the largest pool of primary metabolites

Assessment of Genetic Diversity of Bread Wheat Genotypes for Drought Tolerance Using Canopy Reflectance-Based Phenotyping and SSR Marker-Based Genotyping

Combined BSA-Seq and RNA-Seq Reveal Genes Associated with the Visual Stay-Green of Maize (Zea mays L.)

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