Leaf anatomy and chlorophyll content in Psychotria vellosiana Benth influenced by the edge and two matrices

Abstract

The distance effect of the edge and two matrices (pasture and sugar cane) on the microclimate and anatomical and physiological properties of Psychotria vellosiana leaves were studied in two semideciduous forest fragments in southern Minas Gerais. The plots were set at distances (0, 20, 40, 60 and 80 m) from the edge to the interior of each fragment: 0, 20 and 40 m distances were considered edge, whereas 60 and 80 m were considered interior. Microclimate data were collected in all plots and radiation data and canopy openness at 0, 40 and 80 m. Leaves were collected for analysis of their chlorophyll content and anatomical properties. No differences were found for the canopy openness and radiation between the edge and interior of the fragments. The temperature and humidity data and the soil humidity displayed no differences within or between each fragment. Only the soil temperature data differed between the fragments. The leaves displayed the same chlorophyll content for the edge and interior of the fragments and between the fragments. These results may be related to the microclimate properties found along the 80 m transect in each fragment. Some anatomical data suggested a better adaptation of P. vellosiana to the fragment surrounded by the pasture matrix. However, we require a more detailed analysis that includes nutrient information and water availability from the soil. RESUMO: O efeito da distância da borda e de duas matrizes (pastagem e cana-de-açúcar) sobre o microclima e as características anatômicas e fisiológicas de folhas de Psychotria vellosiana foi estudado em dois fragmentos de floresta estacional semidecidual no sul de Minas Gerais. As parcelas foram estabelecidas em distâncias-0, 20, 40, 60 e 80 m-a partir da borda para o interior de cada fragmento. As distâncias de 0, 20 e 40 m foram consideradas como borda e de 60 e 80 m, como interior. Em todas as parcelas, foram coletados dados microclimáticos e, nas parcelas de 0, 40 e 80 m, dados de radiação e abertura de dossel. Folhas foram coletadas para análises de teor de clorofila e características anatômicas. Não houve diferença em relação à abertura do dossel e à radiação entre borda e interior de fragmentos. Os dados de temperatura e umidade do ar, e umidade do solo não apresentaram diferenças dentro dos fragmentos e entre estes. Somente os dados de temperatura do solo variaram entre os fragmentos. As folhas apresentaram o mesmo teor de clorofila para borda e interior de fragmentos, e entre os fragmentos. Estes resultados podem estar relacionados às características microclimáticas encontradas ao longo dos 80 m em cada fragmento. Alguns dados anatômicos sugerem melhor adaptação de P. vellosiana no fragmento circundado por matriz de pasto. No entanto, é necessária uma análise mais detalhada, incluindo a disponibilidade de água e nutrientes do solo. Leaf anatomy and chlorophyll content in Psychotria vellosiana Benth influenced by the edge and two matrices Anatomia foliar e teor de clorofila em Psychotria vellosiana Benth influenciados pela matriz e pela di...

The detection of Escherichia coli biofilm in green oak with spectroscopic imaging techniques based on confocal laser scanning microscope

“…The autofluorescence signal of stomata area was stronger than another area related to chlorophyll content. Nayara in 2013 [ 9] reported the leaves area with high stomatal index and stomatal density (stomata/mm 2 ) will induce the high chlorophyll .Morever ,chlorophyll level depends on the stomatal conductance. Sami et al [10] describe the effect of stomata on chlorophyll content in the leaf.…”

“…The spectral signal at 680-690 nm represented the chloroplast with chlorophyll A. The Chlorophyll A was commonly existed in green plants and emitted at wavelength 688 and 730 nm after being excited by blue-green fluorescence [7][8][9]. Therefore, the spectral wavelength of 620 and 670 nm can be used as unambiguous indicators for either E. coli or its' biofilm.…”