Lead (Pb) Exposure Enhances Expression of Factors Associated with Inflammation

Metryka, Emilia; Chibowska, Karina; Gutowska, Izabela; Falkowska, Anna; Kupnicka, Patrycja; Barczak, Katarzyna; Chlubek, Dariusz; Baranowska-Bosiacka, Irena

doi:10.3390/ijms19061813

Cited by 141 publications

(81 citation statements)

References 121 publications

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“…Ranked the second most toxic substance on the priority list created by the Agency for Toxic Substances and Disease Registry (ATSDR) in 2017 [6], Pb poses a particular risk to the nervous system, especially in early life [7][8][9][10][11]. It is linked to numerous metabolic and structural changes in the developing brain (for detailed review see [12][13][14][15]), resulting in various cognitive disorders [16][17][18][19]. Children exposed to Pb in the early stages of development show a high incidence of many neurodevelopmental diseases, such as hyperactivity, emotional symptoms and conduct disorder [20], attention-deficit hyperactivity disorder (ADHD) [21,22], autism spectrum disorder (ASD) [23][24][25][26], and tend to have lower IQs [27,28].…”

Section: Introductionmentioning

confidence: 99%

Pre- and Neonatal Exposure to Lead (Pb) Induces Neuroinflammation in the Forebrain Cortex, Hippocampus and Cerebellum of Rat Pups

Chibowska

Korbecki

Gutowska

et al. 2020

IJMS

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Lead (Pb) is a heavy metal with a proven neurotoxic effect. Exposure is particularly dangerous to the developing brain in the pre-and neonatal periods. One postulated mechanism of its neurotoxicity is induction of inflammation. This study analyzed the effect of exposure of rat pups to Pb during periods of brain development on the concentrations of selected cytokines and prostanoids in the forebrain cortex, hippocampus and cerebellum. Methods: Administration of 0.1% lead acetate (PbAc) in drinking water ad libitum, from the first day of gestation to postnatal day 21, resulted in blood Pb in rat pups reaching levels below the threshold considered safe for humans by the Centers for Disease Control and Prevention (10 µg/dL). Enzyme-linked immunosorbent assay (ELISA) method was used to determine the levels of interleukins IL-1β, IL-6, transforming growth factor-β (TGF-β), prostaglandin E2 (PGE2) and thromboxane B2 (TXB2). Western blot and quantitative real-time PCR were used to determine the expression levels of cyclooxygenases COX-1 and COX-2. Finally, Western blot was used to determine the level of nuclear factor kappa B (NF-κB). Results: In all studied brain structures (forebrain cortex, hippocampus and cerebellum), the administration of Pb caused a significant increase in all studied cytokines and prostanoids (IL-1β, IL-6, TGF-β, PGE2 and TXB2). The protein and mRNA expression of COX-1 and COX-2 increased in all studied brain structures, as did NF-κB expression. Conclusions: Chronic pre-and neonatal exposure to Pb induces neuroinflammation in the forebrain cortex, hippocampus and cerebellum of rat pups.

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Section: Introductionmentioning

confidence: 99%

Pre- and Neonatal Exposure to Lead (Pb) Induces Neuroinflammation in the Forebrain Cortex, Hippocampus and Cerebellum of Rat Pups

Chibowska

Korbecki

Gutowska

et al. 2020

IJMS

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“…Occupational and environmental exposure to metals and metalloids can cause many pathological conditions, including immunotoxicity. Research has shown that the most important mechanism underlying diseases associated with lead exposure is inflammation (1)(2)(3). Inflammation also plays a major role in the development of atherosclerosis (4,5).…”

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confidence: 99%

“…Numerous scientific studies have shown the link between lead exposure and cardiovascular abnormalities such as coronary artery disease, atherosclerosis, stroke, peripheral arterial disease, and arterial hypertension (11)(12)(13)(14). Many have also established that lead exposure is closely related to inflammation (2,15,16) and that it increases pro-inflammatory cytokine production, including tumour necrosis factor-α (TNF-α), interleukin-6 (IL-6), and interleukin-10 (IL-10) (17,18).…”

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confidence: 99%

The effects of occupational lead exposure on selected inflammatory biomarkers

Türksoy

Tutkun

İritaş

et al. 2019

Archives of Industrial Hygiene and Toxicology

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In exposure to toxic metals such as lead, determining lead and cytokine levels (IL-6, IL-10, and TNF-α) is essential for early detection of diseases. The aim of this study was to develop an model for early detection of inflammation and onset of atherosclerosis in the absence of clinical findings in young workers, which could help physicians take timely an action and start treatment. This study included 49 metal workers exposed to lead occupationally and 50 unexposed administrative workers (controls) who underwent immunological analysis for cytokines (IL-6, IL-10, and TNF-α) and atherosclerosis markers (h-FABP and VCAM-1), toxicological analysis for lead, and routine biochemical analysis (ALT, AST, creatinine) at the Ankara Occupational and Environmental Diseases Hospital in 2017. Lead levels correlated with IL-6, IL-10, and TNF-α (r=0.469, r=0.521 and r=0.279, respectively, p<0.01) but did not significantly affect h-FABP and VCAM-1 levels.

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“…THP-1 macrophages were pretreated with the compounds at 10, 20 and 50 μM for 1 h, and then stimulated with LPS (1 μg/mL) for 24 h. As shown in Figure 4, LPS induced a significant increase in the production of TNF-α, IL-6 and IL-1β in THP-1 cells in relation to unstimulated control cells (p < 0.001). These molecules are potent pro-inflammatory cytokines produced by immune cells at the site of inflammation, and play a crucial role in local and systemic inflammatory responses [16]. Dexamethasone, used as an anti-inflammatory reference drug, strongly inhibited LPS-induced TNF-α, IL-6, and IL-1β production.…”

Section: Anti-inflammatory Activitymentioning

confidence: 99%

New Eunicellin-Type Diterpenes from the Panamanian Octocoral Briareum asbestinum

et al. 2020

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Gorgonian octocorals are considered a prolific source of secondary metabolites with a wide range of biological activities, including anti-inflammatory activity. In particular, the genus Briareum is known for producing a wealth of diterpenes with complex chemical structures. The chemical study of the methanolic extract of Briareum asbestinum collected in Bocas del Toro, on the Caribbean side of Panama, led to the isolation of three new eunicellin-type diterpenes: briarellin T (1), asbestinin 27 (2), asbestinin 28 (3) and the previously described asbestinin 17 (4). The structures of the new compounds were determined by extensive NMR analyses and HRMS. Anti-inflammatory activity assays showed a significant reduction of the pro-inflammatory cytokines TNF-α, IL-6, IL-1β and IL-8 as well as a downregulation of COX-2 expression in LPS-stimulated THP-1 macrophages. These findings support the potential use of these marine compounds as therapeutic agents in the treatment of inflammatory diseases.

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Lead (Pb) Exposure Enhances Expression of Factors Associated with Inflammation

Cited by 141 publications

References 121 publications

Pre- and Neonatal Exposure to Lead (Pb) Induces Neuroinflammation in the Forebrain Cortex, Hippocampus and Cerebellum of Rat Pups

Pre- and Neonatal Exposure to Lead (Pb) Induces Neuroinflammation in the Forebrain Cortex, Hippocampus and Cerebellum of Rat Pups

The effects of occupational lead exposure on selected inflammatory biomarkers

New Eunicellin-Type Diterpenes from the Panamanian Octocoral Briareum asbestinum

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