Lead induced DNA strand breaks in lymphocytes of exposed workers: role of reactive oxygen species and protein kinase C

Fracasso, Maria Enrica; Perbellini, Luigi; Soldà, Silvia; Talamini, Giorgio; Franceschetti, Paola

doi:10.1016/s1383-5718(02)00012-8

Cited by 126 publications

(60 citation statements)

References 35 publications

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“…calcium, magnesium, iron and zinc) with consequent effects on enzymes containing SH groups (Jennette, 1981), increased incorporation of erroneous nucleotides (Johnson, 1998) and the accumulation of free radicals due to alteration of the oxidative processes of cells and effects on repair mechanisms in which Genetics and Molecular Biology, 27, 1, 103-107 (2004) Copyright by the Brazilian Society of Genetics. Printed in Brazil www.sbg.org.br lead has been implicated as a co-carcinogen (Fracasso et al, 2002). This paper reports the results a study of the neotropical fish Hoplias malabaricus in which this fish was exposed to TBT and PbII and the effects of the exposure assessed using the comet assay and the piscine micronucleus and chromosome aberration tests.…”

Section: Introductionmentioning

confidence: 98%

Mutagenic effects of tributyltin and inorganic lead (Pb II) on the fish H. malabaricus as evaluated using the comet assay and the piscine micronucleus and chromosome aberration tests

et al. 2004

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Genotoxicity studies on toxic metals and their organic compounds are very important, especially so in the investigation of the effects of these compounds on the aquatic environments where they tend to accumulate. The use of endemic aquatic organisms as biological sentinels has proved useful to environmental monitoring. We assessed the mutagenic potential of tributyltin (TBT) and inorganic lead (PbII) using samples of the fish Hoplias malabaricus (commonly called traíra) using the comet assay and the piscine micronucleus and chromosome aberration tests. Eighteen H. malabaricus were acclimatized in three individual aquariums, each containing six fish, six fish being exposed to 0.3 µg/g of body weight (bw) of TBT, six to 21 µg/g bw of PbII and six being used as controls. Exposure to TBT and PbII was achieved by feeding the fish every five days with Astyanax (a small fish that is part of the normal diet of H. malabaricus) which had been injected with solutions of TBT, PbII or with water (the control group). After two months the H. malabaricus were sacrificed and their peripheral blood collected and subjected to the comet and micronucleus assays, the chromosome aberration assay being conducted using kidney-tissue. Although the comet assay showed now mutagenic effects at the lead concentrations used but encountered results with TBT, the micronucleus and chromosome aberrations assays both indicated that TBT and PbII are potentially mutagenic (p < 0.01), the micronucleus assay showing morphological alterations of the nucleus.

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Section: Introductionmentioning

confidence: 98%

Mutagenic effects of tributyltin and inorganic lead (Pb II) on the fish H. malabaricus as evaluated using the comet assay and the piscine micronucleus and chromosome aberration tests

et al. 2004

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“…It is likely that the Pb-related depletion of glutathione (GSH) and/or increased production of reactive oxygen species (ROS) may be the trigger in the induction of oxidative stress that causes specifi c responses, depending also on the functional cellular characteristics. The lymphocytes of Pb-exposed workers seem to respond to a chronic state of oxidative stress by mechanisms that involve downregulation of protein kinase Cα, an effect that is commonly seen with other tumor promoters (16). Evaluating the possible role of environmental exposure to Pb as a risk factor for prostate pathology in men suffering from prostate cancer and benign prostate hyperplasia (BPH), Siddiqui et al (17) found that BPb was signifi cantly higher in prostate cancer and BPH cases than in control subjects.…”

mentioning

confidence: 99%

Prostate-Specific Antigen (PSA) in Serum in Relation to Blood Lead Concentration and Alcohol Consumption in Men

Pizent

Čolak²,

Kljaković

et al. 2009

Archives of Industrial Hygiene and Toxicology

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The combined infl uence of age, smoking, alcohol, blood lead and cadmium concentrations, and serum copper, zinc, and selenium concentrations on prostate-specifi c antigen (PSA) in serum was investigated in a group of 57 men aged 21 years to 40 years. The subjects had no occupational exposure to metals and no other known reasons suspected of infl uencing prostate function or metal metabolism. No signifi cant correlation was found between PSA and any of the explanatory variables considered. Nevertheless, when taking into account all of the above-mentioned potentially explanatory variables, the results of stepwise multiple regression showed a signifi cant increase in PSA with respect to an increase in blood lead, and a decrease in PSA with respect to an increase in intensity of alcohol consumption. The median and range values of blood lead concentration in the 57 men were 26.0 µg L -1 and (10.1 to 108.0) µg L -1 , respectively. These results suggest that even a low-level environmental lead exposure, common for general populations worldwide, may contribute to the risk of prostate cancer in men. The infl uence of lead as well as cadmium, zinc, and selenium on prostate damage and PSA should be further investigated in relatively young men for the purpose of disease prevention.

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“…For example, battery plant workers (n=37) had significantly elevated levels of DNA breaks in lymphocytes compared to unexposed subjects (n=29) (Fracasso et al 2002). Moreover, the authors found significant correlations between DNA breaks and increased production of reactive oxygen species (ROS) and decreased glutathione levels in the lymphocytes, pointing to oxidative stress as a possible cause for the specific responses.…”

Section: Health Effectsmentioning

confidence: 96%

Characterizing Golden Eagle Risk to Lead and Anticoagulant Rodenticide Exposure: A Review

Herring

Eagles‐Smith

Buck

2017

Journal of Raptor Research

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Lead induced DNA strand breaks in lymphocytes of exposed workers: role of reactive oxygen species and protein kinase C

Cited by 126 publications

References 35 publications

Mutagenic effects of tributyltin and inorganic lead (Pb II) on the fish H. malabaricus as evaluated using the comet assay and the piscine micronucleus and chromosome aberration tests

Mutagenic effects of tributyltin and inorganic lead (Pb II) on the fish H. malabaricus as evaluated using the comet assay and the piscine micronucleus and chromosome aberration tests

Prostate-Specific Antigen (PSA) in Serum in Relation to Blood Lead Concentration and Alcohol Consumption in Men

Characterizing Golden Eagle Risk to Lead and Anticoagulant Rodenticide Exposure: A Review

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