Lead Exposure Induces Weight Gain in Adult Rats, Accompanied by DNA Hypermethylation

Sun, Honglin; Wang, Ningjian; Nie, Xiaomin; Zhao, Li; Li, Qin; Cang, Zhen; Chen, Chi; Lü, Meng; Cheng, Jing; Zhai, Hualing; Xia, Fangzhen; Ye, Lin; Lu, Yingli

doi:10.1371/journal.pone.0169958

Cited by 38 publications

(21 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This study suggests that combination of all solanum malongen, carrots subsp.sativus and solanum lycopersicon is suspected to properly reverse internal abnormality or injury resulting in weight loss in lead induced toxicity but may not properly normal the blood glucose level. Hence, in case of where hyperglycemia treatment is done using standard drugs, the administration of all three juices extract will help heal internal injuries but where injury is not yet sustained just insulin resistance, carrots subsp.sativus and solanum lycopersicon juice extract will be best for regulation of such lead induced hyperglycemia (Sun et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

Blood glucose lowering effect of Solanum melongena (garden egg), Solanum lycopersicum (tomatoes), Daucus carota subsp. Sativus (carrot) extracts on lead induced toxicity in albino wistar rats

Ip¹,

Ka²,

Amaechi³

2021

GSC Biol. and Pharm. Sci.

View full text Add to dashboard Cite

Lead is one of the heavy metal humans are often expose to either through food, cosmetics or environment. This study is aimed at investigating the positive effect of Solanum melongena (garden egg), Solanum lycopersicum (tomatoes) and Daucus carrots subsp.sativus (carrot) on blood glucose concentration, body weight, and feeding habits of albino rats on lead toxicity. In this study 35 albino rats weighing 80-120g were grouped into five. Group 1 served as normal control, group 2 negative control. The remaining three groups served as treatment groups 1, 2 and 3. All groups except group 1 (normal control) were administered 50mg/kg of lead acetate. Treatment groups were administered 200mg/kg of Solanum melongena (garden egg), Solanum lycopersicum (tomatoes) and Daucus carrots subsp.sativus (carrot). Group 3 was administered Solanum malongen and Daucus carrots subsp.sativus while group 4 was given Daucus carrots subsp.sativus and solanum lycopersicon. Group 5 was administered Solanum malongen, Daucus carrots subsp.sativus and Solanum lycopersicon. Changes in blood glucose concentration, body weight, feed and water consumption recorded at intervals. The result showed a significant decrease (P<0.05 and P<0.01) in blood glucose concentration in treatment groups compared to negative control. There was also significant increase (P>0.05, P>0.01 and P>0.001) in body weight and feed consumed in treatment groups compared to negative control. Combined therapy of any two of Solanum malongen, Daucus carrots subsp.sativus and Solanum lycopersicon juice can help in the management of hyperglycemia and reverse internal abnormality or injury resulting in weight loss in lead induced toxicity.

show abstract

Section: Discussionmentioning

confidence: 99%

Blood glucose lowering effect of Solanum melongena (garden egg), Solanum lycopersicum (tomatoes), Daucus carota subsp. Sativus (carrot) extracts on lead induced toxicity in albino wistar rats

Ip¹,

Ka²,

Amaechi³

2021

GSC Biol. and Pharm. Sci.

View full text Add to dashboard Cite

show abstract

“…Notably, changes in weight gain, glucose intolerance and insulin resistance in the STD+500 ppm Pb animals approximated those observed in animals fed a HFD only. No significant changes in hyperglycemia were observed with Pb exposure(53). Whole genome bisulfite sequencing of liver tissue from STD+500 ppm Pb animals revealed hypermethylation at the whole genome level and differentially methylated genes were enriched in pathways involved in lipid and glucose metabolism.…”

Section: Lead (Pb)mentioning

confidence: 95%

“…Cultured islets of Langerhans exposed to Pb had decreased cell viability and glucose-stimulated insulin secretion as well as altered markers of endoplasmic reticulum stress (e.g., glucose regulated protein 78 (Grp78), nuclear factor kappa-light-chain-enhancer of activated B cells (Nfkb), and CCAAT/enhancer-binding protein homologous protein (Chop), and increased baseline insulin levels, glycogen synthase kinase 3 beta ( Gsk3b ) activity, and ROS production. In another study, adult male rats (8 weeks) were exposed for 21 weeks to a STD without or with Pb (500, 1500 or 4500 ppm) administered via drinking water, or to HFD alone(53). STD+500 ppm Pb animals exhibited significantly greater weight gain, as well as fasting glucose and insulin levels, HOMA-IR, TGs and glucose intolerance compared to all other STD±Pb animals.…”

Section: Lead (Pb)mentioning

confidence: 99%

Heavy Metal Exposure and Metabolic Syndrome: Evidence from Human and Model System Studies

Planchart

Green

Hoyo

et al. 2018

Curr Envir Health Rpt

128

View full text Add to dashboard Cite

Data from 36 epidemiological studies involving 17 unique countries/regions and 13 studies leveraging model systems are included in this review. Epidemiological and model system studies support a possible association between heavy metal exposure and MS or comorbid conditions; however, results remain conflicting. Epidemiological studies were predominantly cross-sectional and collectively, they highlight a global interest in this question and reveal evidence of differential susceptibility by sex and age to heavy metal exposures. In vivo studies in rats and mice and in vitro cell-based assays provide insights into potential mechanisms of action relevant to MS including altered regulation of lipid and glucose homeostasis, adipogenesis, and oxidative stress. Heavy metal exposure may contribute to MS or comorbid conditions; however, available data are conflicting. Causal inference remains challenging as epidemiological data are largely cross-sectional; and variation in study design, including samples used for heavy metal measurements, age of subjects at which MS outcomes are measured; the scope and treatment of confounding factors; and the population demographics vary widely. Prospective studies, standardization or increased consistency across study designs and reporting, and consideration of molecular mechanisms informed by model system studies are needed to better assess potential causal links between heavy metal exposure and MS.

show abstract

“…It is known that heavy metals, including lead, are endocrine disrupters and also confirmed as being associated with obesity, which may indicate, along with environmental factors, the growing prevalence of overweight people (Grun and Blumberg 2006). The study by Sun et al (2017) demonstrated that chronic exposure to 0.05% lead (Pb) results in weight gain and insulin-specific resistance causing disorders are related to the metabolism.…”

Section: Analyzes Of the Animal Model 321 Evaluation Of Parametersmentioning

confidence: 99%

Evaluation of the Effects of Fluoride and Associated With Lead in Animal Model and Physical-Chemical Analysis of Public Supply Water and of the Sinos River in the South of Brazil

Krützmann

Machado

Constante

et al. 2020

Int J Innov Educ Res

View full text Add to dashboard Cite

Fluoride related to caries prevention is at the center of a scientific controversy. Studies show that fluoride causes damage to health and the environment, as well as reducing IQ in children. The fluoridation of drinking water, mandatory in Brazil, has repercussions over the whole society. One of our objectives was to know the concentrations of fluoride (F) and toxic metals of Sinos River, treated water and final consumers of the cities of Campo Bom (CB), Novo Hamburgo (NH) and São Leopoldo (SL), as well as the groundwater from Ivoti, located in southern Brazil. We also evaluated in rats the effects of F and, in association with lead (Pb), on thyroid hormones and the Total Antioxidant Capacity (TAC). Three groups of rats were exposed to different waters: G1-Control with distilled water (DW); G2-DW with 25ppm (F); G3-DW with 25ppm (F) + 30 ppm (Pb). The Sinos River has an average concentration of 0.0735 mg.L-1 of F. But the F of both the water treated by the ETA of SL as well as in the final consumers of SL had concentrations above 0.9 mg.L-1 (State Ordinance No. 10/1999). In addition, we verified the presence of Pb and Cr (VI) in all types of water. The results with the animals showed a significant difference in T3 (p=0.032) and in T4 (p=0.043) from G3 to G1. In TAC, the difference was significant from G2 to G1 and G3 (p=0.007), showing that F and F with Pb interfere with the endocrine and antioxidant functions of rats. In addition, the fact that there are water fluoridation failures shows that the population is exposed to health risks. We confirm that drinking water fluoridation needs to be demystified and reconsidered as a public health intervention.

show abstract

Lead Exposure Induces Weight Gain in Adult Rats, Accompanied by DNA Hypermethylation

Cited by 38 publications

References 36 publications

Blood glucose lowering effect of Solanum melongena (garden egg), Solanum lycopersicum (tomatoes), Daucus carota subsp. Sativus (carrot) extracts on lead induced toxicity in albino wistar rats

Blood glucose lowering effect of Solanum melongena (garden egg), Solanum lycopersicum (tomatoes), Daucus carota subsp. Sativus (carrot) extracts on lead induced toxicity in albino wistar rats

Heavy Metal Exposure and Metabolic Syndrome: Evidence from Human and Model System Studies

Evaluation of the Effects of Fluoride and Associated With Lead in Animal Model and Physical-Chemical Analysis of Public Supply Water and of the Sinos River in the South of Brazil

Contact Info

Product

Resources

About