Lead Exposure Assessment and Its Impact on the Structural Organization and Morphological Peculiarities of Rat Ovaries

Osowski, Adam; Fedoniuk, Larysa; Bilyk, Yaroslav; Fedchyshyn, Olena; Sas, Mykhailo; Kramar, Solomiia; Lomakina, Yuliia; Fik, Volodymyr; Chorniy, Sofija; Wojtkiewicz, Joanna

doi:10.3390/toxics11090769

Cited by 1 publication

(2 citation statements)

References 45 publications

(105 reference statements)

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“…Treatment with ASFE following lead acetate intoxication led to a significant improvement in the % b. wt of rats. These findings agreed with previous investigations reporting weight loss and reduced intestinal absorption upon exposure to lead in experimental animals [51]. A previous study reported the improvement of body weight loss in rats that was induced by lead upon treatment with natural products.…”

Section: Discussionsupporting

confidence: 93%

“…Adeyi et al (2023) investigated the beneficial role of ferulic acid as an anti-inflammatory agent via the modulation of SOCS-1 signaling pathways in testicular inflammation in a rat model [66]. Lead acetate exposure caused significant histopathological alterations in the testis architectures that were consistent with previous studies [51,56,72]. However, in this investigation, treatment with ASFE for lead acetate intoxication improved sperm characteristics significantly and affirmed the normal histological structure of most seminiferous tubules.…”

Section: Discussionsupporting

confidence: 85%

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Annona squamosa Fruit Extract Ameliorates Lead Acetate-Induced Testicular Injury by Modulating JAK-1/STAT-3/SOCS-1 Signaling in Male Rats

Mobasher,

Alsirhani,

Alwaili

et al. 2024

IJMS

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Lead (Pb) is a common pollutant that is not biodegradable and gravely endangers the environment and human health. Annona squamosa fruit has a wide range of medicinal uses owing to its phytochemical constituents. This study evaluated the effect of treatment with A. squamosa fruit extract (ASFE) on testicular toxicity induced in male rats by lead acetate. The metal-chelating capacity and phytochemical composition of ASFE were determined. The LD50 of ASFE was evaluated by probit analysis. Molecular docking simulations were performed using Auto Dock Vina. Forty male Sprague Dawley rats were equally divided into the following groups: Gp1, a negative control group; Gp2, given ASFE (350 mg/kg body weight (b. wt.)) (1/10 of LD50); Gp3, given lead acetate (PbAc) solution (100 mg/kg b. wt.); and Gp4, given PbAc as in Gp3 and ASFE as in Gp2. All treatments were given by oro-gastric intubation daily for 30 days. Body weight changes, spermatological parameters, reproductive hormone levels, oxidative stress parameters, and inflammatory biomarkers were evaluated, and molecular and histopathological investigations were performed. The results showed that ASFE had promising metal-chelating activity and phytochemical composition. The LD50 of ASFE was 3500 mg/kg b. wt. The docking analysis showed that quercetin demonstrated a high binding affinity for JAK-1 and STAT-3 proteins, and this could make it a more promising candidate for targeting the JAK-1/STAT-3 pathway than others. The rats given lead acetate had defective testicular tissues, with altered molecular, biochemical, and histological features, as well as impaired spermatological characteristics. Treatment with ASFE led to a significant mitigation of these dysfunctions and modulated the JAK-1/STAT-3/SOCS-1 axis in the rats.

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Section: Discussionsupporting

confidence: 93%