Lead contamination among Paris Fire Brigade firefighters who fought the Notre Dame Cathedral fire in Paris

Allonneau, Alexandre; Mercier, Samuel; Maurin, Olga; Robardet, Fabien; Menguy-Fleuriot, A.; Luu, Say-Chong; Louyot, Catherine; Jacques, Nicole.D; Jouffroy, Romain; Prunet, Bertrand

doi:10.1016/j.ijheh.2021.113707

Cited by 7 publications

(1 citation statement)

References 2 publications

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“…The neurological effects of high Pb exposure include delusion, impaired judgment, and even a desire to commit murder. An increased Pb content in honey produced near the Cathedral of Notre-Dame de Paris in France after a 2019 fire was found to derive from old leaded stained glass that was destroyed by the fire, producing fumes that were spread by the wind [7][8][9]. More recently, a doctor of Chinese medicine in Taiwan illegally issued an incorrect prescription containing large amounts of Pb, which led to harmful effects for patients with ages ranging from 4 to 85 years old.…”

Section: Introductionmentioning

confidence: 99%

Versatile Cell and Animal Models for Advanced Investigation of Lead Poisoning

Yang

Chang²

2021

Preprint

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The heavy metal lead (Pb) can irreversibly damage the human nervous system. To help understand Pb-induced damage, we have developed practical applications for genetically encoded Pb biosensors in cardiac cells and insect central nervous tissue. We applied the optimized fluorescence resonance energy transfer (FRET)-based Pb biosensor Met-lead 1.44 M1 to two living systems to monitor the concentration of Pb: induced pluripotent stem cell (iPSC)-derived cardiomyocytes as a semi-tissue platform, and Drosophila melanogaster fruit flies as an in vivo animal model. Different FRET imaging modalities were used to obtain FRET signals, which repre-sented the presence of Pb in the tested samples in different spatial dimensions. Pb was effectively sensed in two living models producing Met-led 1.44 M1. In iPSC-derived cardiomyocytes, the relationship between beating rate determined from the fluctuation of fluorescent signals and the concentrations of Pb represented by the FRET emission ratio values of Met-lead 1.44 M1 demonstrated the potential of this fluorescence biosensor system for anti-Pb drug screening. In the Drosophila model, Pb was detected within the adult brain or larval central nervous system using fast epifluorescence and high-resolution two-photon 3D FRET ratio image sys-tems. The optimized Pb biosensor together with FRET microscopy can be used for specific applications to de-tect Pb with a limit of detection of 10 nM (2 ppb). We believe that this integrated Pb biosensor system can be applied to the prevention of Pb poisoning.

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Section: Introductionmentioning

confidence: 99%