Lead concentration and the level of glutathione, glutathione S-transferase, reductase and peroxidase in the blood of some occupational workers from Irbid City, Jordan

Hunaiti, Abdelrahim A.; Soud, M.; Khalil, Ashraf A.

doi:10.1016/0048-9697(95)04606-2

Cited by 38 publications

(23 citation statements)

References 10 publications

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“…GR, the enzyme responsible for recycling of glutathione from the oxidized form (glutathione disulfide; GSSG) to the reduced form (reduced glutathione; GSH) is also deactivated by lead (Hunaiti et al, 1995). Depressed levels of glutathione reductase, glutathione peroxidase, and glutathione-S-transferase were all found to correlate with depressed glutathione levels in occupationally-exposed workers (Sandhir and Gill, 1995).…”

Section: Discussionmentioning

confidence: 84%

Role Of Wormwood (<i>Artemisia absinthium</i>) Extract On Oxidative Stress In Ameliorating Lead Induced Haematotoxicity.

Kharoubi¹,

Slimani²,

Krouf³

et al. 2008

Afr. J. Trad. Compl. Alt. Med.

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Effects of ROS generation have been postulated to be major contributors to lead-exposure related disease. The aim of the study was to investigate the effect of aqueous extract of wormwood (Artemisia absinthium) on oxidative stress in rats protractedly exposed to lead. Aqueous extract of wormwood plant was administered orally (200 mg.kg -1 body weight). Plasma vitamin C, E and non-protein thiol concentrations, red blood cells (RBC) thiobarbituric acid reactive substances, reduced glutathione levels and haemolysis test were evaluated. In addition, RBC antioxidant enzymes activities such as superoxide dismutases, catalase, glutathione peroxidase, glutathione reductase were also estimated. After 11-weeks, significant decreases of plasma vitamin C, E, non protein-thiol (NP-SH) and RBC-reduced glutathione levels were observed in Pb compared to control group (-32.9%, -57.1%, -53.1%, -33.9%, respectively); superoxide dismutase, glutathione peroxidase, uric aminolevulinic acid and haemolysis test significantly increased in Pb compared to control group (+64.3%, +40.3%, +145%, +44.3%, respectively). In our investigation, after 4-weeks of treatment all treated groups did not show any difference compared to the control group, except for glutathione peroxidase and RBC-superoxide dismutase activity (-15.7% and +16.4%, respectively). The findings of this study suggest that wormwood (Artemisia absinthium) extract restored the enzymes activities perturbed by exposure to lead, and had a protective role against lipid peroxidation

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Section: Discussionmentioning

confidence: 84%

Role Of Wormwood (<i>Artemisia absinthium</i>) Extract On Oxidative Stress In Ameliorating Lead Induced Haematotoxicity.

Kharoubi¹,

Slimani²,

Krouf³

et al. 2008

Afr. J. Trad. Compl. Alt. Med.

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“…The enzyme is particularly important under limited glutathione content conditions, such as obtain in lead (Pb)-exposed animals (Hsu, 1981;Hunaiti et al, 1995), and plays a significant role in the development of tolerance to oxidative stress in the adaptive responses of cells (Wassmann et al, 2004 (Grosch et al, 1998;Silber et al, 2002). Conversely, overexpression of Ref-1 repair functions stimulates an increase in resistance to certain alkylating agents and oxidative stresses (Tomicic et al, 1997;Grosch et al, 1998;Kim et al, 2006;Lee et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

Overexpression of Ref-1 Inhibits Lead-induced Endothelial Cell Death via the Upregulation of Catalase

Lee

Kim

et al. 2009

Korean J Physiol Pharmacol

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“…The binding of lead to glutathione has been investigated, however little information is available regarding the detailed molecular nature of the binding in lymphocytes. Concentrations of glutathione in the blood have been reported to be significantly lower than control levels both in animal studies of lead exposure and in lead-exposed children and adults (Hsu, 1981;Hunaiti et al, 1995). It is believed that lead interacts with glutathione that exists in lymphocytes and thus forms lead-GSH conjugates which might be the mechanisms of uptake inhibition and decreased toxicity of lead in the cells.…”

Section: Discussionmentioning

confidence: 99%

A change in metabolic status of glutathione (-L-glutamyl-2-cysteinyl-glycine) in lymphocytes by lead compounds

Ullah¹

2013

Afr. J. Pharm. Pharmacol.

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A spectrophotometric investigation of the interaction and coordination of lead compounds with glutathione (GSH) in lymphocytes of healthy male volunteer venous blood has been described. The present study was designed to provide insight into the proposed mechanism of action of lead acetate {Pb(CH 3 COO) 2 } and lead acetylacetonate (C 10 H 14 O 4 Pb) with GSH at molecular level. Lymphocytes were separated from blood of healthy human volunteers. Different concentrations of lead acetate and lead acetylacetonate were used and their concentration, time, pH and temperature dependent effects on GSH level was studied. Ellman's method was used for the determination of GSH. With lead compounds, a decreased concentration of GSH was found, which further decreased with increasing concentration of lead compounds. A more decrease in GSH concentration was found with time-dependent (0-90 minutes) incubation of lymphocytes with lead compounds. pH, 8.5 and temperature, 45°C have been found to be most favorable for reactions of lead compound with GSH. It may be suggested that changes in GSH status produced by lead compounds could be due to the formation of conjugates (Pb-SG) between GSH and lead compounds or conversion of GSH to oxidized GSH (GSSG) in lymphocytes. The interaction and coordination of lead compounds with sulphur suggest the sulfhydryl (-SH) group to be the coordinating site of GSH.

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Lead concentration and the level of glutathione, glutathione S-transferase, reductase and peroxidase in the blood of some occupational workers from Irbid City, Jordan

Cited by 38 publications

References 10 publications

Role Of Wormwood (<i>Artemisia absinthium</i>) Extract On Oxidative Stress In Ameliorating Lead Induced Haematotoxicity.

Role Of Wormwood (<i>Artemisia absinthium</i>) Extract On Oxidative Stress In Ameliorating Lead Induced Haematotoxicity.

Overexpression of Ref-1 Inhibits Lead-induced Endothelial Cell Death via the Upregulation of Catalase

A change in metabolic status of glutathione (-L-glutamyl-2-cysteinyl-glycine) in lymphocytes by lead compounds

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