Leachate from microplastics impairs larval development in brown mussels

Silva, Pablo Pena Gandara e; Nobre, Caio Rodrigues; Resaffe, Pryscila; Pereira, Camilo Dias Seabra; Gusmão, Felipe

doi:10.1016/j.watres.2016.10.016

Cited by 248 publications

(82 citation statements)

References 45 publications

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“…This hypothesis is consistent with the observed more pronounced effects of leachates from beached than from virgin pellets. The relatively high (though locally realistic) pellet concentration considered here and the lack of information on the actual pollutants at play stress the need to assess L. littorea behavioural traits in response to various leachates concentrations and specific pollutants that are both likely to be seasonal and site-dependent [12]. Note, however, that a 24-h incubation time is extremely short compared to the residence times of microplastic pellets in the ocean [1,2], which may indicate that the pollutant concentrations assessed here are not irreconcilable with in situ concentrations.…”

Section: Resultsmentioning

confidence: 99%

“…Microplastic leachate seawater was prepared from commercially available virgin polypropylene pellets (SW VP ), and from beached pellets (SW BP ) haphazardly collected from the high-tide mark sediment surface of beaches surrounding the reef. Pellets (diameter 3.3-4.7 mm) were mixed with seawater at a concentration of 20 ml of pellets per litre-a concentration one order of magnitude lower than those that impaired the embryonic development of mussels and sea urchins [12,13], which is not uncommon in the sampling area-and left for 24 h before the beginning of the behavioural assays. Crab cue seawater (SW CC ) was prepared through the addition of an individual C. maenas (carapace width 80.3 + 3.3 mm) into 5 l of aerated seawater for a 48 h period prior to the trial.…”

Section: Methodsmentioning

confidence: 99%

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Microplastic leachates impair behavioural vigilance and predator avoidance in a temperate intertidal gastropod

Seuront

2018

Biol. Lett.

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Microplastics are a ubiquitous source of contaminations in marine ecosystems, and have major implications for marine life. Much effort has been devoted to assessing the various effects of microplastics on marine life. No evidence exists, however, on the effects of microplastic leachates on chemically mediated predator-prey interactions and the ability of prey to detect and avoid its predator. This study shows that microplastic leachates have direct biological effects by disturbing the behavioural response of the intertidal gastropod Littorina littorea to the presence of Carcinus maenas chemical cues, hence increasing their vulnerability to predation. Leachates from virgin and beached pellets respectively impaired and inhibited L. littorea vigilance and antipredator behaviours. These results suggest that the biological effects from microplastic leachates may have major implications for marine ecosystems on taxa that rely on chemosensory cues to escape predation.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Microplastic leachates impair behavioural vigilance and predator avoidance in a temperate intertidal gastropod

Seuront

2018

Biol. Lett.

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“…Histopathological results revealed changes in gut mucosa and hemocyte infiltration in conjunctive tissue of digestive gland after seven days of exposure. After exposed to polypropylene (PP) microplastic leachate, the brown mussels showed embryo‐toxicity and delayed larval growth . When studied on a molecular level, MPs prefer to interfere with the synthesis of rhamnose and xylose of exopolysaccharide biosynthesis pathway .…”

Section: Discussionmentioning

confidence: 99%

“…After exposed to polypropylene (PP) microplastic leachate, the brown mussels showed embryotoxicity and delayed larval growth. 48 When studied on a molecular level, MPs prefer to interfere with the synthesis of rhamnose and xylose of exopolysaccharide biosynthesis pathway. 49 In invertebrates, MPs destroyed the filtration procedure which resulted in the reduction of food consumption and casualty in crustaceans.…”

Section: Validation By Quantitative Rt-qpcr and Elisamentioning

confidence: 99%

Effects of polystyrene microbeads on cytotoxicity and transcriptomic profiles in human Caco‐2 cells

Tian

et al. 2019

Environmental Toxicology

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Microplastics (MPs) pollution is a global paradigm that raises concern in relation to environment and human health. In order to investigate the molecular toxicity mechanisms of MPs, transcriptomic analyses were performed on in vitro Caco‐2 cell model. After observing that polystyrene microplastics (PS‐MPs) decreased cell viability in a dose‐dependent manner, the responsible genes and involved pathways that might make contribution to PS‐MBs‐induced toxicity to Caco‐2 cells were identified with Illumina RNA seq. A total of 442 genes including, 210 up‐regulated ones and 232 down‐regulated ones, showed differential expression after treatment by PS‐MPs with a concentration of 12.5 mg L−1 or 50.0 mg L−1 for 24 hours. Gene Ontology (GO) annotation enriched unigenes can be grouped into three separated clusters: cellular component (CC), biological process (BP), and molecular function (MF). The dominate pathways related to NF‐κB, MAPK signaling, cytokine‐cytokine receptor interaction, and toll‐like receptor were strongly influenced by PS‐MBs. These pathways are involved in modulating cell inflammatory and proliferation. The qPCR were applied to investigate the transcriptional level of five proliferation related genes (Ras, ERK, MER, CDK4, Cyclin D1) and four inflammation related genes (TRPV1, iNOS, IL‐1β, IL‐8), and the results were consistent with RNA‐seq data. This study has provided new insight into the understanding of the toxicity effects of PS‐MBs‐induced intestinal inflammatory diseases.

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“…Very scant data are available regarding the effects of MPs on the development of aquatic organisms, as most studies have focused on the chemical aspects of MPs. In fact, the toxic effects of leachate from particles have been analyzed in different organisms, such as mussels (Gandara e Silva et al, 2016), fish (Lonnstedt and Eklov, 2016) and sea urchins (Nobre et al, 2015), whereas the physical effects are still scarcely explored (Kaposi et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Effects of polystyrene microplastics on early stages of two marine invertebrates with different feeding strategies

Messinetti

Mercurio

Parolini

et al. 2018

Environmental Pollution

141

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Nowadays, microplastics represent one of the main threats to marine ecosystems, being able to affect organisms at different stages of their life cycle and at different levels of the food web. Although the presence of plastic debris has been reported in different habitats and the ability to ingest it has been confirmed for different taxa, few studies have been performed to elucidate the effects on survival and development of marine animals. Thus, we explored the effects of different environmental concentrations of polystyrene microbeads on the early stages of two invertebrate species widespread in the Mediterranean shallow waters: the pelagic planktotrophic pluteus larvae of the sea urchin Paracentrotus lividus and the filter-feeding sessile juveniles of the ascidian Ciona robusta. We evaluated the effects on larvae and juvenile development and determined the efficiency of bead ingestion. The feeding stages of both species proved to be extremely efficient in ingesting microplastics. In the presence of microbeads, the metamorphosis of ascidian juveniles was slowed down and development of plutei altered. These results prompted the necessity to monitor the populations of coastal invertebrates since microplastics affect sensitive stages of life cycle and may have consequences on generation recruitment.

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Leachate from microplastics impairs larval development in brown mussels

Cited by 248 publications

References 45 publications

Microplastic leachates impair behavioural vigilance and predator avoidance in a temperate intertidal gastropod

Microplastic leachates impair behavioural vigilance and predator avoidance in a temperate intertidal gastropod

Effects of polystyrene microbeads on cytotoxicity and transcriptomic profiles in human Caco‐2 cells

Effects of polystyrene microplastics on early stages of two marine invertebrates with different feeding strategies

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