LEA Gene Introns: is the Intron of Dehydrin Genes a Characteristic of the Serine-Segment?

Jiménez-Bremont, Juan Francisco; Maruri-López, Israel; Ochoa-Alfaro, Ana Erika; Delgado‐Sánchez, Pablo; Bravo, Jaime; Rodríguez‐Kessler, Margarita

doi:10.1007/s11105-012-0483-x

Cited by 15 publications

(12 citation statements)

References 39 publications

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“…All the introns identified are located in the middle of the Ssegment as previously observed in dehydrins isolated not only from P. pinaster but also from other plants (Jiménez-Bremont et al 2013;Perdiguero et al 2012a). Seven sequences showing a complete deduced amino acid sequence were aligned, and its composition in conserved segments analysed (Fig.…”

Section: Resultssupporting

confidence: 70%

Comparative analysis of Pinus pinea and Pinus pinaster dehydrins under drought stress

Perdiguero

Soto

Collada

2015

Tree Genetics & Genomes

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Maritime pine (Pinus pinaster) and stone pine (P. pinea) are two of the most drought-resistant Mediterranean trees. Several studies have analysed the response to drought stress at the molecular level in maritime pine, including the identification of drought-induced genes, transcriptomic analysis during dehydration or in-depth characterisation and diversity studies of specific candidate genes. On the contrary, much less information is available for stone pine, and notwithstanding being a closely related species, significant differences in the transcription profile of several genes during drought, evaluated using microarrays, were reported recently for these two species. In this study, we focus on P. pinea dehydrins, one of the most important gene families expressed in response to drought. We have identified eight dehydrin genes in P. pinea, orthologous to the ones previously described in P. pinaster, and have compared their transcription profiles under drought stress. For this purpose, we imposed a severe and prolonged drought treatment to P. pinea seedlings and analysed the expression pattern of proteins from the dehydrin family in needles, stems and roots. The complete open reading frames of these genes were amplified from cDNA and genomic DNA, and their intron/exon structures were determined. qRT-PCR was performed to analyse their expression pattern in needles, stems and roots during the drought treatment. Remarkable differences between the two species have been detected in the transcript patterns of five out of the eight genes, which could be related with the different behaviours described for these species under drought stress.

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Section: Resultssupporting

confidence: 70%

Comparative analysis of Pinus pinea and Pinus pinaster dehydrins under drought stress

Perdiguero

Soto

Collada

2015

Tree Genetics & Genomes

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“…They also accumulate discrepancies in the A-segment and present extremely short S-segments, with 4–5 serine residues, while other P. pinaster dehydrins have 7–9 residues. The three genes include an intron within the S-segment, a feature also found in other P. pinaster dehydrins (Perdiguero et al, 2012a ), as well as in SKn-type dehydrins from angiosperms (Jiménez-Bremont et al, 2013 ).…”

Section: Discussionmentioning

confidence: 83%

Novel dehydrins lacking complete K-segments in Pinaceae. The exception rather than the rule

2014

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Dehydrins are thought to play an essential role in the plant response, acclimation and tolerance to different abiotic stresses, such as cold and drought. These proteins contain conserved and repeated segments in their amino acid sequence, used for their classification. Thus, dehydrins from angiosperms present different repetitions of the segments Y, S, and K, while gymnosperm dehydrins show A, E, S, and K segments. The only fragment present in all the dehydrins described to date is the K-segment. Different works suggest the K-segment is involved in key protective functions during dehydration stress, mainly stabilizing membranes. In this work, we describe for the first time two Pinus pinaster proteins with truncated K-segments and a third one completely lacking K-segments, but whose sequence homology leads us to consider them still as dehydrins. qRT-PCR expression analysis show a significant induction of these dehydrins during a severe and prolonged drought stress. By in silico analysis we confirmed the presence of these dehydrins in other Pinaceae species, breaking the convention regarding the compulsory presence of K-segments in these proteins. The way of action of these unusual dehydrins remains unrevealed.

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“…We have previously reported on an O. streptacantha dehydrin gene, encoding an SK 3 -type acidic protein (Ochoa-Alfaro et al, 2012 ; Jiménez-Bremont et al, 2013 ). This OpsDHN1 protein contains three K-segments (lysine-rich repeat) at the central (residues 98–112) and C-terminal regions (residues 172–186 and 212–226), and a serine repeat segment (S-segment) located at residues 75–79.…”

Section: Resultsmentioning

confidence: 99%

“…We previously reported that the O. streptacantha SK 3 DHN (OpsDHN1) is induced by cold, and plays a role in plant freezing stress tolerance (Ochoa-Alfaro et al, 2012 ; Jiménez-Bremont et al, 2013 ). In this study, we demonstrate that OpsDHN1 interacts with itself using a split-ubiquitin yeast two-hybrid system (Figure 2 ).…”

Section: Discussionmentioning

confidence: 99%

A dehydrin-dehydrin interaction: the case of SK3 from Opuntia streptacantha

et al. 2014

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Dehydrins belongs to a large group of highly hydrophilic proteins known as Late Embryogenesis Abundant (LEA) proteins. It is well known that dehydrins are intrinsically disordered plant proteins that accumulate during the late stages of embryogenesis and in response to abiotic stresses; however, the molecular mechanisms by which their functions are carried out are still unclear. We have previously reported that transgenic Arabidopsis plants overexpressing an Opuntia streptacantha SK3 dehydrin (OpsDHN1) show enhanced tolerance to freezing stress. Herein, we show using a split-ubiquitin yeast two-hybrid system that OpsDHN1 dimerizes. We found that the deletion of regions containing K-segments and the histidine-rich region in the OpsDHN1 protein affects dimer formation. Not surprisingly, in silico protein sequence analysis suggests that OpsDHN1 is an intrinsically disordered protein, an observation that was confirmed by circular dichroism and gel filtration of the recombinantly expressed protein. The addition of zinc triggered the association of recombinantly expressed OpsDHN1 protein, likely through its histidine-rich motif. These data brings new insights about the molecular mechanism of the OpsDHN1 SK3-dehydrin.

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LEA Gene Introns: is the Intron of Dehydrin Genes a Characteristic of the Serine-Segment?

Cited by 15 publications

References 39 publications

Comparative analysis of Pinus pinea and Pinus pinaster dehydrins under drought stress

Comparative analysis of Pinus pinea and Pinus pinaster dehydrins under drought stress

Novel dehydrins lacking complete K-segments in Pinaceae. The exception rather than the rule

A dehydrin-dehydrin interaction: the case of SK3 from Opuntia streptacantha

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