Le renouveau de l’interleukine 2

Jacques, Yannick; Mortier, Erwan

doi:10.1051/medsci/20163206025

Cited by 1 publication

(1 citation statement)

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“…Interestingly in the present study, high frequencies of total CD3+ and CD4+ T cells were concomitant with high serum IL-2 and IFN-γ concentrations observed in insulintreated pregnant women with T2D and women with GDM compared to pregnant controls. In fact, being a T cell growth factor, IL-2 could induce the increase of T cells by its autocrine action [52]. High production of IFN-γ and low level of IL-4 confirmed the proinflammatory Th1 phenotype in insulin-treated T2D pregnant women as well as in women with GDM in this study.…”

Section: Discussionsupporting

confidence: 77%

Th2-Immune Polarizing and Anti-Inflammatory Properties of Insulin Are Not Effective in Type 2 Diabetic Pregnancy

Fagninou

Nekoua

Sossou³

et al. 2020

Journal of Immunology Research

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Background. The implication of the immune system in the physiopathology of pregnancy complicated by diabetes has been reported. Here, we investigated the effects of insulin treatment on the frequencies of immune cell subpopulations as well as T cell-derived cytokines in type 2 diabetic (T2D) pregnancy compared to gestational diabetes mellitus (GDM). Methods. Fifteen (15) women with GDM, twenty (20) insulin-treated T2D pregnant women, and twenty-five (25) pregnant controls were selected. Immune cell subpopulation frequencies were determined in blood using flow cytometry. The proliferative capacity of T cells was performed, and serum and cell culture supernatant cytokine levels were also quantified. Results. The frequencies of total CD3+ and CD4+ T cells and nonclassical monocytes significantly increased in insulin-treated T2D pregnant women compared to pregnant controls. The proportions of CD4+ T cells as well as B cells were significantly higher in women with GDM than in pregnant controls. GDM was associated with high frequencies of total CD3+ and CD4+ T cells and B cell expansion, suggesting a concomitant activation of cellular and humoral immunity. Concomitantly, Th1/Th2 ratio, determined as IFN-γ/IL-4, was shifted towards Th1 phenotype in women with GDM and insulin-treated T2D pregnant women. Besides, isolated T cells elicited similar proliferative capacity in the three groups of women. Insulin-treated T2D pregnant women and women with GDM exhibited a low serum IL-10 level, without any change in the number of Treg cells. Conclusion. Our study showed that, despite insulin treatment, pregnant women with T2D displayed a proinflammatory status consistent with high proportions of CD3+ and CD4+ T cells, upregulation of Th1 cytokines, and low IL-10 production, suggesting a reduced immune-suppressive activity of regulatory T cells. However, GDM, although associated with proinflammatory status, has shown increased humoral immunity consistent with high proportion of CD19+ B cells. Thus, the lack of response to insulin in diabetes during pregnancy and clinical implications of these immunological parameters deserves further investigations.

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Section: Discussionsupporting

confidence: 77%