Abstract Baratte, S., Cobb, M., Deutsch, J. and Peeters, C. 2005. Morphological variations in the pre-imaginal development of the ponerine ant Diacamma ceylonense . -Acta Zoologica (Stockholm) 86 : 25-31In the present paper, the larval development of the queenless ponerine ant Diacamma ceylonense is studied. Four instars were identified on the basis of cuticular processes -tubercles and spinules -which show discontinuous variation during growth and provide precise and reliable external morphological criteria for instar discrimination. In the first three instars, the larva possesses a striking proboscis, which disappears between the third and fourth instars. Larval weight increased around 50-fold in the 21 days of larval life between eclosion and pupation (mean weight for first instar = 0.37 mg; for fourth instar = 20 mg). In addition, the morphology of the gemmae, structures resembling vestigial wings in workers, is described in nymphae.