Le point sur les transporteurs d'oxygène à base d 'hémoglobine

Pagnier, J.; Marden, Michael C.; Poyart, C

doi:10.4267/10608/676

Cited by 5 publications

(2 citation statements)

References 2 publications

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“…The oxygen affinity is perhaps the parameter that has seen the most progress (Fig. 1); various mutations and crosslinks have provided a wide range of oxygen affinities [3].…”

Section: Resultsmentioning

confidence: 99%

“…Other oxidation reactions or bacterial growth may also be enhanced due to the iron and hemin available from the degrading Hb solution [1,2]. While much progress has been made in regulating the oxygen affinity and stabilizing the tetrameric form, the problems of oxidation of the Hb and related reactions due to the degradation of the Hb remain [3].…”

Section: Introductionmentioning

confidence: 99%

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Modulation of the oxygen affinity of cobalt‐porphyrin by globin

et al. 2000

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We have combined two extreme effects which influence the oxygen affinity to obtain a cobalt-based oxygen carrier with an affinity similar to that of human adult hemoglobin (HbA). The goal was to obtain an oxygen transporter with a lower oxidation rate. Exchange of the heme group (Feprotoporphyrin IX) in Hb with a cobalt-porphyrin leads to a reduction in oxygen affinity by over a factor of 10, an oxygen affinity too low for use as a blood substitute. At the other extreme, certain globin sequences are known to provide a very high oxygen affinity; for example, Hb Ascaris displays an oxygen affinity 1000 times higher than HbA. We demonstrate here that these opposing effects can be additive, yielding an oxygen affinity similar to that of HbA, but with oxygen binding to a cobalt atom. We have tested the effect of substitution of cobalt-porphyrin for heme in normal HbA, sperm whale (SW) Mb (Mb), and high affinity globins for leghemoglobin, two trematode Hbs: Paramphistomum epiclitum (Pe) and Gastrothylax crumenifer (Gc). As for HbA or SW Mb, the transition from heme to cobalt-porphyrin in the trematode Hbs leads to a large decrease in the oxygen affinity, with oxygen partial pressures for half saturation (P 50 ) of 5 and 25 mm Hg at 37³C for cobalt-Pe and cobalt-Gc, respectively. A critical parameter for Hb-based blood substitutes is the autoxidation rate; while both metals oxidize to an inactive state, we observed a decrease in the oxidation rate of over an order of magnitude for cobalt versus iron, for similar oxygen affinities. The time constants for autoxidation at 37³C were 250 and 100 h for Pe and Gc, respectively.z 2000 Federation of European Biochemical Societies.

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“…The oxygen affinity is perhaps the parameter that has seen the most progress (Fig. 1); various mutations and crosslinks have provided a wide range of oxygen affinities [3].…”

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Modulation of the oxygen affinity of cobalt‐porphyrin by globin

et al. 2000

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Transfusion Medicine

Lacroix¹,

Tucci²,

Tinmouth³

et al. 2011

Pediatric Critical Care

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Novel foods and food ingredients: what is the mission of scientists and technologists?

Мота

Empis²

2000

Trends in Food Science & Technology

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The situation concerning world food production is changing dramatically. On the one hand, the world population is expected to grow still further, reaching about 8 billion people in the middle of the 21st century. Novel phenomena, such as ageing of the human population and excess weight in developing countries pose new nutrition problems. Food scares, which were enhanced by recent BSE and toxicological outbreaks, hinder the applicability of some solutions. Under these conditions, scientists and technologists should participate actively in the tasks of informing the population as well as ®nding new ways to provide people with the optimal amount of nutritious food.

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Le point sur les transporteurs d'oxygène à base d 'hémoglobine

Cited by 5 publications

References 2 publications

Modulation of the oxygen affinity of cobalt‐porphyrin by globin

Modulation of the oxygen affinity of cobalt‐porphyrin by globin

Transfusion Medicine

Novel foods and food ingredients: what is the mission of scientists and technologists?

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