LDL receptor and its family members serve as the cellular receptors for vesicular stomatitis virus

Finkelshtein, Danit; Werman, Ariel; Novick, Daniela; Barak, Sara; Rubinstein, Menachem

doi:10.1073/pnas.1214441110

Cited by 486 publications

(433 citation statements)

References 52 publications

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“…8 Indeed, in resting lymphocytes, LV postentry steps such as completion of reverse transcription, nuclear import, and chromosomal integration of the transgene do not readily occur. [8][9][10] Moreover, we confirmed low expression levels of the VSV receptor (ie, low-density lipoprotein receptor 11 ) at the surface of unstimulated CD34…”

Section: Introductionsupporting

confidence: 51%

Measles virus envelope pseudotyped lentiviral vectors transduce quiescent human HSCs at an efficiency without precedent

et al. 2017

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Section: Introductionsupporting

confidence: 51%

Measles virus envelope pseudotyped lentiviral vectors transduce quiescent human HSCs at an efficiency without precedent

et al. 2017

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“…The enhanced effect in VSV-G mediated transduction could possibly be due to similar mechanism of viral entry mediated by GP64 and VSV-G as suggested previously [12]. It is also likely that widespread expression of LDL receptor and its family members that serve as major cell entry port for VSV [7], may provide a positive effect in VSV-G pseudotyped viral vectors. The observed variation in VSV-G mediated transduction efficiency in different cell types could be due to difference in receptor(s) expression.…”

Section: Discussionsupporting

confidence: 57%

Baculovirus mediated transduction: analysis of vesicular stomatitis virus glycoprotein pseudotyping

et al. 2014

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The recombinant baculoviruses were constructed to investigate the necessity of VSV-G pseudotyping for mammalian cell transduction. The viruses were designed to express green fluorescent protein (GFP) gene under the control of cytomegalovirus promoter, with or without pseudotyping with VSV-G. VSV-G was placed under the control of polyhedrin promoter that is recognized by insect cells, allowing the formation of pseudotyped baculovirus. The study findings demonstrate that the pseudotyping of baculovirus significantly enhanced transduction efficiency compared to non-pseudotyped baculovirus, resulting in consequent distinction in the expression of GFP in mammalian cells. The results confirmed that pseudotyping is important for baculovirus mediated gene delivery. Further, when full-length VSV-G pseudotyping was compared with truncated VSV-G containing GED domain (G-stem of ectodomain in conjunction with the TM and CT domains of the glycoprotein), latter was relatively less efficient in transducing mammalian cells. This study demonstrated that pseudotyping with full-length VSV-G had better transduction efficiency in mammalian cells. However, at higher multiplicity of infection, both fulllength and truncated VSV-G showed equivalent transduction. This study established the significance of pseudotyping of baculovirus with full-length VSV-G for efficient transduction of mammalian cells, utilizing the highly sensitive GFP marker system. These findings have significant implications in designing of baculovirus vector based antigen delivery for developing new generation vaccines.

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“…To further investigate this question, we performed an additional antibody-blocking study. It was recently shown that the primary VSV receptor on mammalian cells belongs to the LDL receptor family and that VSV entry can be inhibited by preincubating target cells with an anti-LDLR antibody (45). We therefore tested the infectivities of VSV, Maraba virus, and VSV/Maraba G on K562-av␤3 cells that had been pretreated with this VSV receptor-blocking antibody or control measles virus receptor binding antibody (anti-CD46 antibody).…”

Section: Resultsmentioning

confidence: 99%

Vesiculovirus Neutralization by Natural IgM and Complement

Tesfay

Ammayappan

Federspiel

et al. 2014

J Virol

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Because of its very low human seroprevalence, vesicular stomatitis virus (VSV) has promise as a systemic oncolytic agent for human cancer therapy. However, as demonstrated in this report, the VSV infectious titer drops by 4 log units during the first hour of exposure to nonimmune human serum. This neutralization occurs relatively slowly and is mediated by the concerted actions of natural IgM and complement. Maraba virus, whose G protein is about 80% homologous to that of VSV, is relatively resistant to the neutralizing activity of nonimmune human serum. We therefore constructed and rescued a recombinant VSV whose G gene was replaced by the corresponding gene from Maraba virus. Comparison of the parental VSV and VSV with Maraba G substituted revealed nearly identical host range properties and replication kinetics on a panel of tumor cell lines. Moreover, in contrast to the parental VSV, the VSV with Maraba G substituted was resistant to nonimmune human serum. Overall, our data suggest that VSV with Maraba G substituted should be further investigated as a candidate for human systemic oncolytic virotherapy applications. IMPORTANCEOncolytic virotherapy is a promising approach for the treatment of disseminated cancers, but antibody neutralization of circulating oncolytic virus particles remains a formidable barrier. In this work, we developed a pseudotyped vesicular stomatitis virus (VSV) with a glycoprotein of Maraba virus, a closely related but serologically distinct member of the family Rhabdoviridae, which demonstrated greatly diminished susceptibility to both nonimmune and VSV-immune serum neutralization. VSV with Maraba G substituted or lentiviral vectors should therefore be further investigated as candidates for human systemic oncolytic virotherapy and gene therapy applications.

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LDL receptor and its family members serve as the cellular receptors for vesicular stomatitis virus

Cited by 486 publications

References 52 publications

Measles virus envelope pseudotyped lentiviral vectors transduce quiescent human HSCs at an efficiency without precedent

Measles virus envelope pseudotyped lentiviral vectors transduce quiescent human HSCs at an efficiency without precedent

Baculovirus mediated transduction: analysis of vesicular stomatitis virus glycoprotein pseudotyping

Vesiculovirus Neutralization by Natural IgM and Complement

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