LDL-induced association of anionic liposomes with cells and delivery of contents

Amin, Ketan; Heath, Timothy D.

doi:10.1016/s0168-3659(01)00278-4

Cited by 10 publications

(13 citation statements)

References 23 publications

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“…Previous studies have shown that anionic liposomes (containing at least 75% PG) can interact with LDL particles, either through direct coalescence (17,18) or an electrostatic interaction between negatively-charged phospholipids and apoB-100 as a highly cationic protein (34,35).…”

Section: Cytotoxicity Assaysmentioning

confidence: 99%

“…Removal of resulting liposome-LDL complexes would be mediated by LDL receptor or macrophages (17)(18)(19). This simple mechanism enhances the delivery of serum cholesterol to the hepatic tissue where cholesterol can be excreted ion.…”

Section: Cytotoxicity Assaysmentioning

confidence: 99%

“…There is evidence indicating that liposomes containing at least 75% anionic phospholipids in their composition can coalesce with LDL particles, resulting in the formation of particles that are cleared through either hepatocyte LDL receptors or macrophages (17)(18)(19). In addition, cholesterol-poor liposomes are known to interact with plasma lipoproteins and serve as a sink for cholesterol in particular from LDL as the main carrier of plasma cholesterol (20).…”

Section: Introductionmentioning

confidence: 99%

“…A c c e p t e d M a n u s c r i p t 18 M a n u s c r i p t Highlights  Apolipoprotein B-100 (apoB) is the major protein in the structure of atherogenic lipoproteins.  There is evidence on the strong interaction between cholesterol-free nanoliposomes and lipoproteins.…”

mentioning

confidence: 99%

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Apolipoprotein B-100-targeted negatively charged nanoliposomes for the treatment of dyslipidemia

Sahebkar

Badiee

Hatamipour

et al. 2015

Colloids and Surfaces B: Biointerfaces

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Section: Cytotoxicity Assaysmentioning

confidence: 99%

Section: Cytotoxicity Assaysmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Apolipoprotein B-100-targeted negatively charged nanoliposomes for the treatment of dyslipidemia

Sahebkar

Badiee

Hatamipour

et al. 2015

Colloids and Surfaces B: Biointerfaces

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“…In addition, anionic liposomes have been extensively studied as vehicles for the delivery of pharmaceutical cargo. 3) They may be used as a drug reservoir for controlled release, to reduce toxicities associated with drugs exhibiting narrow therapeutic indices, and to deliver drugs directly to target cells. 4) Cholest-5-en-3-ol-(3b) Fig.…”

Section: Introductionmentioning

confidence: 99%

Characterization of Transferrin-Modified Procationic-Liposome Protamine-DNA Complexes

et al. 2007

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We developed a novel transferrin modiˆed non-viral gene delivery system, transferrin-modiˆed procationicliposome-protamine-DNA complexes (Tf-PLPD) and investigated its characteristics. Blank procationic liposomes were prepared using theˆlm dispersionˆlter method. Protamine was used to condense plasmid DNA to form protamine-D-NA complexes and the complexes were further incubated with blank procationic liposomes to form PLPD. Transferrin was adsorbed onto the surface of PLPD via an electrostatic interaction, and thus Tf-PLPD was produced. Characteristics such as stability in rat serum, morphology, average particle size, zeta potential, and transfection e‹ciency in HepG2 cells were further investigated. The results indicated that the procationic liposomes remained stable in rat serum for 24 h. Tf-PLPD protected plasmid DNA from enzymatic degradation even after lyophilization. The size distribution of Tf-PLPD was in the range of 240±12 nm and the zeta potential was -24.10±2.5 mV (n＝3), respectively. The transfection e‹ciencies of Tf-PLPD were 24.26±2.6 mU b-galactosidase/mg protein. Lyophilization and the presence of serum did not aŠect the transfectivity of Tf-PLPD and the procationic liposomes also had low cytotoxicity to cells.

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Submicron‐Sized In Situ Osmotic Pressure Sensors for In Vitro Applications in Biology

Zhang

Bertinetti

Yavuzsoy

et al. 2023

Adv Healthcare Materials

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Dedicated to the memory of Helmuth Möhwald, who has been a much-respected colleague and mentor, as well as a pioneer in the research on functional microcapsules Physical forces are important cues in determining the development and the normal function of biological tissues. While forces generated by molecular motors have been widely studied, forces resulting from osmotic gradients have been less considered in this context. A possible reason is the lack of direct in situ measurement methods that can be applied to cell and organ culture systems. Herein, novel kinds of resonance energy transfer (FRET)-based liposomal sensors are developed, so that their sensing range and sensitivity can be adjusted to satisfy physiological osmotic conditions. Several types of sensors are prepared, either based on polyethylene glycol-(PEG)ylated liposomes with steric stabilization and stealth property or on crosslinked liposomes capable of enduring relatively harsh environments for liposomes (e.g., in the presence of biosurfactants). The sensors are demonstrated to be effective in the measurement of osmotic pressures in pre-osteoblastic in vitro cell culture systems by means of FRET microscopy. This development paves the way toward the in situ sensing of osmotic pressures in biological culture systems.

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LDL-induced association of anionic liposomes with cells and delivery of contents

Cited by 10 publications

References 23 publications

Apolipoprotein B-100-targeted negatively charged nanoliposomes for the treatment of dyslipidemia

Apolipoprotein B-100-targeted negatively charged nanoliposomes for the treatment of dyslipidemia

Characterization of Transferrin-Modified Procationic-Liposome Protamine-DNA Complexes

Submicron‐Sized In Situ Osmotic Pressure Sensors for In Vitro Applications in Biology

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