LDL fraction in rats‐fed trans fatty acids induces inflammatory factors in human umbilical vein endothelial cells (821.1)

Abstract

Trans‐fatty acid is a well‐known risk factor of atherogenesis, however its mechanism has not been clarified. Plasmalogen is a subclass of phospholipids, characterized by vinyl‐ether bond in sn‐1 position, and known to have the anti‐atherogenenic property. We have found that trans‐fatty acids were incorporated into sn‐1 position of plasmalogen (trans‐type plasmalogen) in trans‐fatty acids fed rats. The aim of this study was to examine the physiological effects of trans‐type plasmalogen on vascular endothelium. Wistar‐ST male rats were fed an AIN‐93G based diet with or without 23% hydrogenated rape seed oil (including 40% trans‐fatty acids) for 8 weeks, and then we prepared LDL fraction from their aorta blood. Human Umbilical Vein Endothelial Cells (HUVEC, p=4~6) were cultivated until the cells were 80% confluent and the medium was changed containing rat LDL, then HUVEC were cultivated for 24h. The gene expressions of ICAM1, VCAM1 and MCP1 in HUVEC were increased in trans LDL group (LDL including trans‐type plasmalogen) more than control LDL group. MCP1 protein in cell supernatant was also higher in the trans group. These results suggest that LDL including trans‐type plasmalogen has inflammatory effects in HUVEC. We should clarify that trans‐type plasmalogens actually contribute the inflammatory effects in LDL on HUVEC.