LDH isoenzyme pattern, lactate/pyruvate, and ultrastructure of fibrinogenic and epithelial cell lines exposed to 3% oxygen

Dehydrogenases in Local Carcinogenesis in Rat Mammary Gland by Dusting of Dmba-Powder

“…Recently, Lee et al (8) reported that the activity of LDH and the pattern of its isoenzymes could be utilized as biological markers for normal and neoplastic mammary tissues of Sprague-Dawley females at various stages of development, and that actively growing mammary tumors were proved to exhibit an extremely high activity of LDH and an elevated portion of the muscle type of subunits. Although it is unclear whether a change in LDH isoenzymes presents a cause or an effect in the development of the malignant cells, the shift toward predominance of the M type of LDH in the tumor tissue is consistent with and supportive of its altered metabolism to sustain the high rate of glycolytic activity (4,16). In present experiment, the predominance was distinctly detected in the earlier harvested granulomatous samples.…”

“…Histochemically, atypisms of ALP and LDH activities were observed on the apparent malignant tissues induced by DMBA-dusting. Biochemical observations on the activities of LDH and MDH and on the pattern of LDH isoenzymes were done for the induced nodules with DMBA-dusting over females aged [8][9][10][11][12][13][14][15][16][17][18][19][20][21][22][23][24][25][26] weeks, which were harvested 5-18 weeks after the local application. The quotients of LDH/MDH in the same sample elevated on the malignant tissues prominently owing to higher LDH activity, and the elevation was moderate on the earlier-harvested, granulomatous tissues.…”