LC-MS TECHNIQUE & ITS METABOLOMIC BASED APPROACHES IN SCIENCE AND TECHNOLOGY

Abstract

The metabolome is the complete set of metabolites found during a biological cell, tissue, organ or organism, representing the end product of cellular processes. Metabolomics is rising platform that enables one to research global endogenous metabolites with low molecular weight in biological systems. On the other hand, an acceptance of various analytical technique is predicated on procedures with successfully carried out for completely different types of metabolites on which Liquid chromatography (LC) in combination with tandem mass spectrometry (MS/MS) has enjoyed a growing quality. LC-MS is the best platform for metabolomic studies because of its glorious sensitivity, resolution, high throughput, soft ionization, and good coverage of metabolites. Also, an ability that can accurately and precisely discriminate target analytes from high complexity mixtures in a sensitive and selective way. In this review article we have discussed an omics and its different types, mainly focused on metabolomics and various application of metabolomics using LC-MS analytical technique. Here we collected all information regarding applications of metabolomics known by LC-MS. Highlight necessary pit falls in the process of metabolomics using LC-MS techniques and address multiple advantages of implementing LC-MS technique over another analytical techniques for metabolomics during a varied approaches.