LC-MS Quantification of Thyroid Hormones, Their Metabolites, Amino Acids and Neurotransmitters in Liver Tissue

Zanov, Kl.; Yotova, Lyubov; Klapa, Maria I.

doi:10.1080/13102818.2009.10818548

Biotechnology & Biotechnological Equipment

2009

DOI: 10.1080/13102818.2009.10818548

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LC-MS Quantification of Thyroid Hormones, Their Metabolites, Amino Acids and Neurotransmitters in Liver Tissue

Kl. Zanov

Lyubov Yotova

Maria I. Klapa

Abstract: ABSTRACT

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Cited by 2 publications

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References 28 publications

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“…Comparison between samples spiked before and after the homogenization step demonstrated that accuracy is mainly aff ected by the homogenization process. Further improvement of the method would be desirable, and this would require a deeper analysis of the mechanisms involved in the equilibrium regulating the partition of both T 3 and T 4 between homogenization medium and tissue [ 20 ] . Fig.…”

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confidence: 99%

Quantification of Thyroxine and 3,5,3′-Triiodo-Thyronine in Human and Animal Hearts by a Novel Liquid Chromatography-Tandem Mass Spectrometry Method

et al. 2014

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Assaying tissue T3 and T4 would provide important information in experimental and clinical investigations. A novel method to determine tissue T3 and T4 by HPLC coupled to mass spectrometry is described. The major difference vs. previously described methods lies in the addition of a derivatization step, that is, to convert T3 and T4 into the corresponding butyl esters. The yield of esterification was ̴ 100% for T3 and 80% for T4. The assay was linear (r>0.99) in the range of 0.2-50 ng/ml, accuracy was in the order of 70-75%, and the minimum tissue amount needed was in the order of 50 mg, that is, about one order of magnitude lower than observed with the same equipment (AB Sciex API 4000 triple quadrupole mass spectrometer) if derivatization was omitted. The method allowed detection of T3 and T4 in human left ventricle biopsies yielding concentrations of 1.51±0.16 and 5.94±0.63 pmol/g, respectively. In rats treated with different dosages of exogenous T3 or T4, good correlations (r>0.90) between plasma and myocardial T3 and T4 concentrations were observed, although in specific subsets different plasma T4 concentrations were not associated with different tissue content in T4. We conclude that this method could provide a novel insight into the relationship between plasma and tissue thyroid hormone levels.

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Quantification of Thyroxine and 3,5,3′-Triiodo-Thyronine in Human and Animal Hearts by a Novel Liquid Chromatography-Tandem Mass Spectrometry Method

et al. 2014

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Determination of thyroid hormones in mouse tissues by isotope-dilution microflow liquid chromatography–mass spectrometry method

Angelis

Giesert

Finan

et al. 2016

Journal of Chromatography B

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LC-MS Quantification of Thyroid Hormones, Their Metabolites, Amino Acids and Neurotransmitters in Liver Tissue

Abstract: ABSTRACT

Cited by 2 publications

References 28 publications

Quantification of Thyroxine and 3,5,3′-Triiodo-Thyronine in Human and Animal Hearts by a Novel Liquid Chromatography-Tandem Mass Spectrometry Method

Quantification of Thyroxine and 3,5,3′-Triiodo-Thyronine in Human and Animal Hearts by a Novel Liquid Chromatography-Tandem Mass Spectrometry Method

Determination of thyroid hormones in mouse tissues by isotope-dilution microflow liquid chromatography–mass spectrometry method

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