LC-MS/MS profiles and interrelationships between the enzyme inhibition activity, total phenolic content and antioxidant potential of Micromeria nervosa extracts

Sarıkürkçü, Cengiz; Andrade, Jacqueline Cosmo; Özer, Mehmet Sabih; Silva, João Marcos Ferreira de Lima; Ceylan, Olcay; Sousa, Erlânio Oliveira de; Coutinho, Henrique Douglas Melo

doi:10.1016/j.foodchem.2020.126930

Cited by 22 publications

(11 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In this study, the best antioxidant properties were obtained in the normal green shoot, followed by somaclonal variant shoot and callus extracts. These results can be attributed to the levels of phenolics in the extracts, as suggested by several researchers [67,68], who reported a positive correlation between the concentration of phenolics and their antioxidant properties. The metal chelating ability was ranked as somaclonal variant shoots > normal green shoots > callus.…”

Section: Discussionmentioning

confidence: 53%

Phytochemical Composition, Antioxidant Capacity, and Enzyme Inhibitory Activity in Callus, Somaclonal Variant, and Normal Green Shoot Tissues of Catharanthus roseus (L) G. Don

Lee

Zengin

et al. 2020

Molecules

View full text Add to dashboard Cite

This study aimed to investigate the impact of plant growth regulators, sucrose concentration, and the number of subcultures on axillary shoot multiplication, in vitro flowering, and somaclonal variation and to assess the phytochemical composition, antioxidant capacity, and enzyme inhibitory potential of in vitro-established callus, somaclonal variant, and normal green shoots of Catharanthus roseus. The highest shoot induction rate (95.8%) and highest number of shoots (23.6), with a mean length of 4.5 cm, were attained when the C. roseus nodal explants (0.6–1 cm in length) were cultivated in Murashige and Skoog (MS) medium with 2 µM thidiazuron, 1 µM 2-(1-naphthyl) acetic acid (NAA), and 4% sucrose. The in vitro flowering of C. roseus was affected by sucrose, and the number of subcultures had a significant effect on shoot multiplication and somaclonal variation. The highest levels of phenolics and flavonoids were found in normal green shoots, followed by those in somaclonal variant shoots and callus. The phytochemicals in C. roseus extracts were qualified using liquid chromatography–tandem mass spectrometry. A total of 39, 55, and 59 compounds were identified in the callus, somaclonal variant shoot, and normal green shoot tissues, respectively. The normal green shoot extracts exhibited the best free radical scavenging ability and reducing power activity. The strongest acetylcholinesterase inhibitory effects were found in the callus, with an IC50 of 0.65 mg/mL.

show abstract

Section: Discussionmentioning

confidence: 53%

Phytochemical Composition, Antioxidant Capacity, and Enzyme Inhibitory Activity in Callus, Somaclonal Variant, and Normal Green Shoot Tissues of Catharanthus roseus (L) G. Don

Lee

Zengin

et al. 2020

Molecules

View full text Add to dashboard Cite

show abstract

“…In another study, alpha amylase and tyrosinase enzyme and antioxidant activity of different extracts of M. nervosa were studied and the phenolic compounds in its content were analysed by LC-MS/MS [29]. As can be understood from the studies in the literature, there are very limited studies on the phenolic compound analysis on Micromeria species.…”

Section: Discussionmentioning

confidence: 99%

LC-ESI-tandem MS and in silico ADMET analysis of polyphenols from Rhus coriaria L. and Micromeria fruticosa (L.) Druce ssp. brachycalyx P. H. Davis

2021

View full text Add to dashboard Cite

Background Micromeria fruticosa (L.) Druce ssp. brachycalyx P. H. Davis and Rhus coriaria L., which are Lamiaceae species, are used both as spices in food and medicinally. Lamiaceae species are known to contain high amounts of polyphenols. In this study, liquid chromatography–quadrupole time-of-flight–tandem mass spectrometry (LC-QTOF-MS/MS) was used for analysis of polyphenols in the plants. Under gradient elution with using 0.1% aqueous acetic acid solution and acetonitrile mobile phases, an Agilent Poroshell C18 reversed phase column was used for the simultaneous determination of 18 polyphenols, and separation was performed in 30 min. Pharmacokinetic properties of these polyphenols such as drug-like and toxicity were estimated using open-source software, pkCSM and SwissADME. Results These compounds were determined to represent different classes of polyphenols, including phenolic acids, flavonoids, coumarin and tannins. ADMET predictions of polyphenols indicated that these compounds are easily absorbed and do not have toxic effects. Conclusion While the Rhus coriaria L. includes anthocyanidins, tannins, phenolic acid and flavonoids, the Micromeria fruticosa (L.) Druce ssp. brachycalyx P. H. Davis has phenolic acid, coumarin and flavonoids, according to these results. In silico ADME/Tox predictions revealed that these bioactive components are to be drug-like and non-mutagenic. These data are supportive for future analysis that can lead to their therapeutic use of the plants, suggesting that this species may be used as a natural medicinal source in the future after detailed analysis tests. Graphical abstract

show abstract

“…Phenolic compounds extracted from plants have been intensively investigated due to their benefi ts for human health (Tungmunnithum et al, 2018). Several studies have showed that these compounds possess antioxidant activity (Aryal et al, 2019;Dong et al, 2019;Kakouri et al, 2019;Sarikurkcu et al, 2020). Therefore, it is important to fi nd suitable methods of extraction of these compounds.…”

Section: Discussionmentioning

confidence: 99%

“…Ismail et al (2019) analyzed baobab (Adansonia digitata) fruit pulp, and concluded that, in this case, 80 % acetone is the best solvent. Sarikurkcu et al (2020) worked with ethyl acetate, methanolic, and aqueous extracts from Micromeria nervosa aerial parts, and concluded that the diff erent extracts possess diff erent mechanisms responsible for the antioxidant activity. Array et al (2018) worked with Curcuma longa and showed that the alcoholic extracts, obtained at room temperature, exhibited the highest phenolic content and antioxidant activities.…”

Section: Discussionmentioning

confidence: 99%

POLYPHENOL CONTENT AND ANTIOXIDANT POTENTIAL OF Allophylus edulis (A. St.-Hil. et al.) Hieron. ex Niederl. AND Cupania vernalis Cambess. (SAPINDACEAE)

et al. 2021

View full text Add to dashboard Cite

Allophylus edulis and Cupania vernalis (Sapindaceae) are Brazilian native trees used as medicinal plants for the treatment of respiratory, digestive, circulatory, and skin diseases. Ubiquitously distributed in the Brazilian territory, these species are indicated for mixed plantations aimed at the recovery of degraded ecosystems. In this study, the total phenolic content (TPC) and total flavonoid content (TFC), and the antioxidant activity of extracts and fractions obtained from A. edulis and C. vernalis leaves were assessed. The TPC and TFC was determined spectrophotometrically. Antioxidant activity was evaluated through radical scavenging activity of 2,2-diphenyl-1-picrylhydrazyl (DPPH). The extracts were obtained by two methods: maceration (method 1) and Soxhlet (method 2). Solvents of increasing polarity (hexane, dichloromethane, ethyl acetate, and n-butanol) were used to obtained the fractions. The results showed that the ethyl acetate fraction from A. edulis, obtained from the maceration method, had the highest TPC (442.0 ± 18.2 mg GAE g-1) and TFC (58.1 ± 0.4 mg RUE g-1), and antioxidant activity (EC50 = 43.6 ± 2.6 µg mL-1). By C. vernalis, superior results were obtained with the n-butanol fraction (TPC = 126.1 ± 5.8 mg GAE g-1, TFC = 37.7 ± 0.6 mg RUE g-1). The highest antioxidant potential was found in the crude hydroalcoholic extract (EC50 = 816.1 ± 50.9 µg mL-1) and butanol fraction (1,156.4 ± 3.8 µg mL-1). The results of this study show that the fractions obtained by maceration and liquid-liquid partition with more polar solvents (ethyl acetate and n-butanol) are the richest in TPC and TFC, and presented the greater antioxidant activity. Comparing the two plants, A. edulis showed the best results, with a high content of TPC, TFC, and antioxidant potential, and therefore may be used to treat diseases related to oxidative stress.

show abstract

LC-MS/MS profiles and interrelationships between the enzyme inhibition activity, total phenolic content and antioxidant potential of Micromeria nervosa extracts

Cited by 22 publications

References 29 publications

Phytochemical Composition, Antioxidant Capacity, and Enzyme Inhibitory Activity in Callus, Somaclonal Variant, and Normal Green Shoot Tissues of Catharanthus roseus (L) G. Don

Phytochemical Composition, Antioxidant Capacity, and Enzyme Inhibitory Activity in Callus, Somaclonal Variant, and Normal Green Shoot Tissues of Catharanthus roseus (L) G. Don

LC-ESI-tandem MS and in silico ADMET analysis of polyphenols from Rhus coriaria L. and Micromeria fruticosa (L.) Druce ssp. brachycalyx P. H. Davis

POLYPHENOL CONTENT AND ANTIOXIDANT POTENTIAL OF Allophylus edulis (A. St.-Hil. et al.) Hieron. ex Niederl. AND Cupania vernalis Cambess. (SAPINDACEAE)

Contact Info

Product

Resources

About