LC-MS/MS metabolomics-facilitated identification of the active compounds responsible for anti-allergic activity of the ethanol extract of Xenostegia tridentata

Abstract

In vivo and in vitro anti-allergic activities of ethanol extract of Xenostegia tridentata (L.) D.F. Austin & Staples were investigated using passive cutaneous anaphylaxis reaction assay and RBL-2H3 cell degranulation assay, respectively. The crude ethanol extract exhibited promising activities when compared with the known anti-allergic agents, namely dexamethasone and ketotifen fumarate. The ethyl acetate subfraction showed the highest anti-allergic activity among various sub-partitions and showed better activity than the crude extract, consistent with the high abundance of total phenolic and flavonoid contents in this subfraction. LC-MS/MS metabolomics analysis and bioassay-guided isolation were then used to identify chemical constituents responsible for the anti-allergic activity. The results showed that major components of the ethyl acetate subfraction consist of 3,5-dicaffeoylquinic acid, quercetin-3-O-rhamnoside, kaempferol-3-O-rhamnoside and luteolin-7-O-glucoside. The inhibitory activity of the isolated compounds against mast cell degranulation was validated, ensuring their important roles in the anti-allergic activity of the plant. Notably, besides showing the anti-allergic activity of X. tridentata, this work highlights the role of metabolomic analysis in identifying and selectively isolating active metabolites from plants.

Anti-Allergic and Antioxidant Potential of Polyphenol-Enriched Fractions from Cyclopia subternata (Honeybush) Produced by a Scalable Process

“…Glycosylation had a severe negative effect on the activity of flavones and flavonols in the study by Mastuda et al [12]. However, Suntivich et al [38] showed ca. 50% inhibition of mast cell degranulation for the glycosylated flavone luteoloside at 25 µM.…”

Photocatalytic and Biological Activities of Spherical Shape Cellulose/Silver Nanocomposites Using Xenostegia tridentata (L.) Leaf Extract

“…An FT-IR spectrum had been shown with the aid of using the presence of silver nanoparticles in the cellulose, XTL (leaf ), as shown in Figures 6(a Tese bands can be attributed to 3,5-dicafeoylquinic acid, quercetin-3-o-rhamnoside, kaempferol-3-o-rhamnoside, luteolin-7-o-glucoside, β-sterol, and stigmasterol compounds which might be considerably found in Xenostegia tridentata (L.) leaf extract [12][13][14] and are responsible for the reduction of silver ion to silver nanoparticles in the cellulose matrix.…”

“…Te Xenostegia tridentata (L.) possesses good diuretic, antiallergic, bitter, astringent, calefacient, laxative, purgative, fever, snake bite, tonic, and spasmolytic characteristics [10,11]. Xenostegia tridentata (L.) leaf has been found to contain 3,5-cafeoylquinic acid, quercetin-3-o-rhamnoside, kaempferol-3-o-rhamnoside, luteolin-7-o-glucoside, β-sterol, and stigma sterol compounds that can be capping and reducing agents (XTLLs) which can easily reduce the silver nitrate solution to Ag ions in cellulose matrix [12][13][14]. In previous studies, the radical scavenging activities of silver nanoparticles and cellulose silver nanocomposites have been reported as a free radical scavenger in in vivo and in vitro systems [15][16][17].…”

Enhancing Glycosylation of Flavonoids by Engineering the Uridine Diphosphate Glucose Supply in Escherichia coli

“…Flavonoid glycosides can be divided into flavonoid O - and C -glycosides according to the flavonoid and the glycosyl group connected via C–O and C–C bonds . Vitexin (apigenin-8- C -glucoside), orientin (luteolin-8- C -glucoside), afzelin (kaempferol-3- O -rhamnoside), quercitrin (quercetin-3- O -rhamnoside), trifolin (kaempferol-3- O -galactoside), and hyperoside (quercetin-3- O -galactoside) are natural flavonoid glycosides in plants and active ingredients in many Chinese herbs. − These flavonoid glycosides are receiving increasing attention as a result of their broad pharmacological effects, including antioxidant, neuroprotective, anticancer, antihyperalgesic, and anti-inflammatory activities. − …”