LC-MS/MS determination and pharmacokinetics study of puerarin and daidzein in rat plasma after oral administration of Gegenqinlian decoction and Radix Puerariae extract

Abstract

Background:Gegenqinlian decoction (GQD) is a famous traditional medicine recipe. It is composed of four herbs including Radix Puerariae (GG), Radix Scutellariae (HQ), Rhizoma Coptidis (HL) and Radix Glycyrrhizae (GC), which is widely used for treating gastro-intestinal disorders in the clinical practice of Traditional Chinese medicine (TCM). The aim of this study was to compare the pharmacokinetics of puerarin and daidzein in rats following oral administration of Gegenqinlian Decoction and Radix Puerariae extract. Thus, a sensitive and selective liquid chromatography/tandem mass spectrometry (LC-MS/MS) method was developed and validated for simultaneous determination of puerarin and daidzein in rat plasma following oral administration of Gegenqinlian Decoction and Radix Puerariae extract.Materials and Methods:Chromatographic separation was performed on a Shiseido CAPCELL PAK C18 analytical column (100 mm × 2.0 mm i.d., 5 μm) by linear gradient elution, with water (0.1% formic acid)-acetonitrile (0.1% formic acid) as mobile phase. Detection was carried out by multiple reaction monitoring (MRM) mode using electrospray ionization in the positive ion mode.Results:The calibration curves were linear over a range of 7.80-1560 ng/mL for puerarin and 6.30-1260 ng/mL for daidzein. The intra- and inter-day precision values were less than 13.6% and their average recoveries was in the range of 77.8% and 88.6% for puerarin and was between 76.3 and 86.8% for daidzein, respectively.Conclusion:The validated method was applied to the comparative pharmacokinetic studies of puerarin and daidzein after oral administration of Gegenqinlian Decoction and Radix Puerariae extract. The pharmacokinetic parameters showed that puerarin and daidzein from Gegenqinlian Decoction were absorbed more effectively with slower elimination in rat plasma than that from Radix Puerariae extract. These results revealed that as far as the Radix Puerariae extract was concerned, it is very valuable to be used as a clinical directions of Gegenqinlian Decoction.

Pharmacokinetics and Bioavailability of the Isoflavones Formononetin and Ononin and Their in Vitro Absorption in Ussing Chamber and Caco-2 Cell Models

“…The first peak was observed at around 30 min, indicating a rapid absorption of ononin, and then experienced a quick decline and a subsequently small rise that peaked at 4 h after oral administration (Figure ). The rapid absorption of formononetin and ononin was observed similar to other reported isoflavones such as genistein, daidzein, daidzin, and puerarin with T max of 0.25–1 h. − Furthermore, consistent with previous studies, , the double-peak phenomenon of ononin has been reported after oral administration of other herbal extracts, which is further discussed in the next section after the gut permeability study.…”

The High Content of Quercetin and Catechin in Airen Grape Juice Supports Its Application in Functional Food Production

“…The separation of the compounds by LC was accomplished following the elution conditions described in the Materials and Methods section. For the quantification by MS, a database of 56 grape polyphenols with the MS parameters necessary for their identification was created using the data previously published [ 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 , 65 , 66 , 67 ] ( Supplementary Materials, Table S3 ). Twenty-three of these polyphenols were selected for the study, and 15 have been identified in the extracts ( Supplementary Materials, Table S2 ).…”

Pharmacokinetics and drug delivery systems for puerarin, a bioactive flavone from traditional Chinese medicine

“…The compatible herbs in the prescriptions could influence the pharmacokinetics of puerarin. The pharmacokinetic parameters of puerarin from Gegenqinlian decoction were absorbed more effectively with slow elimination in rat plasma than from Radix Puerariae extracts (Zhang et al., 2014).…”