LC/MS/MS determination and pharmacokinetic studies of six compounds in rat plasma following oral administration of the single and combined extracts of Eucommia ulmoides and Dipsacus asperoides

Huang, Yuxing; Liu, Erwei; Wang, Lei; Huo, Yan; Wang, Qiang; Olaleye, Olajide E.; Wang, Tao; Gao, Xiumei

doi:10.1016/s1875-5364(14)60073-x

Cited by 13 publications

(11 citation statements)

References 16 publications

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“…It is necessary to provide an integrated absorbed and metabolic profile for a scientific understanding of the therapeutic effects as well as theories about TCM formulations. At present, researchers are still focusing on the individual chemical composition and herbs of TGG in vivo (Du et al, ; Huang et al, ; Tang et al, ; Zhao, Qi, Chen, Sun, & Yuan, ). However, to the best of our knowledge, based on overall and systematic experiments, there have been few reports on the absorbed prototype components and the metabolites of TGG.…”

Section: Introductionmentioning

confidence: 99%

Identification of the absorbed components and their metabolites of Tianma‐Gouteng granule in rat plasma and bile using ultra‐high‐performance liquid chromatography combined with quadrupole time‐of‐flight mass spectrometry

Zhang

Duan

Wang

et al. 2019

Biomedical Chromatography

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Tianma‐Gouteng granule (TGG), a Chinese herbal formula preparation, is clinically used for the treatment of cardio‐cerebrovascular diseases such as hypertension, cerebral ischaemia, acute ischaemic stroke and Parkinson's disease. Although few reports have been published concerning the absorbed prototype components of TGG, the possible metabolic pathways of TGG in vivo remain largely unclear. In this study, a method using UPLC–Q/TOF MS was established for the detection and identification of the absorbed prototype components and related metabolites in rat plasma and bile after oral administration of TGG at high and normal clinical dosages. A total of 68 components were identified or tentatively identified in plasma and bile samples, including absorbed prototypes and their metabolites. The major absorbed components were gastrodin, isorhynchophylline, rhynchophylline, isocorynoxeine, corynoxeine, geissoschizine methyl ether baicalin, baicalein, wogonoside, wogonin, geniposidic acid, leonurine, 2,3,5,4′‐tetrahydroxystilbene‐2‐O‐β‐d‐glucoside and emodin. The main metabolic pathways of these components involved phase I (isomerization, hydrolysis and reduction) and phase II (glucuronidation and sulfation) reaction, and the phase II biotransformation pathway was predominant. The present study provides rich information on the in vivo absorption and metabolism of TGG, and the results will be helpful for further studies on the pharmacokinetics and pharmacodynamics of TGG.

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Section: Introductionmentioning

confidence: 99%

Identification of the absorbed components and their metabolites of Tianma‐Gouteng granule in rat plasma and bile using ultra‐high‐performance liquid chromatography combined with quadrupole time‐of‐flight mass spectrometry

Zhang

Duan

Wang

et al. 2019

Biomedical Chromatography

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“…Various chromatographic methods have been published for the determination of GE or GP in combination with other compounds in the biological matrix using HPLC‐UV or LC–MS/MS (Chen et al, ; Cheng, Lin, Lin, & Tsai, ; He et al, ; Hu et al, ; Huang et al, ; Peng et al, ; Qu et al, , ; Wang et al, ). Some methods have been established for simultaneous quantification of GE and GP.…”

Section: Introductionmentioning

confidence: 99%

A sensitive LC–MS/MS method for simultaneous quantification of geniposide and its active metabolite genipin in rat plasma and its application to a pharmacokinetic study

Shi

Pan

et al. 2017

Biomedical Chromatography

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Genipin (GP), an active metabolite of geniposide (GE), exhibits more potent pharmacological effects than its parent compound. In this paper, a sensitive LC-MS/MS method was developed and fully validated for the simultaneous determination of GE and GP in rat plasma. We found that GP degraded rapidly in rat plasma at room temperature as a result of irreversible binding with the endogenous nucleophiles in plasma. GP was stable when the sample's pH was ≤4.0. The degradation of GP in rat plasma was well prevented by immediate addition of 5% glacial acetic acid to the freshly collected plasma. The detection was performed on a tandem mass spectrometer coupled with electrospray ionization source in negative mode. Quantification was conducted by multiple reaction monitoring of the transitions [M + CH COO] m/z 447.3 → 225.3 for GE and [M - H] m/z 225.2 → 123.1 for GP. The method exhibited high sensitivity (LLOQ 1 ng/mL for GE and 0.2 ng/mL for GP) by selecting the acetate adduct ions as the precursor ions for GE. The robust developed method was successfully applied to a pharmacokinetic study in rats after oral administration of GE.

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“…Dipsacus species are native to Europe, Asia, and northern Africa (Zhang et al 1997). Dipsacus asperoides is distributed widely in the southwestern of China and has been used as the medicinal herb to treat lumbar and knee pain, liver dysfunction, traumatic hematoma, threatened abortion, and bone fractures (Cong et al 2013;Huang et al 2014). However, there has been no study on the genomic data of D. asperoides that limited to research genetic engineering and natural populations of this species.…”

Section: Dipsacus Asperoides;mentioning

confidence: 99%

Phylogenetic analysis and characterization of the complete chloroplast genome of Dipsacus asperoides, the endemic medicinal herb in China

Cao

Fei

et al. 2019

Mitochondrial DNA Part B

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Dipsacus asperoides is a traditional and endemic medicinal herb in China. Its roots was considered top grade herb as early as in the Shen Nong Herbal Classic. In this study, we assembled and analyzed the complete chloroplast genome of D. asperoides. The complete chloroplast genome is 160,481 bp in length, exhibiting a large single-copy region (88,546 bp), a small single-copy region (19,671 bp), and two inverted-repeat regions (26,132 bp in each one). The chloroplast genome of D. asperoides contains 133 genes, including 89 protein-coding genes (PCGs), 36 transfer RNA (tRNAs), and eight ribosome RNA (rRNAs). The overall nucleotide content of the chloroplast genome is A of 30.2%, T of 31.0%, C of 19.7%, and G of 19.1%, with a total AT content of 61.2% and GC content of 38.8%. However, the phylogenetic Maximum-Likelihood (ML) analysis based on the amino acid sequences of 89 PCGs from 14 species chloroplast genome that D. asperoides is closely related to Dipsacus asper. This study can be used for medicinal herb value research and clinical drug development.

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LC/MS/MS determination and pharmacokinetic studies of six compounds in rat plasma following oral administration of the single and combined extracts of Eucommia ulmoides and Dipsacus asperoides

Cited by 13 publications

References 16 publications

Identification of the absorbed components and their metabolites of Tianma‐Gouteng granule in rat plasma and bile using ultra‐high‐performance liquid chromatography combined with quadrupole time‐of‐flight mass spectrometry

Identification of the absorbed components and their metabolites of Tianma‐Gouteng granule in rat plasma and bile using ultra‐high‐performance liquid chromatography combined with quadrupole time‐of‐flight mass spectrometry

A sensitive LC–MS/MS method for simultaneous quantification of geniposide and its active metabolite genipin in rat plasma and its application to a pharmacokinetic study

Phylogenetic analysis and characterization of the complete chloroplast genome of Dipsacus asperoides, the endemic medicinal herb in China

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