LC/MS/MS detection of fungicide guazatine residues for quality assessment of commercial citrus fruit

Scordino, Monica; Sabatino, Leonardo; Traulo, Pasqualino; Gagliano, Giacomo; Gargano, Maria Letizia; Pantò, Valentina; Gambino, Grazia Laura

doi:10.1007/s00217-008-0849-3

Cited by 29 publications

(14 citation statements)

References 7 publications

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“…Now, there is a global trend on limiting chemical fungicides use in grains and food stuffs due to their toxicity, as their residues migrate into the food chain, leading to serious health hazards for consumers (Chen and others ; Scordino and others ) and the resistances in the fungi (Isaac, ). In recent years, many plant‐based natural products which are safer than synthetic products are considered as an alternative to these chemical fungicides and preservatives.…”

Section: Discussionmentioning

confidence: 99%

Inhibitory Effect of Cinnamaldehyde, Citral, and Eugenol on Aflatoxin Biosynthetic Gene Expression and Aflatoxin B₁ Biosynthesis in Aspergillus flavus

Liang

Xing

Selvaraj

et al. 2015

Journal of Food Science

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In order to reveal the inhibitory effects of cinnamaldehyde, citral, and eugenol on aflatoxin biosynthesis, the expression levels of 5 key aflatoxin biosynthetic genes were evaluated by real-time PCR. Aspergillus flavus growth and AFB1 production were completely inhibited by 0.80 mmol/L of cinnamaldehyde and 2.80 mmol/L of citral. However, at lower concentration, cinnamaldehyde (0.40 mmol/L), eugenol (0.80 mmol/L), and citral (0.56 mmol/L) significantly reduced AFB1 production with inhibition rate of 68.9%, 95.4%, and 41.8%, respectively, while no effect on fungal growth. Real-time PCR showed that the expressions of aflR, aflT, aflD, aflM, and aflP were down-regulated by cinnamaldehyde (0.40 mmol/L), eugenol (0.80 mmol/L), and citral (0.56 mmol/L). In the presence of cinnamaldehyde, AflM was highly down-regulated (average of 5963 folds), followed by aflP, aflR, aflD, and aflT with the average folds of 55, 18, 6.5, and 5.8, respectively. With 0.80 mmol/L of eugenol, aflP was highly down-regulated (average of 2061-folds), followed by aflM, aflR, aflD, and aflT with average of 138-, 15-, 5.2-, and 4.8-folds reduction, respectively. With 0.56 mmol/L of citral, aflT was completely inhibited, followed by aflM, aflP, aflR, and aflD with average of 257-, 29-, 3.5-, and 2.5-folds reduction, respectively. These results suggest that the reduction in AFB1 production by cinnamaldehyde, eugenol, and citral at low concentration may be due to the down-regulations of the transcription level of aflatoxin biosynthetic genes. Cinnamaldehyde and eugenol may be employed successfully as a good candidate in controlling of toxigenic fungi and subsequently contamination with aflatoxins in practice.

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Section: Discussionmentioning

confidence: 99%

Inhibitory Effect of Cinnamaldehyde, Citral, and Eugenol on Aflatoxin Biosynthetic Gene Expression and Aflatoxin B₁ Biosynthesis in Aspergillus flavus

Liang

Xing

Selvaraj

et al. 2015

Journal of Food Science

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“…This technique provides excellent sensitivity/selectivity and discriminates analyte and matrix signal more efficiently than GC-MS (Alder et al 2006) or LC-MS (Hernandez et al 2006). In recent years, a number of reliable LC-MS/MS methods have been developed for pesticide residue analysis for the postharvest fungicide residues (thiabendazole, carbendazim, thiophanate-methyl, imazalil and prochloraz) in citrus juices (Dreassi et al 2010), for guazatine in commercial citrus fruits (Scordino et al 2008), for thiosultap sodium, thiocyclam, and nereistoxin in pepper (Ferrer et al 2010), for multi-class pesticides residues in fresh grape samples (Venkateswarlu et al 2007) and in olives (Ferrer et al 2005).…”

Section: Introductionmentioning

confidence: 99%

Pesticide residue analysis in parsley, lettuce and spinach by LC-MS/MS

2011

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In this study, pesticide residues in parsley, lettuce and spinach (120 samples) were analyzed by the application of liquid chromatography-tandem mass spectrometry (LC-MS/MS). All samples of spinach, parsley or lettuce contained residues of three or more active substances. In parsley, carbendazim (100.0%), dichlorvos (100.0%), fenarimol (40.0%), pendimethalin (95.0%), in lettuce, diazinon (30.0%), dichlorvos (100.0%), pendimethalin (92.5%) phenthoate (12.5%), and in spinach, carbendazim (45.0%), cymoxanil (85.0%), dichlorvos (100.0%) and fenarimol (85.0%) were the significant active compounds. The maximum residue limits were exceeded in 28, 20 and 40 samples of parsley, lettuce and spinach, respectively. The results showed that there was a high occurrence of pesticide residues in parsley, lettuce and spinach samples from Hatay province, in which most of them were prohibited from use in Turkey for these vegetables. The contamination levels of these residues may be considered a serious public health problem according to the maximum residue limits (MRLs) of Turkey and the European Union (EU).

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“…No time-consuming extraction procedure and toxic derivatization reagents were used, making the analysis appropriate for routine application. This screening should be carried out as routine analysis to assess quality of European waxes and could be integrated with the detection of unpermitted waxes and fungicides [18,19], preserving consumers and importers from commercial illicit and from potential toxicological consequences.…”

Section: Methods Applicationmentioning

confidence: 99%

LC/MS/MS detection of short-chain aliphatic amines in glazing agents for fruit coating

Sabatino

Scordino

Caruso

et al. 2012

Eur Food Res Technol

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An analytical protocol was developed for investigating short-chain aliphatic amines occurrence in waxes for fruit coating with the aim of controlling their commercialization in countries where the use of these carriers is forbidden. Six short-chain aliphatic amines (morpholine, diethanolamine, triethylamine, 2-dimethylaminoethanol, triethanolamine and 3-metoxypropylamine) were detected by LC/MS/MS operating in positive ion mode in fortified waxes after a two-step clean-up procedure. The analytical method was statistically validated. The matrix-matched regression lines showed r 2 [ 0.97. Recoveries ranging from 92 to 114 % were obtained for the fortification level of 3.5 % w/w and the relative standard deviations ranged from 2 to 8 % (n = 6). The limits of detection were below 0.2 % w/w, while the limits of quantification did not exceed 0.5 % w/w. The method is currently applied in ICQRF Laboratory of Catania on samples of coating waxes collected in the Italian market in the frame of MIPAAF institutional quality control activity.

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LC/MS/MS detection of fungicide guazatine residues for quality assessment of commercial citrus fruit

Cited by 29 publications

References 7 publications

Inhibitory Effect of Cinnamaldehyde, Citral, and Eugenol on Aflatoxin Biosynthetic Gene Expression and Aflatoxin B₁ Biosynthesis in Aspergillus flavus

Inhibitory Effect of Cinnamaldehyde, Citral, and Eugenol on Aflatoxin Biosynthetic Gene Expression and Aflatoxin B₁ Biosynthesis in Aspergillus flavus

Pesticide residue analysis in parsley, lettuce and spinach by LC-MS/MS

LC/MS/MS detection of short-chain aliphatic amines in glazing agents for fruit coating

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LC/MS/MS detection of fungicide guazatine residues for quality assessment of commercial citrus fruit

Cited by 29 publications

References 7 publications

Inhibitory Effect of Cinnamaldehyde, Citral, and Eugenol on Aflatoxin Biosynthetic Gene Expression and Aflatoxin B1 Biosynthesis in Aspergillus flavus

Inhibitory Effect of Cinnamaldehyde, Citral, and Eugenol on Aflatoxin Biosynthetic Gene Expression and Aflatoxin B1 Biosynthesis in Aspergillus flavus

Pesticide residue analysis in parsley, lettuce and spinach by LC-MS/MS

LC/MS/MS detection of short-chain aliphatic amines in glazing agents for fruit coating

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Product

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Inhibitory Effect of Cinnamaldehyde, Citral, and Eugenol on Aflatoxin Biosynthetic Gene Expression and Aflatoxin B₁ Biosynthesis in Aspergillus flavus

Inhibitory Effect of Cinnamaldehyde, Citral, and Eugenol on Aflatoxin Biosynthetic Gene Expression and Aflatoxin B₁ Biosynthesis in Aspergillus flavus